<i>Chelidonium majus</i>crude extract induces activation of peripheral blood mononuclear cells and enhances their cytotoxic effect toward HeLa cells

Popović, Aleksandra; Deljanin, Milena; Popović, Suzana; Todorović, D. M.; Đjurdjević, Predrag; Matić, Sanja; Stanković, Milan; Avramovic, Dusko; Baskić, Dejan

doi:10.1080/09603123.2021.1897534

Cited by 5 publications

(2 citation statements)

References 41 publications

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“…Chelidonium majus L. (CM, Papaveraceae), or greater celandine, is an important plant in western phytotherapy and in traditional Chinese medicine (Zielińska et al, 2018). Crude extracts of CM, as well as purified compounds derived from it, exhibit a broad spectrum of biological activities (antiinflammatory, antimicrobial, antitumoral, analgesic, hepatoprotective and antioxidant) that support some of the traditional uses of CM plants (Colombo and Bosisio, 1996;Nawrot, 2017;Zielińska et al, 2018;Huang et al, 2019;Popovic et al, 2021;Krzyżek et al, 2021). However, herbal medicine also claims that this plant has several important properties which have not yet been scientifically studied (Colombo and Bosisio, 1996;Gilca et al, 2010;Zielińska et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Dose-Dependent Changes in the Levels of Oxidative Stress Biomarkers in the Muscle Tissue of Rainbow Trout (Oncorhynchus mykiss Walbaum) after In Vitro Treatment by Extracts Derived from Stalks and Roots of Great Celadine (Chelidonium majus L.)

Stefanowski¹

2022

Agrobiodivers Improv Nutr health life Qual

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Consistent with our previous studies, we continue to evaluate the antioxidant potential of representatives belonging to the Papaveraceae family collected from the northern part of Poland using a muscle tissue model of rainbow trout (Oncorhynchus mykiss Walbaum). Therefore, in the present study, oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), protein oxidative modification carbonyl derivative content, total antioxidant capacity (TAC)] were used to evaluate the antioxidant activity of stalk and root extracts of Chelidonium majus L. (CM) at doses of 5 mg.mL -1 , 2.5 mg.mL -1 , 1.25 mg.mL -1 , and 0.63 mg.mL -1 . Muscle tissue homogenates of rainbow trout were used in this study. Phosphate buffer was used as a positive control. After incubation of the mixture at 25 °C for 2 h with continuous mixing, samples were used for biochemical studies. Results of our study revealed that a dose of CM extracts of 0.63 mg.mL -1 showed the highest antioxidant activity in the muscle tissue of rainbow trout. The extracts derived mainly from the roots of CM collected from rural areas were effective in reducing the levels of oxidative stress biomarkers by reducing lipid peroxidation markers, which may suggest that the active substances such as alkaloids (chelidonine, sanguinarine, berberine), flavonoids, phenols in these plants can effectively protect the membrane structures in muscle cells of salmonids. We also observed statistically significant reductions in levels of both aldehydic and ketonic derivatives of oxidatively modified proteins in muscle tissue of rainbow trout after incubation with CM extracts at this dose compared to the controls. The comparison of these results shows that CM extracts can effectively inhibit protein damage by scavenging free radicals and/or activation of antioxidant defenses. The secondary metabolites of CM, i.e. polyphenols and alkaloids, are most likely responsible for this effect. Using doses of 5 mg.mL -1 , 2.5 mg.mL -1 , and 1.25 mg.mL -1 of both root and stalk extracts in vitro study, statistically significant increases in levels of TBARS and OMP were observed. Screening of species belonging to the family Papaveraceae for other biological activities, including antioxidant activity, is essential and may be effective in the search for preventive measures in the pathogenesis of diseases caused by oxidative stress in human and veterinary medicine.

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Section: Introductionmentioning

confidence: 99%

Dose-Dependent Changes in the Levels of Oxidative Stress Biomarkers in the Muscle Tissue of Rainbow Trout (Oncorhynchus mykiss Walbaum) after In Vitro Treatment by Extracts Derived from Stalks and Roots of Great Celadine (Chelidonium majus L.)

Stefanowski¹

2022

Agrobiodivers Improv Nutr health life Qual

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“…The extract itself was not toxic to the PBMNCs. These findings suggest the immunomodulatory effect of the C. majus extract (Popovic et al, 2022). In primary endometrial cancer cells a low to no cytotoxic effect was observed when treated with 4 main alkaloids from C. majus (Capistrano I et al, 2015) Chelidonine is one of the most studied alkaloids present in C. majus.…”

Section: Family: Papaveraceaementioning

confidence: 87%

Tap the sap – investigation of latex-bearing plants in the search of potential anticancer biopharmaceuticals

Mazur

Bałdysz²,

Warowicka³

et al. 2022

Front. Plant Sci.

