<i>Cannabis</i>Systematics at the Levels of Family, Genus, and Species

McPartland, John M.

doi:10.1089/can.2018.0039

Cited by 219 publications

(200 citation statements)

References 39 publications

(43 reference statements)

Supporting

Mentioning

187

Contrasting

Unclassified

Order By: Relevance

“…Cannabis ruderalis contains the lowest concentrations of Δ9‐tetrahydrocannabidiol (THC) . European Cannabis sativa contains more cannabidiol (CBD) than THC, whereas Asian Cannabis indica has more THC than CBD …”

Section: Is Cannabis An Option For Epilepsy?mentioning

confidence: 99%

“…22 European Cannabis sativa contains more cannabidiol (CBD) than THC, whereas Asian Cannabis indica has more THC than CBD. 23 Although artisanal cannabis is considered a "miracle therapy," at present there are not regulations to maintain the quality and purity of the drug during the obtaining procedure. Artisanal cannabis oil may contain abiotic (dust, fertilizers) and biotic (ie, insect, fungi, bacteria) contaminants, heavy metals, pesticides, etc, 24 a situation that represents a high risk to the health of patients.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Is cannabidiol a drug acting on unconventional targets to control drug‐resistant epilepsy?

Rocha¹,

Frías‐Soria²,

Ortíz

et al. 2020

Epilepsia Open

View full text Add to dashboard Cite

Cannabis has been considered as a therapeutic strategy to control intractable epilepsy. Several cannabis components, especially cannabidiol (CBD), induce antiseizure effects. However, additional information is necessary to identify the types of epilepsies that can be controlled by these components and the mechanisms involved in these effects. This review presents a summary of the discussion carried out during the 2nd Latin American Workshop on Neurobiology of Epilepsy entitled “Cannabinoid and epilepsy: myths and realities.” This event was carried out during the 10th Latin American Epilepsy Congress in San José de Costa Rica (September 28, 2018). The review focuses to discuss the use of CBD as a new therapeutic strategy to control drug‐resistant epilepsy. It also indicates the necessity to consider the evaluation of unconventional targets such as P‐glycoprotein, to explain the effects of CBD in drug‐resistant epilepsy.

show abstract

Section: Is Cannabis An Option For Epilepsy?mentioning

confidence: 99%

mentioning

confidence: 99%

Is cannabidiol a drug acting on unconventional targets to control drug‐resistant epilepsy?

Rocha¹,

Frías‐Soria²,

Ortíz

et al. 2020

Epilepsia Open

View full text Add to dashboard Cite

show abstract

“…Cannabis, an annual and dioecious member of the family Cannabaceae, is an economically important genus providing protein- and oil-rich seeds, fibre biomass for industrial (construction, textile and paper) utilization, and a wide variety of secondary metabolites, predominantly terpenes and cannabinoids (Lynch et al, 2016; McPartland, 2018; Onofri and Mandolino, 2017). Cannabis produces over 150 types of terpenes and ∼100 different cannabinoids (Hanuš et al, 2016; Booth and Bohlman, 2019), however, its categorization into drug type and fibre type has historically been based mainly on a single cannabinoid, Δ 9 -tetrahydrocannabinol (THC).…”

Section: Introductionmentioning

confidence: 99%

“…These deficiencies are due to limited genetic research, irregular breeding efforts, unorganized selection, ex situ conservation and government restrictions causing high heterozygosity in the cannabis genome (e.g. Rahn et al, 2016; McPartland 2018).…”

Section: Introductionmentioning

confidence: 99%

A Single Nucleotide Polymorphism assay sheds light on the extent and distribution of genetic diversity, population structure and functional basis of key traits in cultivated North American Cannabis

Henry¹,

Khatodia²,

Kapoor³

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundThe taxonomic classification of the Cannabis genus has been delineated through three main types: sativa (long and less branched plant with long and narrow leaves), indica (short but highly branched plant with broader leaves) and ruderalis (wild type with short stature, less branching and small thick leaves). While still under discussion, particularly whether the genus is polytypic or monotypic, this broad classification reflects putative geographical origin of each group and putative chemotypic and pharmacology.MethodsHere we describe a thorough investigation of cannabis accessions using a set of 22 highly informative and polymorphic SNP markers associated with important traits such as cannabinoid and terpenoid expression as well as fibre and resin production. The assay offers insight into cannabis population structure, phylogenetic relationship, population genetics and correlation to secondary metabolite concentrations and demonstrate the utility of this assay for rapid, repeatable and cost-efficient genotyping of commercial and industrial cannabis accessions for use in product traceability, breeding programs, regulatory compliance and consumer education.ResultsThe main outcomes are the identification of 5 clusters in the sample set available, including industrial hemp, resin hemp which likely underwent a bottleneck to stabilize CBDA accumulation (Type II & III). THC resin (type I) make up the other three clusters with terpinolene (colloquial “sativa” or “NLD”), myrcene/pinene and myrcene/limonene (colloquial “indica”, “BLD”), which also putatively harbour an active CBCAS.ConclusionThe functional basis of key traits is also discussed as recently enabled by the NCBI Cannabis sativa Annotation Release 100, allowing for hypothesis testing with regards to secondary metabolite production as well as other key traits of importance for adaptable and compliant large-scale seed production under the new US Domestic Hemp Production Program.

show abstract

“…A wealth of information pertaining to botany, phytochemistry, biology, and medicinal properties of Cannabis sativa has been accumulated over the years since the abolition of the legal use of cannabis in 1937 [3,4]. Recent phylogenetics studies have redefined the Cannabaceae family to now include not only Cannabis and Humulus (hop) but also eight genera formerly assigned to the Celtidaceae family: Celtis, Pteroceltis, Aphananthe, Chaetachme, Gironniera, Lozanella, Trema, and Parasponia [5]. Other closely related families are Moraceae and Urticaceae, which comprise Boehmeria nivea (Chinese grass).…”

Section: Introductionmentioning

confidence: 99%

Top-Down Proteomics of Medicinal Cannabis

et al. 2019

View full text Add to dashboard Cite

The revised legislation on medicinal cannabis has triggered a surge of research studies in this space. Yet, cannabis proteomics is lagging. In a previous study, we optimised the protein extraction of mature buds for bottom-up proteomics. In this follow-up study, we developed a top-down mass spectrometry (MS) proteomics strategy to identify intact denatured protein from cannabis apical buds. After testing different source-induced dissociation (SID), collision-induced dissociation (CID), higher-energy collisional dissociation (HCD), and electron transfer dissociation (ETD) parameters on infused known protein standards, we devised three LC-MS/MS methods for top-down sequencing of cannabis proteins. Different MS/MS modes produced distinct spectra, albeit greatly overlapping between SID, CID, and HCD. The number of fragments increased with the energy applied; however, this did not necessarily translate into greater sequence coverage. Some precursors were more amenable to fragmentation than others. Sequence coverage decreased as the mass of the protein increased. Combining all MS/MS data maximised amino acid (AA) sequence coverage, achieving 73% for myoglobin. In this experiment, most cannabis proteins were smaller than 30 kD. A total of 46 cannabis proteins were identified with 136 proteoforms bearing different post-translational modifications (PTMs), including the excision of N-terminal M, the N-terminal acetylation, methylation, and acetylation of K resides, and phosphorylation. Most identified proteins are involved in photosynthesis, translation, and ATP production. Only one protein belongs to the phytocannabinoid biosynthesis, olivetolic acid cyclase.

show abstract

CannabisSystematics at the Levels of Family, Genus, and Species

Cited by 219 publications

References 39 publications

Is cannabidiol a drug acting on unconventional targets to control drug‐resistant epilepsy?

Is cannabidiol a drug acting on unconventional targets to control drug‐resistant epilepsy?

A Single Nucleotide Polymorphism assay sheds light on the extent and distribution of genetic diversity, population structure and functional basis of key traits in cultivated North American Cannabis

Top-Down Proteomics of Medicinal Cannabis

Contact Info

Product

Resources

About