CodY , ComA , DegU and Spo0A controlling lipopeptides biosynthesis in Bacillus amyloliquefaciens fmbJ

Abstract

Aim: In the study, we investigated the regulatory effects of these genes (codY, comA, degU and spo0A) on the biosynthesis of three lipopeptides (bacillomycin D, fengycin and surfactin) in Bacillus amyloliquefaciens. Methods and Results: The codY, comA, degU and spo0A genes in B. amyloliquefaciens fmbJ were knocked out. The results showed that the productions of bacillomycin D were significantly reduced compared with that of fmbJ. Their deletion induced great changes in the levels of transcripts specifying metabolic pathways, quorum sensing system and substance transport system in fmbJ. Moreover, overexpression of these genes improved the productions of bacillomycin D. In particular, the overexpression of spo0A enhanced bacillomycin D yield up to 648Á9 AE 60Á9 mg l À1 from 277Á3 AE 30Á5 mg l À1 . In addition, the yields of surfactin in fmbJDcodY and fmbJDdegU were significantly improved, and the regulatory factor CodY had no significant effect on the synthesis of fengycin. Conclusions: These genes positively regulated the expression of bacillomycin D and fengycin synthase genes in strain fmbJ. However, codY and degU negatively regulated surfactin biosynthesis. Moreover, it was found that CodY had a concentration dependence on bacillomycin D synthesis. Spo0A might play a direct regulatory role in the synthesis and secretion of bacillomycin D. Significance and Impact of the Study: This study indicated that genetic engineering of regulatory genes was an effective strategy to improve the yields of antimicrobial lipopeptides and provided promising strains for industrial production of lipopeptides.

iTRAQ-BASED Proteomic Analysis of the Mechanism of Fructose on Improving Fengycin Biosynthesis in Bacillus Amyloliquefaciens

“…These multifunctional enzymes catalyze ten amino acids into a cyclic decapeptide; then fatty acyl chain is attached to the peptide. Numerous genes, including codY , comA , degU and spo0A are also involved in regulating the biosynthesis of fengycin [ 14 ]. In previous works, B .amyloliquefaciens fmb-60 is able to enhance the yield of fengycin and upregulate the gene expression of fen in a modified Landy medium supplemented with fructose [ 15 ], but the regulatory mechanism of fructose on fengycin biosynthesis has not been elucidated yet.…”

Identification and functional analysis of non-coding regulatory small RNA FenSr3 in Bacillus amyloliquefaciens LPB-18

“…The thermo-sensitive knockout vectors pCBS were obtained from the Nanjing Agricultural University ( Sun et al, 2021 ). All primers used for this study are summarised in Table 2 .…”

“…Through transcriptome and proteome analysis, we previously explored the mechanism of the positive effects of fructose on fengycin biosynthesis ( Lu et al, 2021 ; Lu et al, 2022 ). Sun et al (2021) demonstrated that the biosynthesis of fengycin in B. amyloliquefaciens fmbJ was positively regulated by the genes codY , comA , and spo0A . The production of fengycin was enhanced by lecithin supplementation in the medium and increased the antagonism effect of B. amyloliquefaciens PPL against Cucumber mosaic virus (CMV) ( Kang, Park & Jung, 2021 ).…”

Expression of degQ gene and its effect on lipopeptide production as well as formation of secretory proteases in Bacillus subtilis strains

“…In addition, superordinated stimuli such as quorum sensing and nutrient availability influence lipopeptide biosynthesis. Specifically, several global regulators including Spo0A, AbrB, CodY, and DegU are involved in the control of NRPS expression (Nakano et al, 1992 ; Serror & Sonenshein, 1996 ; Sun et al, 2021 ; Vahidinasab et al, 2020 ).…”