<i>Burkholderia</i> gut symbiont induces insect host fecundity by modulating <i>Kr‐h1</i> gene expression

Lee, Junbeom; Lee, Dae‐Weon

doi:10.1002/arch.21987

Cited by 2 publications

(1 citation statement)

References 65 publications

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“…While there may be immune processes that we cannot capture through gene expression analyses (i.e., changes in the number of immune cells), we hypothesize that the rapid clearance of S. marcescens observed in symbiotic individuals is a byproduct of other changes in the host elicited by the symbiosis, or of indirect bacterial interactions within the host, rather than a result of A. tristis mounting a direct immune response upon S. marcescens infection. Symbiosis with Caballeronia has been shown to result in diverse benefits across many insect species (Kim et al, 2015;Kaltenpoth and Floŕez, 2020;Hunter et al, 2022;Lee and Lee, 2023). Such changes could influence the outcome of phytopathogen infection, even when not directly tied to host immunity.…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression in the insect vector Anasa tristis in response to symbiont colonization but not infection with a vectored phytopathogen

Mendiola,

Chen,

Lukubye

et al. 2024

Front. Ecol. Evol.

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Many insects selectively associate with specific microbes in long-term, symbiotic relationships. Maintaining these associations can be vital for the insect hosts’ development, but insects must also contend with potential coinfections from other microbes in the environment. Fending off microbial threats while maintaining mutualistic microbes has resulted in many insects developing specialized symbiotic organs to house beneficial microbes. Though locally concentrated in these organs, symbiont establishment can have global consequences for the insect, including influence over the success of coinfecting microbes in colonizing the insect host. We use a transcriptomic approach to examine how the mutualistic symbiosis between the agricultural pest Anasa tristis and bacteria in the genus Caballeronia affects insect gene expression locally within the symbiotic organs and in the insect host at large. We simultaneously determine whether Caballeronia colonization impacts insect host responses to infection with the plant pathogen Serratia marcescens, which it vectors to plants. We found that no significant differential gene expression was elicited by infection with S. marcescens. This was a surprising finding given previous work indicating that symbiotic A. tristis clear S. marcescens infection rapidly compared to aposymbiotic individuals. Our results indicate that symbiotic and nonsymbiotic tissues in A. tristis differ greatly in their gene expression, particularly following successful symbiont colonization. We found evidence for local downregulation of host immunity and upregulation of cell communication within the symbiotic organs, functions which can facilitate the success of the A. tristis-Caballeronia symbiosis.

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Section: Discussionmentioning

confidence: 99%

Differential gene expression in the insect vector Anasa tristis in response to symbiont colonization but not infection with a vectored phytopathogen

Mendiola,

Chen,

Lukubye

et al. 2024

Front. Ecol. Evol.

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Alteration of lipopolysaccharide O antigen leads to avirulence of gut-colonizing Serratia marcescens

Lee,

Kim,

Lee

et al. 2023

Front. Microbiol.

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The reason why the potent entomopathogen Serratia marcescens fails to kill insects through oral infection is unknown. To compare effects of septic injection and oral administration of S. marcescens, we used a model bean bug, Riptortus pedestris. Most R. pedestris insects survived oral infections, but not septic infections. Although the number of S. marcescens cells in hemolymph after oral infection, which were originated from gut-colonizing S. marcescens, was higher than the fatal number of cells used in septic injection, they did not kill host insects, suggesting a loss of virulence in gut-colonizing S. marcescens cells. When gut-colonizing S. marcescens cells were septically injected into insects, they failed to kill R. pedestris and survive in hemolymph. To understand the avirulence mechanisms in gut-colonizing bacteria, lipopolysaccharides of S. marcescens were analyzed and revealed that the O antigen was lost during gut colonization. Gut-colonizing S. marcescens cells were resistant to humoral immune responses but susceptible to cellular immune responses, easily succumbing to phagocytosis of hemocytes. When cellular immunity was suppressed, the gut-colonizing S. marcescens cells recovered their virulence and killed insects through septic injection. These results suggest that a key mechanism of avirulence in orally infected S. marcescens is the loss of the O antigen, resulting in susceptibility to host’s cellular immune responses.

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Burkholderia gut symbiont induces insect host fecundity by modulating Kr‐h1 gene expression

Cited by 2 publications

References 65 publications

Differential gene expression in the insect vector Anasa tristis in response to symbiont colonization but not infection with a vectored phytopathogen

Differential gene expression in the insect vector Anasa tristis in response to symbiont colonization but not infection with a vectored phytopathogen

Alteration of lipopolysaccharide O antigen leads to avirulence of gut-colonizing Serratia marcescens

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