BACKGROUND:With the changing health scenario fungal infections have increased significantly, contributing to morbidity, mortality and health care cost. Candida is major human fungal pathogens that cause both superficial and deep tissue infections. With emergence of nonalbicans Candida species, availability of advanced identification methods and antifungal resistance, the spectrum of candidiasis has changed. OBJECTIVE: The aim of our study was to identify the distribution of Candida species among clinical isolates, risk factors associated with candidiasis and their sensitivity pattern for common antifungal drugs. MATERIALS AND METHODS: One hundred thirty nine different clinical isolates of Candida were collected from indoor patients of a tertiary care centre of Gujarat from May 2009 to June 2010. Identification of Candida species and antifungal susceptibility testing was performed with miniAPI (Analytical Prophylactic Index) (Biomerieux, France) which is an automatic identification and susceptibility testing instrument. RESULTS: We found that the non-albicans Candida were more prevalent than Candida albicans. Candida tropicalis (48.9%) was the most common Candida spp. and also more resistant than that of C.albicans. C.albicans showed resistance against fluconazole (3.5%) and itraconazole (8.8%) whereas C.tropicalis were resistant to amphotericin B (10.3%), fluconazole (20.7%), itraconazole (32.3%), and voriconazole (23.5%) and flucytosine (5.8%). Overall resistance rates of Candida for amphotericin B, fluconazole, itraconazole, and voriconazole and flucytosine were 6.4%, 15.2%, 22.3%, 12.9%, 5% respectively. CONCLUSION: To achieve better clinical results species-level identification of Candida spp. and their antifungal sensitivity testing should be performed.