‘<i>Candidatus Phytoplasma</i>trifolii’ associated with little leaf and witches’ broom disease of<i>Datura stramonium</i>L. in India

Singh, Namita; Madhupriya,; Rao, G. P.; Upadhayaya, P.P.

doi:10.5958/j.2249-4669.2.2.011

Cited by 13 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In India, 16SrVI group of phytoplasmas was earlier reported to be associated with several diseases of plants, viz. Araucaria little leaf (Gupta et al 2009), brinjal little leaf (Azadvar and Baranwal 2012), Withania little leaf (Zaim and Samad 1995; Samad et al 2006), Portulaca little leaf (Samad et al 2008), Datura little leaf (Raj et al 2009; Singh et al 2012), Croton leaf yellows (Madhupriya et al 2016), and Calotropis gigantea leaf yellows (Madhupriya et al 2010). However, 16SrVI-D subgroup of phytoplasma was only reported with brinjal little leaf (Azadvar and Baranwal 2012) and Catharanthus roseus little leaf (Bertaccini 2015).…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of phytoplasmas of ‘Clover proliferation’ group associated with three ornamental plant species in India

et al. 2016

View full text Add to dashboard Cite

Suspected phytoplasma symptoms of little leaf, yellowing, chlorosis, phyllody, witches’ broom, and stunting were observed on ten different ornamental plant species at New Delhi, Andhra Pradesh, Haryana, Bengaluru, and Pune, India, during March to July 2016. To investigate the possibility of phytoplasma etiology, PCR assays were performed using universal primer pairs (P1/P7 followed by 3Far/3Rev) specific to the phytoplasma 16Sr RNA gene. First round PCR amplification with primer pair P1/P7 did not yield expected 1.8 kb product of 16S rRNA region from any of the 17 symptomatic samples. However, 1.3 Kb amplicons were observed in nested PCR assays with 3Far/3Rev primer pair in symptomatic leaf samples of Hibiscus rosa-sinensis L. (Pune isolate), Saponaria officinalis L. (Pune isolate), and Allamanda cathartica L. (Delhi isolate). No amplifications were observed in any of the other tested symptomatic and non-symptomatic plant samples either in first round or second round of nested PCR assays with phytoplasma specific primer pairs. Pairwise sequence comparison of 16S rDNA sequences of the five positive phytoplasma strains of A. catharica, H. rosa-sinensis, and S. officinalis in the present study revealed 99–100% sequence identities with strains of ‘clover proliferation’ (16SrVI) group. Phylogenetic and virtual RFLP analysis of 16S rDNA sequences of the five identified phytoplasma strains belonging to three ornamental species further confirmed their clustering and grouping with member strains of ‘clover proliferation’ subgroup D. This is the first record of the phytoplasma association of ‘clover proliferation’ subgroup D with H. rosa-sinensis, S. officinalis, and A. cathartica in the world.

show abstract

Section: Resultsmentioning

confidence: 99%

Molecular characterization of phytoplasmas of ‘Clover proliferation’ group associated with three ornamental plant species in India

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In India, 16SrVI group of phytoplasmas has been reported to be associated with several diseases of plants, viz. Araucaria little leaf (Gupta et al 2009), brinjal little leaf (Azadvar and Baranwal 2012), Withania little leaf (Zaim and Samad 1995;Samad et al 2006), Portulaca little leaf (Samad et al 2008), Datura little leaf (Raj et al 2009;Singh et al 2012) and Calotropis gigantea leaf yellows (Madhupriya et al 2010). However, occurrence of 16SrVI-D subgroup phytoplasma was reported only on anantmool, lemongrass, sesame, Hibiscus rosa-sinensis, Saponaria officinalis and Allamanda cathartica (Madhupriya et al 2014(Madhupriya et al , 2015Khasa et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Phytoplasma etiology of BLL disease in India has been confirmed on the basis of symptoms, electron microscopy and PCR assays (Varma et al 1975;Verma and Dubey 1978;Shantha and Lakshmanan 1984;Azadvar and Baranwal 2012). Datura stramonium was reported as natural weed host for BLL phytoplasma in India (Singh et al 2012) and the leafhopper Hishimonas phycitis was identified as putative vector (Bindra and Singh 1969;Azadvar and Baranwal 2012). No transmission assays were performed by earlier workers to confirm any of the leafhoppers as natural vector of BLL disease in India.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization, vector identification and sources of phytoplasmas associated with brinjal little leaf disease in India

