<i>Chlamydomonas</i>: Fast tracking from genomics

Findinier, Justin; Grossman, Arthur R.

doi:10.1111/jpy.13356

Cited by 6 publications

(4 citation statements)

References 111 publications

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“…For decades, the biflagellate microalga C. reinhardtii has been used as a model for specific biological processes, including, for example, photosynthesis, cilia formation and function, and light-driven processes [ 85 ]. The alga can be genetically manipulated, its genome is annotated, and many molecular tools and large-scale mutant libraries are available [ 86 , 87 , 88 , 89 ].…”

Section: Terrestrial and Freshwater Microalgaementioning

confidence: 99%

Exchange or Eliminate: The Secrets of Algal-Bacterial Relationships

Burgunter-Delamare,

Shetty,

Vuong

et al. 2024

Plants

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Algae and bacteria have co-occurred and coevolved in common habitats for hundreds of millions of years, fostering specific associations and interactions such as mutualism or antagonism. These interactions are shaped through exchanges of primary and secondary metabolites provided by one of the partners. Metabolites, such as N-sources or vitamins, can be beneficial to the partner and they may be assimilated through chemotaxis towards the partner producing these metabolites. Other metabolites, especially many natural products synthesized by bacteria, can act as toxins and damage or kill the partner. For instance, the green microalga Chlamydomonas reinhardtii establishes a mutualistic partnership with a Methylobacterium, in stark contrast to its antagonistic relationship with the toxin producing Pseudomonas protegens. In other cases, as with a coccolithophore haptophyte alga and a Phaeobacter bacterium, the same alga and bacterium can even be subject to both processes, depending on the secreted bacterial and algal metabolites. Some bacteria also influence algal morphology by producing specific metabolites and micronutrients, as is observed in some macroalgae. This review focuses on algal-bacterial interactions with micro- and macroalgal models from marine, freshwater, and terrestrial environments and summarizes the advances in the field. It also highlights the effects of temperature on these interactions as it is presently known.

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Section: Terrestrial and Freshwater Microalgaementioning

confidence: 99%

Exchange or Eliminate: The Secrets of Algal-Bacterial Relationships

Burgunter-Delamare,

Shetty,

Vuong

et al. 2024

Plants

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“…Although they encompass both prokaryotes (cyanobacteria) and eukaryotes (“true” microalgae), they will be collectively referred to as microalgae in the present review [ 6 ]. Microalgae such as the model Chlamydomonas reinhardtii are widely studied to improve our understanding of key plant functions such as chloroplast-based photosynthesis and nutrient assimilation, and the structure and function of the eukaryotic flagella [ 7 , 8 , 9 , 10 ]. In addition, due to their physiology and biochemistry, significant research is focusing on the development of microalgae-based environmental biotechnologies for food and high-value molecules production, biofuels generation, wastewater treatment and nutrient recovery [ 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Key Proteomics Tools for Fundamental and Applied Microalgal Research

Plouviez,

Dubreucq

2024

Proteomes

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Microscopic, photosynthetic prokaryotes and eukaryotes, collectively referred to as microalgae, are widely studied to improve our understanding of key metabolic pathways (e.g., photosynthesis) and for the development of biotechnological applications. Omics technologies, which are now common tools in biological research, have been shown to be critical in microalgal research. In the past decade, significant technological advancements have allowed omics technologies to become more affordable and efficient, with huge datasets being generated. In particular, where studies focused on a single or few proteins decades ago, it is now possible to study the whole proteome of a microalgae. The development of mass spectrometry-based methods has provided this leap forward with the high-throughput identification and quantification of proteins. This review specifically provides an overview of the use of proteomics in fundamental (e.g., photosynthesis) and applied (e.g., lipid production for biofuel) microalgal research, and presents future research directions in this field.

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“…In this study, we focused on activating CREs in the unicellular green alga Chlamydomonas reinhardtii. In the past 70 years, this species has been developed into a powerful and widely used model system to study processes such as photosynthesis and cell motility, and it has advanced our understanding of cell organelles like cilia and pyrenoid (Findinier & Grossman, 2023;Salom e & Merchant, 2019;Sasso et al, 2018). Efficient genetic tools including genome editing with CRISPR technology (Akella et al, 2021;Ferenczi et al, 2017;Shin et al, 2016), a large library of mapped mutants (Li et al, 2019), as well as a fully sequenced genome (Craig et al, 2022;Merchant et al, 2007), further enable the successful utilization of this alga for basic and applied research.…”

Section: Introductionmentioning

confidence: 99%

Characterization of activating cis‐regulatory elements from the histone genes of Chlamydomonas reinhardtii

Lihanova,

Nagel,

Jakob

et al. 2024

The Plant Journal

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SUMMARYRegulation of gene expression in eukaryotes is controlled by cis‐regulatory modules (CRMs). A major class of CRMs are enhancers which are composed of activating cis‐regulatory elements (CREs) responsible for upregulating transcription. To date, most enhancers and activating CREs have been studied in angiosperms; in contrast, our knowledge about these key regulators of gene expression in green algae is limited. In this study, we aimed at characterizing putative activating CREs/CRMs from the histone genes of the unicellular model alga Chlamydomonas reinhardtii. To test the activity of four candidates, reporter constructs consisting of a tetramerized CRE, an established promoter, and a gene for the mCerulean3 fluorescent protein were incorporated into the nuclear genome of C. reinhardtii, and their activity was quantified by flow cytometry. Two tested candidates, Eupstr and Ehist cons, significantly upregulated gene expression and were characterized in detail. Eupstr, which originates from highly expressed genes of C. reinhardtii, is an orientation‐independent CRE capable of activating both the RBCS2 and β2‐tubulin promoters. Ehist cons, which is a CRM from histone genes of angiosperms, upregulates the β2‐tubulin promoter in C. reinhardtii over a distance of at least 1.5 kb. The octamer motif present in Ehist cons was identified in C. reinhardtii and the related green algae Chlamydomonas incerta, Chlamydomonas schloesseri, and Edaphochlamys debaryana, demonstrating its high evolutionary conservation. The results of this investigation expand our knowledge about the regulation of gene expression in green algae. Furthermore, the characterized activating CREs/CRMs can be applied as valuable genetic tools.

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Chlamydomonas: Fast tracking from genomics

Cited by 6 publications

References 111 publications

Exchange or Eliminate: The Secrets of Algal-Bacterial Relationships

Exchange or Eliminate: The Secrets of Algal-Bacterial Relationships

Key Proteomics Tools for Fundamental and Applied Microalgal Research

Characterization of activating cis‐regulatory elements from the histone genes of Chlamydomonas reinhardtii

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