<i>Coccoloba alnifolia</i>Leaf Extract as a Potential Antioxidant Molecule Using In Vitro and In Vivo Assays

Melo, Luciana Fentanes Moura de; Gomes, Dayanne Lopes; Silva, Lucas Felipe da; Silva, Larissa Marina Pereira; Machado, Marina Lopes; Cadavid, Cesar Orlando Muñoz; Zucolotto, Silvana Maria; Oliveira, Riva de Paula; Santos, Déborah Yara Alves Cursino dos; Rocha, Hugo Alexandre Oliveira; Scortecci, Kátia Castanho

doi:10.1155/2020/3928706

Cited by 4 publications

(7 citation statements)

References 47 publications

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“…For the superoxide radical scavenging assay, activity was observed for both partition phases in a similar manner, higher than 50% when compared to control (Figure 4d). An important activity observed in these two partition phases was the copper chelating activity (Figure 4e), which was not observed previously for AE [23]. In addition, for all these antioxidant assays assessed, BF was found to have the highest antioxidant activity.…”

Section: Ae Partition Phases Antioxidant Activity Characterizationsupporting

confidence: 54%

“…Numerous efforts have been made to identify the biomolecules from medicinal plants and to correlate them with their biological and pharmacological activities [35]. Melo et al [23], working with six C. alnifolia leaf extracts, observed an important antioxidant activity using in vitro and in vivo assays. In addition, TLC showed the presence of gallic acid, p-coumaric acid, and vitexin.…”

Section: Discussionmentioning

confidence: 99%

“…This species, commonly known as Cauaçu, thrives across four distinct Brazilian biomes: the Atlantic and Amazon Rainforests, the Caatinga, and the Cerrado. A previous study [23] observed that six extracts of its leaves were not cytotoxic against NIH/3T3 (murine fibroblast cell line) or tumor cells such as HeLa (human cervical adenocarcinoma cells), SiHa (human cervix squamous cell carcinoma), PC-3 (human prostatic adenocarcinoma cells), B16-F10 (murine melanoma cells), and PANC-1 (human pancreas epithelioid carcinoma cells). In addition, four extracts showed in vitro antioxidant activity in several tests.…”

Section: Introductionmentioning

confidence: 99%

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The Antioxidant and Immunomodulatory Potential of Coccoloba alnifolia Leaf Extracts

de Melo,

Barbosa,

Cordeiro

et al. 2023

IJMS

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Oxidative stress has been associated with different diseases, and different medicinal plants have been used to treat or prevent this condition. The leaf ethanolic extract (EE) and aqueous extract (AE) from Coccoloba alnifolia have previously been characterized to have antioxidant potential in vitro and in vivo. In this study, we worked with EE and AE and two partition phases, AF (ethyl acetate) and BF (butanol), from AE extract. These extracts and partition phases did not display cytotoxicity. The EE and AE reduced NO production and ROS in all three concentrations tested. Furthermore, it was observed that EE and AE at 500 μg/mL concentration were able to reduce phagocytic activity by 30 and 50%, respectively. A scratch assay using a fibroblast cell line (NHI/3T3) showed that extracts and fractions induced cell migration with 60% wound recovery within 24 h, especially for BF. It was also observed that AF and BF had antioxidant potential in all the assays evaluated. In addition, copper chelation was observed. This activity was previously not detected in AE. The HPLC-DAD analysis showed the presence of phenolic compounds such as p-cumaric acid and vitexin for extracts, while the GNPS annotated the presence of isoorientin, vitexin, kanakugiol, and tryptamine in the BF partition phase. The data presented here demonstrated that the EE, AE, AF, and BF of C. alnifolia have potential immunomodulatory effects, antioxidant effects, as well as in vitro wound healing characteristics, which are important for dynamic inflammation process control.

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Section: Ae Partition Phases Antioxidant Activity Characterizationsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Antioxidant and Immunomodulatory Potential of Coccoloba alnifolia Leaf Extracts

de Melo,

Barbosa,

Cordeiro

et al. 2023

IJMS

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“…Similar results were also observed when animals were treated with quercetin or Anacardium occidentale extracts [ 41 , 42 ]. The data suggest that these extracts may act synergistically with phenolic compounds to protect cells and C. elegans from oxidative stress by assisting in ROS reduction, consequently helping to maintain cellular homeostasis [ 28 , 38 , 39 , 42 – 44 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Activities of Commiphora leptophloeos (Mart.) J. B. Gillett) (Burseraceae) Leaf Extracts Using In Vitro and In Vivo Assays

Cordeiro

Ribeiro

Melo

et al. 2021

Oxidative Medicine and Cellular Longevity

Self Cite

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Commiphora leptophloeos is widely used in folk medicine without any scientific basis. Considering this, the aim of this study was to evaluate the chemical profile and the antioxidant activity of C. leptophloeos leaf extracts using in vitro and in vivo assays. Six extracts were obtained from fresh leaves using a serial extraction (nonpolar to polar solvents). These extracts were first evaluated with the presence of phytochemical compounds using the methods thin layer chromatography (TLC), ultrahigh performance liquid chromatography (UHPLC-DAD), and high performance liquid chromatography, both with diode array detection (HPLC-DAD). Based on the compounds identified, it was used some bioinformatics tools in order to identify possible pathway and gene targets. After that, the antioxidant capacity from these extracts was analysed by in vitro assays and in vivo assays using Caenorhabditis elegans model. Phytochemical analyses showed the presence of polyphenols, such as rutin, vitexin, and quercetin diglycosides in all extracts, especially in ethanol extract (EE) and methanol extract (EM). Bioinformatics analysis showed these polyphenols linked to antioxidant pathways. Furthermore, EE and EM displayed a high antioxidant capacity in DPPH and superoxide radical scavenging assays. They also had no effect on cell viability for 3T3 nontumour cell. However, for B16-F10 tumour cell lines, these extracts had toxicity effect. In vivo assays using C. elegans N2 showed that EE was not toxic, and it did not affect its viability nor its development. Besides, EE increased worm survival under oxidative stress and reduced intracellular reactive oxygen species (ROS) levels by 50%. Thus, C. leptophloeos EE displayed an important in vitro and in vivo antioxidant capacity. The EE extract has polyphenols, suggesting that these compounds may be responsible for a myriad of biological activities having this potential to be used in various biotechnological applications.

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“…2.7.3. High-Performance Liquid Chromatography with Diode Array Detection (CLAE-DAD) CLAE-DAD analysis was performed as described by [48,49]. An Agilent 1260 system equipped with a Zorbax C18 column (150 mm × 4.6 mm × 3.5 µm) at 45 • C was used.…”

Section: Quantification Of the Total Flavonoid Contentmentioning

confidence: 99%

Exploring the Antioxidant Potential of Talisia esculenta Using In Vitro and In Vivo Approaches

da Silva Cordeiro,

de Queiroz Aquino-Martins,

da Silva

et al. 2023

Nutrients

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Medicinal plants, such as Talisia esculenta, are rich in antioxidant biomolecules, which are used in the treatment and prevention of many diseases. The antioxidant potential of T. esculenta extracts obtained from leaves and fruit peels was investigated using biochemical and 3T3 cell line assays as well as in vivo assays using an organism model Tenebrio molitor. Four extracts were tested: hydroethanolic extracts from leaves (HF) and from fruit peels (HC), and infusion extracts from leaves (IF) and from fruit peels (IC). The biochemical assays demonstrated an antioxidant capacity verified by TAC, reducing power, DPPH, and copper chelating assays. None of the extracts exhibited cytotoxicity against 3T3 cells, instead offering a protection against CuSO4-induced oxidative stress. The antioxidant activity observed in the extracts, including their role as free radical scavengers, copper chelators, and stress protectors, was further confirmed by T. molitor assays. The CLAE-DAD analysis detected phenolic compounds, including gallic acid, rutin, and quercitrin, as the main constituents of the samples. This study highlights that leaf and fruit peels extracts of T. esculenta could be effective protectors against ROS and copper-induced stress in cellular and invertebrate models, and they should be considered as coadjutants in the treatment and prevention of diseases related to oxidative stress and for the development of natural nutraceutical products.

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Coccoloba alnifoliaLeaf Extract as a Potential Antioxidant Molecule Using In Vitro and In Vivo Assays

Cited by 4 publications

References 47 publications

The Antioxidant and Immunomodulatory Potential of Coccoloba alnifolia Leaf Extracts

The Antioxidant and Immunomodulatory Potential of Coccoloba alnifolia Leaf Extracts

Antioxidant Activities of Commiphora leptophloeos (Mart.) J. B. Gillett) (Burseraceae) Leaf Extracts Using In Vitro and In Vivo Assays

Exploring the Antioxidant Potential of Talisia esculenta Using In Vitro and In Vivo Approaches

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