Cinnamomum camphora Leaves Alleviate Allergic Skin Inflammatory Responses In Vitro and In Vivo

Abstract

In this study, we investigated the therapeutic potential of Cinnamomum camphora leaves on allergic skin inflammation such as atopic dermatitis. We evaluated the effects of C. camphora leaves on human adult low-calcium high-temperature keratinocytes and atopic dermatitis mice. C. camphora leaves inhibited Macrophage-derived chemokine (an inflammatory chemokine) production in interferon-γ (10 ng/mL) stimulated Human adult low-calcium high-temperature keratinocytes in a dose dependent manner. C. camphora leaves suppressed the phosphorylation of janus kinase signal transducer and activator of transcription 1. C. camphora leaves also suppressed the phosphorylation of extracellular signal-regulated kinase 1/2, a central signaling molecule in the inflammation process. These results suggest that C. camphora leaves exhibits anti-inflammatory effect via the phosphorylation of signal transducer and activator of transcription 1 and extracellular signal-regulated kinase 1/2. To study the advanced effects of C. camphora leaves on atopic dermatitis, we induced experimental atopic dermatitis in mice by applying 2,4-dinitrochlorobenzene. The group treated with C. camphora leaves (100 mg/kg) showed remarkable improvement of atopic dermatitis symptoms: reduced serum immunoglobulin E levels, smaller lymph nodes with reduced thickness and length, decreased ear edema, and reduced levels of inflammatory cell infiltration in the ears. Interestingly, the effects of C. camphora leaves on atopic dermatitis symptoms were stronger than those of hydrocort cream, a positive control. Taken together, C. camphora leaves showed alleviating effects on the inflammatory chemokine production in vitro and atopic dermatitis symptoms in vivo . These results suggest that C. camphora leaves help in the treatment of allergic inflammation such as atopic dermatitis.

Anti-Inflammatory Property of the Essential Oil from Cinnamomum camphora (Linn.) Presl Leaves and the Evaluation of Its Underlying Mechanism by Using Metabolomics Analysis

“…In traditional Chinese medicine, the essential oil from this plant (EO) has been widely used to treat inflammation, rheumatic conditions, and muscular strains [ 2 ]. A growing number of pharmacological researches have shown that C. camphora exhibits a wide range of biological activities such as anti-inflammation, anti-bacteria, antioxidation, anti-fungi, and repellent properties [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. In our previous study [ 12 ], 48 constituents from EO were identified; linalool was the major compound, followed by eucalyptol, isoborneol, α-terpineol, and camphor ( Supplementary Material, Table S1 ).…”

Therapeutic Potential of Volatile Terpenes and Terpenoids from Forests for Inflammatory Diseases

“…Several sublines of NC mice including NC/Nga, NC/Jic, and NC/Tnd, have been developed [170]. Upon topical application of β-pinene, atopic-like skin conditions in NC/Tnd mice were ameliorated [171,172]. In the atopic dermatitis mouse model induced by 2,4-dinitrochlorobenzene, as well as in an in vitro experiment with a human adult low-calcium high-temperature keratinocytes, Cinnamomum camphora leaves, whose major component is camphor, alleviated atopic dermatitis symptoms and allergic skin inflammatory responses, such as inhibition of chemokine production as well as Jak/STAT and ERK 1/2 phosphorylation.…”

Optimization and Characterization of Microwave-Assisted Hydro-Distillation Extraction of Essential Oils from Cinnamomum camphora Leaf and Recovery of Polyphenols from Extract Fluid

“…The C. camphora is not only a high-quality resource of wood but also plays an important role in the utilization of fragrance, medicine, and chemical industry due to its special aromatic and medicinal value [ 1 , 2 , 3 , 4 ]. In addition, some studies indicated that the extract of C. camphora leaf and its bioactive components have antioxidant, anti-inflammatory, antialgal, antimicrobial, and insecticidal activities in vitro, as well as the effects of an anticonvulsant, neuroprotective, and alleviating atopic dermatitis in vivo [ 2 , 5 , 6 , 7 ].…”