<i>C. elegans</i> PEZO-1 is a Mechanosensitive Ion Channel Involved in Food Sensation

Millet, Jonathan R.M.; Romero, Luis O.; Lee, Jungsoo; Bell, Briar; Vásquez, Valeria

doi:10.1101/2021.05.10.443141

Cited by 3 publications

(4 citation statements)

References 96 publications

(146 reference statements)

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“…We observed pezo-1 expression in several head neurons, pharynx-intestinal valve, vulval muscle, spermatheca, CAN, and male tail neurons. Male-specific neurons that showed strong pezo-1 expression included PCB, PVV, and several sensory ray neurons (Figure 1G-K, see also Millet et al (2021) 27 ). PCB was previously shown to be required for vulva recognition 40 .…”

Section: Pezo-1 and Trp-4 Are Expressed In Male-specific Neuronsmentioning

confidence: 66%

“…Mutations in pezo-1 were previously shown to cause feeding-related phenotypes in C. elegans 27,28 . To test whether trp-4 and pezo-1 mutants showed signs of starvation, we measured their body size.…”

Section: Methodsmentioning

confidence: 99%

“…Like other invertebrates, C. elegans has a single piezo protein, PEZO-1. In the hermaphrodite, PEZO-1 has been shown to have roles in ovulation, defecation, and food intake 11,[26][27][28] .…”

Section: Introductionmentioning

confidence: 99%

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PEZO-1 and TRP-4 mechanosensors are involved in mating behavior inCaenorhabditis elegans

Brugman

Susoy

Whittaker

et al. 2022

Preprint

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Male mating in Caenorhabditis elegans is a complex behavior with a strong mechanosensory component. C. elegans has several characterized mechanotransducer proteins, but few have been shown to contribute to mating. Here, we investigated the roles of PEZO-1, a piezo channel, and TRP-4, a mechanotransducing TRPN channel, in male mating behavior. We show that pezo-1 is expressed in several male-specific neurons with known roles in mating. We show that, among other neurons, trp-4 is expressed in the PCA sensory neuron, which monitors relative sliding between the male and the hermaphrodite and inhibits neurons involved in vulva detection. Mutations in both genes compromise many steps of mating, including initial response to the hermaphrodite, scanning, turning, and vulva detection. We performed pan-neuronal imaging during mating between freely-moving mutant males and hermaphrodites. Both pezo-1 and trp-4 mutants showed spurious activation of the sensory neurons involved in vulva detection. In trp-4 mutants, this spurious activation might be caused by PCA failure to inhibit vulva-detecting neurons during scanning. Indeed, we show that without functional TRP-4, PCA fails to detect the relative sliding between the male and hermaphrodite. Cell-specific TRP-4 expression restores PCA's mechanosensory function. Our results demonstrate new roles for both PEZO-1 and TRP-4 mechanotransducers in C. elegans mating behavior.

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Section: Pezo-1 and Trp-4 Are Expressed In Male-specific Neuronsmentioning

confidence: 66%

Section: Methodsmentioning

confidence: 99%

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PEZO-1 and TRP-4 mechanosensors are involved in mating behavior inCaenorhabditis elegans

Brugman

Susoy

Whittaker

et al. 2022

Preprint

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“…The TRP-4 channel contributes to stretch-sensing by the DVA proprioceptor neurons 111 and PEZO-1 regulates feeding and reproduction and has isoforms that are expressed in ventral cord motor neurons. [119][120][121] Other candidate components to consider are putative mechanosensitive transcription factors. YAP/TAZ transcription factors are well known to be regulated by mechanical cues at the level of nuclear/cytoplasmic localization.…”

Section: Identifying Mechanisms For Feedback Regulationmentioning

confidence: 99%

Reciprocal interactions between transforming growth factor beta signaling and collagens: Insights from Caenorhabditis elegans

Goodman

Savage-Dunn

2021

Developmental Dynamics

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Studies in genetically tractable organisms such as the nematode Caenorhabditis elegans have led to pioneering insights into conserved developmental regulatory mechanisms. For example, Smad signal transducers for the transforming growth factor beta (TGF‐β) superfamily were first identified in C. elegans and in the fruit fly Drosophila. Recent studies of TGF‐β signaling and the extracellular matrix (ECM) in C. elegans have forged unexpected links between signaling and the ECM, yielding novel insights into the reciprocal interactions that occur across tissues and spatial scales, and potentially providing new opportunities for the study of biomechanical regulation of gene expression.

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Distinct mechanoreceptor pezo-1 isoforms modulate food intake in the nematode Caenorhabditis elegans

Hughes

Shah

Bai

et al. 2021

Preprint

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Two PIEZO mechanosensitive cation channels, PIEZO1 and PIEZO2, have been identified in mammals, where they are involved in numerous sensory processes. While structurally similar, PIEZO channels are expressed in distinct tissues and exhibit unique properties. How different PIEZOs transduce force, how their transduction mechanism varies, and how their unique properties match the functional needs of the distinct tissues where they are expressed remain all-important unanswered questions. The nematode Caenorhabditis elegans has a single PIEZO ortholog (pezo-1) predicted to have twelve isoforms. These isoforms share many transmembrane domains, but differ in those that distinguish PIEZO1 and PIEZO2 in mammals. Here we use translational and transcriptional reporters to show that long pezo-1 isoforms are selectively expressed in mesodermally derived tissues (such as muscle and glands). In contrast, shorter pezo-1 isoforms are primarily expressed in neurons. In the digestive system, different pezo-1 isoforms appear to be expressed in different cells of the same organ. We show that pharyngeal muscles, glands, and valve rely on long pezo-1 isoforms to respond appropriately to the presence of food. The unique pattern of complementary expression of pezo-1 isoforms suggest that different isoforms possess distinct functions. The number of pezo-1 isoforms in C. elegans, their differential pattern of expression, and their roles in experimentally tractable processes make this an attractive system to investigate the molecular basis for functional differences between members of the PIEZO family of mechanoreceptors.

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C. elegans PEZO-1 is a Mechanosensitive Ion Channel Involved in Food Sensation

Cited by 3 publications

References 96 publications

PEZO-1 and TRP-4 mechanosensors are involved in mating behavior inCaenorhabditis elegans

PEZO-1 and TRP-4 mechanosensors are involved in mating behavior inCaenorhabditis elegans

Reciprocal interactions between transforming growth factor beta signaling and collagens: Insights from Caenorhabditis elegans

Distinct mechanoreceptor pezo-1 isoforms modulate food intake in the nematode Caenorhabditis elegans

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