<i>Clostridium difficile</i> Ileitis in Pediatric Inflammatory Bowel Disease

Butler, David; Torres-Torres, Sanet; Pahud, Barbara; Myers, Angela; Bass, Julie; Peter, Shawn D. St.; McCulloh, Russell J.

doi:10.1097/mpg.0000000000000737

Cited by 4 publications

(1 citation statement)

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“…This could be associated with two aspects caused by the syndrome: (1) The absence of food in the colon due to impaction of the cecum, and (2) the proliferation of microbial communities belonging to the family Clostridiaceae. In the case of microbial proliferation leading to digestive problems, this has been documented in broiler chickens [ 29 ] and children [ 30 ]. Cecal impaction promotes an environment for the proliferation of various pathogenic communities, and also causes food to not access the more distal sections of the gastrointestinal tract and so decreases the growth of resident communities, such as Ruminococcus spp.…”

Section: Discussionmentioning

confidence: 99%

Metataxonomic and Histopathological Study of Rabbit Epizootic Enteropathy in Mexico

Puón-Peláez

McEwan

Gómez-Soto

et al. 2020

Animals

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Epizootic rabbit enteropathy (ERE) affects young rabbits and represents 32% of the enteropathies in rabbit production farms in Mexico. The etiology of this syndrome has not been clarified yet. A metataxonomic and histopathology study of ERE was carried out to compare the gastrointestinal microbiota and histopathological lesions of healthy and positive-ERE rabbits. The metataxonomic study was done using an Illumina MiSeq (MiSeq® system, Illumina, San Diego California, USA) massive segmentation platform, and a Divisive Amplicon Denoising Algorithm 2 (DADA2 algorithm) was used to obtain Shannon and Simpson diversity indices as well as the relative abundance of the identified communities. For the histopathological study, paraffin sections of the cecum, ileo-cecal valve, and colon were stained with eosin and hematoxylin. AxioVision 4.9 software (Carl Zeiss MicroImaging GmbH, Jena, Germany) was used to measure the crypt depths. Statistical analysis was done using PERMANOVA analysis for the metataxonomic study and ANOVA for the histopathology study. Histopathologic analysis showed smaller sizes of crypts in the colon of ERE rabbits. Differences were observed in the diversity and abundance of the gastrointestinal microbiota between the analyzed groups. The genus Clostridium and the species Cloacibacillus porcorum and Akkermansia muciniphila were associated with ERE. The results obtained from this study can provide information for future clarification of the etiology and proposals of effective treatments.

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Section: Discussionmentioning

confidence: 99%