<i>Burkholderia fungorum</i>Septicemia

Gerrits, G. P. J. M.; Klaassen, Corné H. W.; Coenye, Tom; Vandamme, Peter; Meis, Jacques F.

doi:10.3201/eid1107.041290

Cited by 35 publications

(34 citation statements)

References 6 publications

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“…In contrast, B. fungorum, B. glathei, and B. sordidicola have been described as fungus-associated Burkholderia spp. (10,19,22,38). This shift toward fungusassociated species as roots progress from the juvenile to the senescent stage might be linked to the rapid decay of senescent roots, which constitute an ideal niche for organic matter-degrading fungi.…”

Section: Resultsmentioning

confidence: 99%

Burkholderia Species Are Major Inhabitants of White Lupin Cluster Roots

Weisskopf

Heller

Eberl

2011

Appl Environ Microbiol

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The formation of cluster roots by plants represents a highly efficient strategy for acquisition of sparingly available phosphate. This particular root type is characterized by a densely branched structure and high exudation of organic acids and protons, which are likely to influence the resident bacterial community. Until now, the identity of the bacterial populations living in cluster roots has not been investigated. We applied cultivation-dependent and cultivation-independent methods to characterize the dominant bacterial genera inhabiting the growing cluster roots of white lupin. We observed a high relative abundance of Burkholderia species (up to 58% of all isolated strains and 44% of all retrieved 16S rRNA sequences) and a significant enrichment with increasing cluster root age. Most of the sequences retrieved clustered together with known plant-or fungus-associated Burkholderia species, while only one of 98 sequences was affiliated with the Burkholderia cepacia complex. In vitro assays revealed that Burkholderia strains were much more tolerant to low pH than non-Burkholderia strains. Moreover, many strains produced large amounts of siderophores and were able to utilize citrate and oxalate as carbon sources. These features seem to represent important traits for the successful colonization and maintenance of Burkholderia species in white lupin cluster roots.To access sparingly available nutrients such as phosphate, plants have evolved several strategies, e.g., mycorrhizal association and cluster root formation. Cluster roots are very densely branched root structures with a particular excretion physiology (20,21,25). They occur in many species of the Proteaceae family and occasionally in the Mimosaceae, Casuarinaceae, or Fabaceae (7). White lupin is the only cluster-rooted species of agricultural importance and has thus been extensively studied (9,17,26,32,33,41,42). In white lupin, cluster root development follows a well-defined pattern: at the juvenile stage, cluster roots secrete small amounts of malate; at the mature stage, high quantities of citrate and protons are excreted, leading to drastic rhizosphere acidification; and at the senescent stage, organic acid excretion decreases. Besides citrate and malate, oxalate and fumarate have also been reported to be exuded by soil-grown lupin plants (6, 41). The close vicinity of growing cluster roots constitutes a highly selective environment, owing to rapid changes in pH and carbon availability. The abundance of bacteria, as well as richness and diversity, has been shown to decrease temporarily in mature cluster roots (40). However, the identity of the populations repressed or enriched during cluster root development has yet to be elucidated. We analyzed the bacterial communities living in the direct vicinity of cluster roots (root surface and inner tissues) by sequencing isolated strains and clone libraries constructed from root-extracted DNA. We then tested relevant physiological properties of isolates to better understand which metabolic abilities might ena...

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Section: Resultsmentioning

confidence: 99%

Burkholderia Species Are Major Inhabitants of White Lupin Cluster Roots

Weisskopf

Heller

Eberl

2011

Appl Environ Microbiol

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“…Six (10.5%) of 57 patients received quinolone-based regimens, of whom four received ciprofloxacin either alone [43] or combined with cefoperazone [76], vancomycin/tobramycin [68] and intravitreal ceftazidime [61] in each case. Of the remaining two patients, the first was treated with levofloxacin combined with cefoperazone/sulbactam [40] and the second with a non-specified quinolone [71].…”

Section: Case Reportsmentioning

confidence: 99%

Therapeutic options for Burkholderia cepacia infections beyond co-trimoxazole: a systematic review of the clinical evidence

Avgeri

Matthaiou

Dimοpoulos

et al. 2009

International Journal of Antimicrobial Agents

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“…In spite of this, retaining the statement 'The species are not associated with humans.' (Sawana et al, 2014) in the description of the genus Paraburkholderia would not be correct; several strains of Paraburkholderia fungorum were isolated from human clinical samples (Coenye et al, 2001;Gerrits et al, 2005). Such a statement should not be included into the description of the genus Caballeronia gen. nov. either; strains of the species Paraburkholderia zhejiangensis proposed to be transferred to this genus were also isolated from human clinical samples (Vandamme et al, 2013).…”

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confidence: 99%