2021
DOI: 10.1101/2021.12.10.472193
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Candidatus Nitrosopolaris, a genus of putative ammonia-oxidizing archaea with a polar/alpine distribution

Abstract: Ammonia-oxidizing archaea (AOA) are key players in the nitrogen cycle. Here, we report four novel metagenome-assembled genomes (MAGs) assigned to the genus “UBA10452”, an uncultured lineage of putative AOA in the family Nitrososphaeraceae. Analysis of other eight previously reported MAGs and publicly available amplicon sequencing data revealed that the UBA10452 lineage is predominantly found in acidic polar and alpine soils. We propose a novel Candidatus genus, Ca. Nitrosopolaris, with four species representin… Show more

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Cited by 4 publications
(4 citation statements)
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References 92 publications
(211 reference statements)
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“…Archaea, which represented only 0.1% of the transcripts, consisted mostly of Thaumarchaea in the mineral layer as previously observed (Pessi et al. 2022b , Lu et al. 2017 , Shao et al.…”
Section: Discussionsupporting
confidence: 81%
“…Archaea, which represented only 0.1% of the transcripts, consisted mostly of Thaumarchaea in the mineral layer as previously observed (Pessi et al. 2022b , Lu et al. 2017 , Shao et al.…”
Section: Discussionsupporting
confidence: 81%
“…These phyla, excluding Planctomycetes, were also among the most abundant in the metagenomics dataset from these samples (Pessi et al ., 2021a). Archaea, which represented only 0.1% of the transcripts, consisted mostly of Thaumarchaea in the mineral layer as previously observed (Lu et al ., 2017; Shao et al ., 2019; Pessi et al ., 2021b). Communities in both the organic and mineral layers and across all vegetation types were dominated by aerobic acidophilic genera that play a role in the degradation of plant organic matter, including Acidothermus, Ca .…”
Section: Discussionmentioning
confidence: 99%
“…For each individual and co-assembly, we used anvi'o v7.0 [50] to bin contigs ≥2500 bp into metagenome-assembled genomes (MAGs) as previously described [37,38]. In brief, we used Prodigal v2.6.3 [51] to find gene calls, HMMER v. 3.3 [52] to identify a set of 71 bacterial and 76 archaeal single-copy genes [53], and DIAMOND v0.9.14 [54] to assign taxonomy to the singlecopy genes according to the Genome Taxonomy Database (GTDB) release 04-RS89 [55].…”
Section: Metagenome Binningmentioning
confidence: 99%
“…Twelve other low-quality genomes obtained by a metagenome-like assembling approach of non-axenic strains are also available [34]. Genome-resolved metagenomics has been established in recent years as a powerful approach to obtain microbial genomes, as it circumvents the difficulties associated with culturing microorganisms by reconstructing microbial genomes directly from environmental DNA [35][36][37][38]. Several genomes of uncultured polar Cyanobacteria have been obtained recently using this approach, including several novel lineages of early branching Cyanobacteria in the order Gloeobacterales [39][40][41].…”
Section: Introductionmentioning
confidence: 99%