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Latex-bearing plants have been in the research spotlight for the past couple of decades. Since ancient times their extracts have been used in folk medicine to treat various illnesses. Currently they serve as promising candidates for cancer treatment. Up to date there have been several in vitro and in vivo studies related to the topic of cytotoxicity and anticancer activity of extracts from latex-bearing plants towards various cell types. The number of clinical studies still remains scarce, however, over the years the number is systematically increasing. To the best of our knowledge, the scientific community is still lacking in a recent review summarizing the research on the topic of cytotoxicity and anticancer activity of latex-bearing plant extracts. Therefore, the aim of this paper is to review the current knowledge on in vitro and in vivo studies, which focus on the cytotoxicity and anticancer activities of latex-bearing plants. The vast majority of the studies are in vitro, however, the interest in this topic has resulted in the substantial growth of the number of in vivo studies, leading to a promising number of plant species whose latex can potentially be tested in clinical trials. The paper is divided into sections, each of them focuses on specific latex-bearing plant family representatives and their potential anticancer activity, which in some instances is comparable to that induced by commonly used therapeutics currently available on the market. The cytotoxic effect of the plant’s crude latex, its fractions or isolated compounds, is analyzed, along with a study of cell apoptosis, chromatin condensation, DNA damage, changes in gene regulation and morphology changes, which can be observed in cell post plant extract addition. The in vivo studies go beyond the molecular level by showing significant reduction of the tumor growth and volume in animal models. Additionally, we present data regarding plant-mediated biosynthesis of nanoparticles, which is regarded as a new branch in plant latex research. It is solely based on the green-synthesis approach, which presents an interesting alternative to chemical-based nanoparticle synthesis. We have analyzed the cytotoxic effect of these particles on cells. Data regarding the cytotoxicity of such particles raises their potential to be involved in the design of novel cancer therapies, which further underlines the significance of latex-bearing plants in biotechnology. Throughout the course of this review, we concluded that plant latex is a rich source of many compounds, which can be further investigated and applied in the design of anticancer pharmaceuticals. The molecules, to which this cytotoxic effect can be attributed, include alkaloids, flavonoids, tannins, terpenoids, proteases, nucleases and many novel compounds, which still remain to be characterized. They have been studied extensively in both in vitro and in vivo studies, which provide an excellent starting point for their rapid transfer to clinical studies in the near future. The comprehensive study of molecules from latex-bearing plants can result in finding a promising alternative to several pharmaceuticals on the market and help unravel the molecular mode of action of latex-based preparations.

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Medicinal plants show remarkable antiproliferative potential in human cancer cell lines

Nawaz

Arif

Jamal

et al. 2022

Bioscience, Biotechnology, and Biochemistry

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Molecules isolated and identified from plant origin are used to manufacture most chemotherapeutic drugs for cancer treatment. We assumed that these plant extracts contain prolific bioactive compounds with potent antiproliferative activities and could be effective against different human cancer cells. Ethanolic extracts were prepared from Chelidonium majus, Myrica cerifera, Fumaria indica, Nigella sativa, and Silybum marianum, and the antiproliferative assay was performed in HepG2 and HeLa human cancer cell lines. All plants extract exhibited antiproliferative potential against studied cancer cell lines in the dose and time-dependent manner. Chelidonium majus and Silybum marianum have shown promising results against HepG2 and HeLa cells, respectively, followed by Myrica cerifera, Fumaria indica, and Nigella sativa. Results indicated that utilization of whole plant extract as anticancer compounds could be of great value in generating novel chemotherapeutic drugs.

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Chelidonium majuscrude extract induces activation of peripheral blood mononuclear cells and enhances their cytotoxic effect toward HeLa cells

Cited by 5 publications

References 41 publications

Dose-Dependent Changes in the Levels of Oxidative Stress Biomarkers in the Muscle Tissue of Rainbow Trout (Oncorhynchus mykiss Walbaum) after In Vitro Treatment by Extracts Derived from Stalks and Roots of Great Celadine (Chelidonium majus L.)

Dose-Dependent Changes in the Levels of Oxidative Stress Biomarkers in the Muscle Tissue of Rainbow Trout (Oncorhynchus mykiss Walbaum) after In Vitro Treatment by Extracts Derived from Stalks and Roots of Great Celadine (Chelidonium majus L.)

Tap the sap – investigation of latex-bearing plants in the search of potential anticancer biopharmaceuticals

Medicinal plants show remarkable antiproliferative potential in human cancer cell lines

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