Campylobacter jejuniisolated from retail poultry meat, bovine feces and bile, and human diarrheal samples in Japan: Comparison of serotypes and genotypes

Abstract

To determine the significance of poultry and bovine as infectious sources of Campylobacter jejuni in Japan, the serotype distribution and pulsed-field gel electrophoresis (PFGE) patterns of poultry and bovine isolates were compared with those of isolates from patients with diarrhea in Akita (Japan). Serotypes O:2 and O:4-complex were common in human, poultry, and bovine isolates, and serotype O:23,36,53 was common in human and bovine isolates. SmaI PFGE patterns of isolates belonging to these serotypes were generated. Eight PFGE patterns were shared by poultry and human isolates and three patterns were shared by human and bovine isolates. Further analysis of the isolates having the same SmaI PFGE pattern by KpnI PFGE confirmed that four patterns and two patterns were still shared by poultry and human isolates, and bovine and human isolates, respectively. Thus, serotypic and genotypic data indicated a possible link between sporadic human campylobacteriosis and C. jejuni from retail poultry and bovine bile and feces, suggesting that bovine serves as an infectious source of C. jejuni in Japan, as is observed in other countries.

Campylobacter jejuniisolated from human cases in Chile showed indistinguishable pulsed field gel electrophoresis profiles with strains isolated from poultry and bovine sources

“…The PFGE profile analysis of the isolates with the enzymes KpnI confirmed that four patterns were shared by broiler and human isolates, being our result very similar to that obtained by Saito et al (2005) in Japan, where of 108 strains of C. jejuni, 4 PFGE KpnI profiles were also shared by poultry and human isolates.…”

“…*Corresponding author. Email: gighein@yahoo.es Studies in Denmark, Japan, and Finland provided substantial evidence for a genotypic link between C. jejuni isolates from humans with campylobacteriosis and those from poultry and bovine, by analyzing the PFGE patterns of the isolates (Ka¨renlampi et al 2011;Nakari, Hakkinen, & Siitonen, 2011;On, Nielsen, Engberg, & Madsen, 1998;Saito et al, 2005). A recent genotypic study by random amplification of polymorphic DNA polymerase chain reaction revealed that free range poultry genotypes were not found in human samples and that other reservoirs must be studied in Chile (Rivera et al, 2011).…”

Low Prevalence of Campylobacteriosis in the Northern Region of India

“…The prevalence of a particular serotype differs across countries based on their geographic locations. In Japan, the most predominant serotypes are B, D, and L, while those in Denmark are serotypes B, A, and D [24] [25]. Ishihara et al [26] identified 18 serotypes with major serotypes being B, D and R among the C. jejuni isolates from humans.…”

Detecção de fatores de virulência em estirpes de Campylobacter spp. isoladas de carcaças de suínos abatidos em frigoríficos

“…Atribui-se a transmissão para o ser humano ao contato direto com animais portadores, ao consumo de água e alimentos de origem animal e vegetal contaminados, carnes de aves, suínos ou bovinos malprocessadas, e à ingestão de leite não pasteurizado (Kumar et al, 2001;Saito et al, 2005;Scarcelli et al, 2005;Peyrat et al, 2008).…”