2004
DOI: 10.1128/aac.48.7.2483-2489.2004
|View full text |Cite
|
Sign up to set email alerts
|

Candida glabrata erg1 Mutant with Increased Sensitivity to Azoles and to Low Oxygen Tension

Abstract: A Candida glabrata erg1 (Cgerg1) mutant, CgTn201S, was identified by transposon mutagenesis and by increased fluconazole susceptibility. CgERG1 encodes a 489-amino-acid protein which, on the basis of its homology with Saccharomyces cerevisiae ERG1, is a squalene epoxidase essential for ergosterol synthesis. Interruption following codon 475 of CgErg1p decreased the ergosterol content by 50%; caused accumulation of the squalene precursor; increased the levels of susceptibility to fluconazole, itraconazole, and t… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

5
41
1

Year Published

2006
2006
2019
2019

Publication Types

Select...
5
3

Relationship

1
7

Authors

Journals

citations
Cited by 53 publications
(47 citation statements)
references
References 19 publications
5
41
1
Order By: Relevance
“…These results compare well to those obtained for the TERBINAFINE-SENSITIVE SQUALENE EPOXIDASE 281 recently described mutant carrying the erg1-1 allele, which encodes an SE variant with reduced enzymatic activity and which confers terbinafine sensitivity (8). In addition, the decrease in ergosterol content caused by the interruption of the Candida glabrata ERG1 gene also increased the sensitivity of this organism to terbinafine and azoles (35). Similar effects were observed in Candida albicans when ergosterol levels were reduced by the limited expression of SE (22), but this mutant also became more susceptible to other inhibitors not involved in sterol synthesis due to reduced drug efflux activity.…”
Section: Discussionsupporting
confidence: 77%
“…These results compare well to those obtained for the TERBINAFINE-SENSITIVE SQUALENE EPOXIDASE 281 recently described mutant carrying the erg1-1 allele, which encodes an SE variant with reduced enzymatic activity and which confers terbinafine sensitivity (8). In addition, the decrease in ergosterol content caused by the interruption of the Candida glabrata ERG1 gene also increased the sensitivity of this organism to terbinafine and azoles (35). Similar effects were observed in Candida albicans when ergosterol levels were reduced by the limited expression of SE (22), but this mutant also became more susceptible to other inhibitors not involved in sterol synthesis due to reduced drug efflux activity.…”
Section: Discussionsupporting
confidence: 77%
“…Previous work suggests that C. albicans, unlike the two species mentioned above, depends solely on the synthesis of endogenous ergosterol. No mechanism of sterol uptake was identified in previous work (27,31,32). Unlike S. cerevisiae and C. glabrata, there is lack of response to exogenous sterols by C. albicans wild-type, CaHEM1-null mutant, or sterol auxotrophs (27).…”
mentioning
confidence: 94%
“…A fosmid genomic library of Cg13928, a fluconazole-resistant clinical isolate, was constructed in copy control fosmid vector pCC1URA3 as described in Tsai et al (36). The average insert size of the genomic library was about 40 kb.…”
Section: Methodsmentioning
confidence: 99%
“…The Cgpdr1 mutant, CgTn173S, was generated by transposon mutagenesis using the custom transposon Tn5ϽCm URA3Ͼ, as described by Tsai et al (36). The ura3 mutants were selected on a MIN plus uracil agar plate containing 0.1% 5-fluoroorotic acid (FOA) (Lancaster, Pelham, NH).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation