<i>CELF2</i> is a candidate prognostic and immunotherapy biomarker in triple‐negative breast cancer and lung squamous cell carcinoma: A pan‐cancer analysis

Wang, Libo; Лонг, Линг; Guo, Chunguang; Jiao, Dechao; Li, Lifeng; Zhao, Jie; Han, Xinwei; Sun, Yuling

doi:10.1111/jcmm.16791

Cited by 15 publications

(15 citation statements)

References 50 publications

(130 reference statements)

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“…High expression of CELF2 means a better prognosis in various tumors [29]. For example, CELF2 inhibits the progression of breast cancer by downregulating NFATc1 [30].…”

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

Deng

Zhou

Chen

et al. 2022

Preprint

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Background: Hepatocellular carcinoma (HCC) has ranked sixth most common cancer in number of malignancies in the worldwide. There is plenty of evidence indicated that circular RNAs (circRNAs) exert vitally important roles in the progression of many malignancies. Nevertheless, the molecular mechanism and role of hsa_circ_0006421 in HCC are still unclear. The aim of our research is to verify the molecular mechanism and effects of hsa_circ_0006421 in HCC.Methods: First, we collected 34 paired HCC tissues and paraneoplastic tissues surgically resected from patients and analyzed the expression of hsa_circ_0006421 in HCC tissues and its relationship with clinicopathological characteristics. Then, CCK8, colony formation, cell migration assay, transwell invasion assay and Annexin-V/PI staining were used to assess the effects of hsa_circ_0006421 on the growth, migration, invasion and apoptosis of HCC cells. Next, quantitative real-time PCR (qRT-PCR) and Western blots were used to detect the expression of miR-134-5p, CELF2 and hsa_circ_0006421. In the end, the targeting interactions of miR-134-5p and hsa_circ_0006421，CELF2 and miR-134-5p were explored by the dual-luciferase reporter assay.Results: Hsa_circ_0006421 was diminished in HCC tissues and its down-regulation was related to cirrhosis history. Knocking down hsa_circ_0006421 promoted HCC cells growth, migration, invasion and inhibited apoptosis, whereas overexpressing hsa_circ_0006421 had the opposite effects. Moreover, hsa_circ_0006421 served as the competing endogenous RNA of miR-134-5p. Subsequently, there was a reciprocal relationship between CELF2 and miR-134-5p. Hsa_circ_0006421 could positively regulate the protein level of CELF2 in HCC. Conclusion: hsa_circ_0006421 inhibits liver cancer by regulating miR-134-5p/CELF2 axis.

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“…High expression of CELF2 means a better prognosis in various tumors [29]. For example, CELF2 inhibits the progression of breast cancer by downregulating NFATc1 [30].…”

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

Deng

Zhou

Chen

et al. 2022

Preprint

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“…CELF2 belongs to the CUGBP Elav-like family and is an RBP 28 . High expression of CELF2 indicates improved prognosis in various tumors 29 . For example, CELF2 inhibits the progression of breast cancer by downregulating NFATc1 30 .…”

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

Zhou

Chen

et al. 2022

Preprint

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Background Hepatocellular carcinoma (HCC) ranks the sixth most common cancer in the number of malignancies worldwide. Accumulating evidence indicated that circular RNAs (circRNAs) play vital roles in the progression of several malignancies. Nonetheless, the molecular mechanism and role of hsa_circ_0006421 in HCC are yet unclear. The present study aimed to verify the molecular mechanism and effects of hsa_circ_0006421 in HCC. Methods A total of 34 paired HCC tissues and paraneoplastic tissues surgically resected from patients were collected, and the expression of hsa_circ_0006421 in HCC tissues and the correlation with clinicopathological characteristics were analyzed. Then, CCK-8, colony formation, cell migration assay, transwell invasion assay, and Annexin-V/PI staining were used to assess the effects of hsa_circ_0006421 on the growth, migration, invasion, and apoptosis of HCC cells. Next, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to detect the expression of miR-134-5p, CELF2, and hsa_circ_0006421. Finally, the targeting interactions of miR-134-5p and hsa_circ_0006421, CELF2 and miR-134-5p were explored using the dual-luciferase reporter assay. Results Hsa_circ_0006421 was diminished in HCC tissues, and its downregulation was related to cirrhosis history. Hsa_circ_0006421 knockdown promoted HCC cell growth, migration, and invasion and inhibited apoptosis, whereas overexpression had opposite effects. Moreover, hsa_circ_0006421 served as the competing endogenous RNA of miR-134-5p. Subsequently, a reciprocal correlation between CELF2 and miR-134-5p was established. Hsa_circ_0006421 positively regulated the protein level of CELF2 in HCC. Conclusion Hsa_circ_0006421 inhibits liver cancer by regulating miR-134-5p/CELF2 axis.

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“…The GSVA was a popular bioinformatics algorithm, which was extensively utilized in cancer-related studies. ( Liu et al, 2021a ; Liu et al, 2021b ; Liu et al, 2021c ; Wang et al, 2021 ; Zhang Y et al, 2021 ). GSVA was performed to examine different biological activities among the three ferroptosis mediation patterns using the “GSVA” R package.…”

Section: Methodsmentioning

confidence: 99%

Ferroptosis Mediation Patterns Reveal Novel Tool to Implicate Immunotherapy and Multi-Omics Characteristics in Bladder Cancer

Liu

Zhang

et al. 2022

Front. Cell Dev. Biol.

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Background: The regulatory role of ferroptosis in malignant tumours has been recently demonstrated. However, the potential roles of ferroptosis mediation patterns in bladder cancer remain elusive.Materials and Methods: The ferroptosis mediation patterns of 889 bladder cancer samples were comprehensively evaluated based on ferroptosis-related genes. The underlying correlations between these mediation patterns and multi-omic characteristics of bladder cancer were systematically analysed. The ferroptosis mediation patterns of individual samples were quantified by ferropscore using the principal component analysis algorithm. The typical ferroptosis-related genes with prognostic roles were further randomly validated using immunohistochemical staining, real-time polymerase chain reaction and western blotting.Results: Three different ferroptosis mediation patterns were identified. The abundance of infiltration of 23 immune cells was different among the three mediation patterns. The quantification of ferroptosis mediation patterns in individual samples served as a promising tool for predicting patient survival outcomes; immune cell infiltration abundance; tumour mutation burden; oncogenic mutation status and tumour grade, stage and molecular subtypes. Low ferropscore combined with high tumour mutation burden was associated with the best survival prognosis. Expressions of PD-L1 (p < 0.001), PD-1 (p = 0.002) and CTLA-4 (p = 0.003) were all significantly upregulated in the high ferropscore group. Low ferropscores also predicted good immunotherapy response for anti-CTLA4 strategy. The mRNA and protein levels of FADS2, a typical ferroptosis-related gene used in the study, were higher in bladder cancer cell lines than in controlled SV-HUC-1 cells. In addition, immunohistochemical staining revealed significantly higher expression levels of FADS2 in human bladder cancer tumour tissues than in normal tissues.Conclusion: This study identified three distinct ferroptosis mediation patterns in bladder cancer. Quantification of ferroptosis mediation patterns in individual samples may help to improve the understanding of multiomic characteristics and guide future immunotherapy responses to bladder cancer.

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CELF2 is a candidate prognostic and immunotherapy biomarker in triple‐negative breast cancer and lung squamous cell carcinoma: A pan‐cancer analysis

Cited by 15 publications

References 50 publications

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

Ferroptosis Mediation Patterns Reveal Novel Tool to Implicate Immunotherapy and Multi-Omics Characteristics in Bladder Cancer

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