<i>Burkholderia cepacia</i>
            Complex Bacteria from Clinical and Environmental Sources in Italy: Genomovar Status and Distribution of Traits Related to Virulence and Transmissibility

Bevivino, Annamaria; Dalmastri, Claudia; Tabacchioni, Silvia; Chiarini, Luigi; Belli, Maria L.; Piana, S.; Materazzo, Alberto; Vandamme, Peter; Manno, G.

doi:10.1128/jcm.40.3.846-851.2002

Cited by 87 publications

(73 citation statements)

References 37 publications

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“…Two genetic markers have been associated with transmissibility and virulence of B. cepacia complex strains among CF patients: (i) the cblA gene, coding for the giant cable pili, which is believed to mediate the adherence of the bacterium to the respiratory epithelium (24,28) and (ii) the B. cepacia epidemic strain marker (BCESM), a conserved 1.4-kb open reading frame (18). However, they cannot be considered absolute markers of virulence and transmissibility since they may be absent in certain strains or genomovars and other factors appear to be important (4,6,17,20).…”

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confidence: 99%

Molecular Analysis of Burkholderia cepacia Complex Isolates from a Portuguese Cystic Fibrosis Center: a 7-Year Study

Cunha¹,

Leitão²,

Mahenthiralingam

et al. 2003

J Clin Microbiol

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110

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This work reports results of a systematic molecular analysis involving 113 Burkholderia cepacia complex isolates obtained from 23 cystic fibrosis (CF) patients under surveillance over a 7-year period at the major Portuguese CF center, the Santa Maria Hospital in Lisbon. The majority of the isolates were serial isolates from persistently infected patients (more than one-half of the population examined). In agreement with previous studies, B. cenocepacia (formerly genomovar III) was the most prevalent species; it was isolated from 52% of the patients infected with B. cepacia complex isolates. Contrasting with previous studies, a very significant percentage of the Portuguese CF subpopulation examined was infected with B. cepacia genomovar I (36%) and B. stabilis (18%). B. multivorans was recovered from two of the infected patients. All four of the species or genomovars were associated with poor clinical outcome, including the cepacia syndrome, and gave rise to chronic and transient infections, with the clinical condition depending on the patient and other still-unidentified factors. The B. cepacia epidemic strain marker region was found exclusively in genomovar III strains, while cblA was detected in genomovars I and III, only. There was no clear relation between the presence of these markers and transmissibility. Altogether, our results indicate that the use of these markers or the genomovar status in identifying patients at higher risk for infection is uncertain.

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confidence: 99%

Molecular Analysis of Burkholderia cepacia Complex Isolates from a Portuguese Cystic Fibrosis Center: a 7-Year Study

Cunha¹,

Leitão²,

Mahenthiralingam

et al. 2003

J Clin Microbiol

Self Cite

110

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“…Very little, however, is known about the potential pathogenicity of such strains recommended for application in an open environment, leading to the expression of profound concerns about the wisdom of such releases (15,22). Of particular concern are recent reports that the virulent genomovar III and other strains can be isolated from soil and cannot be easily differentiated from environmental isolates (2,23).…”

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confidence: 99%

Energy-Generating Enzymes ofBurkholderia cepaciaand Their Interactions with Macrophages

et al. 2003

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We previously demonstrated that several clinical and environmental isolates of Burkholderia cepacia secreted ATP-utilizing enzymes to the medium; the secretion of these enzymes by cystic fibrosis lung isolate strain 38 was shown to be greatly enhanced in the presence of ␣ 2 -macroglobulin. Fractionation of the growth medium of cystic fibrosis isolate strain 71 belonging to genomovar I demonstrated the presence of two additional proteins, homologues of Pseudomonas aeruginosa azurin and cytochrome c 551 , which are normally involved in electron transfer during denitrification. A Q-Sepharose column flowthrough fraction of the growth medium of B. cepacia strain 71 enriched with the azurin and cytochrome c 551 homologues triggered apoptosis in macrophages and mast cells, leading to their death. Incubation of the Q-Sepharose column flowthrough fraction with antiazurin and anti-cytochrome c 551 antibodies greatly reduced cell death. We cloned and hyperexpressed a gene from B. cepacia strain 71 that encodes the homologue of P. aeruginosa azurin. Such azurin homologues were detected in the growth medium of several strains belonging to genomovars I, III, and VI but not in the growth medium of strains belonging to other genomovars. The growth medium of the strains that elaborated the azurin homologue had high cytotoxicity towards macrophages. Purified azurin homologue was shown to induce apoptosis in macrophages in a caspase-dependent manner and was localized in both the cytosol and nucleus when incubated with or microinjected into macrophages. This is an interesting example of the interaction of a bacterial protein normally involved in cellular energetics with macrophages to effect their cell death.

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“…Thus giving extreme value to the proper identification of B. cepacia. (9)(10)(11)(12)(13)(14)(15)(16) The present study aimed to detect the isolation percentage of Bcc from the patients were admitted to burn units and ICUs of the Alexandria Main University Hospital throughout a period of 6 months (April to October 2015) and to determine their antibiotic susceptibility pattern.…”

Section: Introductionmentioning

confidence: 99%

“…and Pandoraea spp. (14,15) It is also associated with a wide variety of infections among hospitalized patients who are usually infected via contaminated equipment during their hospitalization such as indwelling catheters, ventilators, urethral instrumentation or exposure to contaminated solutions including antiseptics, disinfectants, nebulizer fluids and dextrose solutions. The infections usually include pneumonia, bacteremia, skin and soft tissue infection and genitourinary tract infection.…”

Section: Introductionmentioning

confidence: 99%

Existence of Burkholderia cepacia at Burn and Intensive Care Units in Alexandria Main University Hospital, Egypt

El-Barrawy¹

2017

jmscr

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Burkholderia cepacia Complex Bacteria from Clinical and Environmental Sources in Italy: Genomovar Status and Distribution of Traits Related to Virulence and Transmissibility

Cited by 87 publications

References 37 publications

Molecular Analysis of Burkholderia cepacia Complex Isolates from a Portuguese Cystic Fibrosis Center: a 7-Year Study

Molecular Analysis of Burkholderia cepacia Complex Isolates from a Portuguese Cystic Fibrosis Center: a 7-Year Study

Energy-Generating Enzymes ofBurkholderia cepaciaand Their Interactions with Macrophages

Existence of Burkholderia cepacia at Burn and Intensive Care Units in Alexandria Main University Hospital, Egypt

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