2017

View full text Add to dashboard Cite

Brinjal little leaf (BLL) is a widespread disease of phytoplasma etiology in India that induces severe economic losses. Surveys were conducted in eight brinjal-growing states of India during July 2014 to September 2015 and eighteen BLL samples showing little leaf, phyllody and witches' broom symptoms were collected for phytoplasma identification. Presence of phytoplasmas was confirmed in all the eighteen BLL samples using polymerase chain reaction with phytoplasma-specific primer pairs (P1/P6, R16F2n/R16R2). Pair wise sequence comparison and phylogenetic relationship of 16S rRNA gene sequences of BLL phytoplasma strains confirmed that sixteen out of eighteen BLL strains belonged to clover proliferation phytoplasma (16SrVI) group and two BLL strains (GKP-A and GKP-B) from Gorakhpur, Uttar Pradesh, were classified under 16SrII group. Further virtual RFLP analysis of 16S rDNA sequences allowed finer classification of BLL strains into 16SrII-D and 16SrVI-D subgroups. BLL phytoplasma strains belonging to 16SrVI-D subgroup were found as the most widespread phytoplasma strains associated with BLL disease in India. 16SrVI-D subgroup phytoplasma association with two symptomatic weed species viz. Cannabis sativa subsp. sativa at Noida, Uttar Pradesh and Portulaca oleracea at IARI fields, New Delhi was also confirmed by nested PCR assays with similar set of phytoplasma-specific primers, pairwise 16S rDNA sequence comparison, phylogeny and virtual RFLP analysis. Out of five identified leafhopper species from BLL-infected fields at Noida, Uttar Pradesh and Delhi, only Hishimonas phycitis was identified as carrier and natural vector of 16SrVI-D subgroup of phytoplasmas by nested PCR assays, sequence comparison, phylogeny, virtual RFLP analysis and transmission assays.

show abstract

“…Furthermore, weed species Datura stramonium , D. inoxia , Cannabis sativa subsp. sativa , Portulaca oleracea and P. grandiflora were identified as alternate hosts for the phytoplasma [ 19 , 39 , 44 ] while leafhopper Hishimonas phycitis was predicted as the likely vectors for the disease transmission [ 4 , 17 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of little leaf disease incidence and resistance in eggplant

Karthikeyan,

Yogiraj,

Elaiyabharathi

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Background Little leaf disease caused by phytoplasma infection is a significant threat to eggplant (also known as brinjal) cultivation in India. This study focused on the molecular characterisation of the phytoplasma strains and insect vectors responsible for its transmission and screening of brinjal germplasm for resistance to little leaf disease. Results Surveys conducted across districts in the Tamil Nadu state of India during 2021–2022 showed a higher incidence of phytoplasma during the Zaid (March to June), followed by Kharif (June to November) and Rabi (November to March) seasons with mean incidence ranging from 22 to 27%. As the name indicates, phytoplasma infection results in little leaf (reduction in leaf size), excessive growth of axillary shoots, virescence, phyllody, stunted growth, leaf chlorosis and witches’ broom symptoms. PCR amplification with phytoplasma-specific primers confirmed the presence of this pathogen in all symptomatic brinjal plants and in Hishimonus phycitis (leafhopper), providing valuable insights into the role of leafhoppers in disease transmission. BLAST search and phylogenetic analysis revealed the phytoplasma strain as “Candidatus Phytoplasma trifolii”. Insect population and disease dynamics are highly influenced by environmental factors such as temperature, relative humidity and rainfall. Further, the evaluation of 22 eggplant accessions revealed immune to highly susceptible responses where over 50% of the entries were highly susceptible. Finally, additive main effect and multiplicative interaction (AMMI) and won-where biplot analyses identified G18 as a best-performing accession for little leaf resistance due to its consistent responses across multiple environments. Conclusions This research contributes essential information on little leaf incidence, symptoms, transmission and resistance profiles of different brinjal genotypes, which together ensure effective and sustainable management of this important disease of eggplants.

show abstract

‘Candidatus Phytoplasmatrifolii’ associated with little leaf and witches’ broom disease ofDatura stramoniumL. in India

Cited by 13 publications

References 0 publications

Molecular characterization of phytoplasmas of ‘Clover proliferation’ group associated with three ornamental plant species in India

Molecular characterization of phytoplasmas of ‘Clover proliferation’ group associated with three ornamental plant species in India

Molecular characterization, vector identification and sources of phytoplasmas associated with brinjal little leaf disease in India

Comprehensive analysis of little leaf disease incidence and resistance in eggplant

Contact Info

Product

Resources

About