<i>Coix lachryma-jobi</i> extract ameliorates inflammation and oxidative stress in a complete Freund’s adjuvant-induced rheumatoid arthritis model

Zhang, Chunfang; Zhang, Wenfeng; Shi, Rongyu; Tang, Bangyi; Xie, Shicheng

doi:10.1080/13880209.2019.1687526

Cited by 46 publications

(39 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2B), demonstrating that ICA could attenuate the iron deposit in LPS-induced synoviocytes. GPX, an antioxidant enzyme (35), was also detected in the present study. GPX levels were significantly reduced by LPS compared with the control group.…”

Section: Ica Attenuates Cell Death and Increases Cell Viability In Lpsupporting

confidence: 79%

Icariin enhances cell survival in lipopolysaccharide‑induced synoviocytes by suppressing ferroptosis via the Xc‑/GPX4 axis

Luo

Zhang

2020

Exp Ther Med

View full text Add to dashboard Cite

The mechanism of action of synovitis, as the vital pathological process of rheumatoid arthritis and osteoarthritis, remains to be elucidated. The effects and the mechanism of icariin (ICA), which is a promising therapeutic agent in synovitis, was investigated in the present study. In addition, ferroptosis, a vital cell process involved in several diseases, was also studied in synovitis for the first time. Lipopolysacc haride (LPS)-induced synoviocytes served as a synovitis cell model. The cells were divided into control, LPS and experimental groups and were treated with different concentrations of ICA. Cell viability was determined by Cell Counting Kit-8 assay and cell death was determined by flow cytometry. The expression levels of proteins (GPX4, SLC7A11, SLC3A2L, TRF, Nrf2 and NCOA4) were measured by western blotting. Quantification of malondialdehyde (MDA), iron and glutathione peroxidase 4 (GPX4) activity levels were performed via using corresponding assay kits. Cell death was increased, and cell viability was decreased in LPS-induced synoviocytes. Furthermore, MDA levels and iron content were elevated and GPX levels was reduced in LPS-induced synoviocytes. Transferrin receptor protein 1 and nuclear receptor coactivator 4 were upregulated and proteins of the Xc-/GPX4 axis, as well as nuclear factor erythroid 2-related factor 2, were decreased by LPS treatment. All aforementioned LPS affects were alleviated by ICA via a concentration-dependent manner. ICA counteracted the effects of RSL3, a ferroptosis activator, on cell viability, lipid peroxidation, iron content and relative protein expression of ferroptosis in synoviocytes. ICA protects the cells from death in synoviocytes induced by LPS, via the inhibition of ferroptosis by activating the Xc-/GPX4 axis, which can be exploited as a new therapeutic strategy for synovitis.

show abstract

Section: Ica Attenuates Cell Death and Increases Cell Viability In Lpsupporting

confidence: 79%

Icariin enhances cell survival in lipopolysaccharide‑induced synoviocytes by suppressing ferroptosis via the Xc‑/GPX4 axis

Luo

Zhang

2020

Exp Ther Med

View full text Add to dashboard Cite

show abstract

“…Although there are many anti-RA drugs in clinic, such as immunosuppressants, biological agents, DMARDs, steroidal drugs, and NSAIDs, most of them are associated with long-term adverse effects and costs (Li et al, 2019b;Dai et al, 2020;Zhu et al, 2020). In addition, the CFA-induced arthritis (AIA) model and the collagen-induced arthritis model are two typical preclinical experimental animal models of RA, and the former is a classic, easy-to-measure, short duration, reliable and reproducible test animal model, which is extensively used for the preclinical evaluation of anti-RA drugs since its pathological and morphological characteristics were similar to those of human RA Pan et al, 2017;Voon et al, 2017;Zhang et al, 2019;Saleem et al, 2020). The roots of P. heterophyllum have been widely used to treat RA as a vital TCM for centuries (Editorial Committee of Traditional Chinese Medicine 1999;, but their anti-RA effect and chemical profiling have not been reported so far.…”

Section: Discussionmentioning

confidence: 99%

Chemical Composition of Pterospermum heterophyllum Root and its Anti-Arthritis Effect on Adjuvant-Induced Arthritis in Rats via Modulation of Inflammatory Responses

et al. 2020

View full text Add to dashboard Cite

Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease without effective and beneficial drugs. Many traditional folk medicines have been proven to be effective in treating RA. Among these, the root of Pterospermum heterophyllum Hance has been widely used as a traditional remedy against RA in China, but there is no scientific basis yet. The aim of this study was to investigate for the first time the chemical compositions and therapeutic effect of P. heterophyllum on adjuvant-induced arthritis (AIA) model in rats. 73 compounds were identified from P. heterophyllum based on ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-qTOF-MS/MS), and flavonoids may be partly responsible for the major anti-arthritic effect. In parallel, the P. heterophyllum extract at 160, 320, and 640 mg/kg/day were orally administered to rats for 22 days after post-administration adjuvant. The results showed that P. heterophyllum remarkably ameliorated histological lesions of the knee joint, increased body weight growth, decreased arthritis score, reduced thymus and spleen indices in model rats. Moreover, P. heterophyllum treatment persuasively downregulated the levels of rheumatoid factor (RF), C-reactive protein (CRP), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), IL-6, IL-17, cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX) and matrix metalloproteinase-2 (MMP-2), and observably upregulated IL-4 and IL-10 levels in model rats. These findings suggest that P. heterophyllum has a prominent anti-RA effect on AIA rats by modulating the inflammatory responses, and supports the traditional folk use of this plant.

show abstract

“…Hs-CRP and ESR were commonly used in the laboratory to diagnose the activity of rheumatic diseases, and can be used as indicators to monitor the activity of diseases and evaluate the treatment stability. Association rules show that compared with single use Chinese traditional medicine group, XFC combined traditional Chinese medicine on IL-11, IL-17, PD-L2, hs-CRP, anti-CCP have obvious correlation, showed that after the combined traditional Chinese medicine can significantly improve the indicators, and the mechanism may be related to the pharmacological effects of XFC, semen coicis extract exerts anti-RA effects via inhibiting pro-inflammatory factors and alleviating oxidative stress [28];The active components of radix astragali can strengthened humoral and cellular immunity and improved the regulation of human immunity [29].…”

Section: Analysis Of Association Rules Between the Improvement Of Patmentioning

confidence: 99%

Screening and Verification of Key Proteins in Patients with Rheumatoid Arthritis and Intervention of Traditional Chinese Medicine

Zhang

Xin

Jiang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The aim of this study was to simultaneously and quantitatively assess the expression levels of rheumatoid arthritis (RA) proteins in serum, and explore the intervention mechanism of traditional Chinese medicine. Methods: The differences between patients and controls were investigated by Raybiotech cytokine antibody microarray. Differentially expressed proteins between two groups were then used to build receiver operator characteristic (ROC) curves. The subsequent validation with enzyme-linked immunosorbent assay (ELISA) was widened to a larger number of RA (n=40) and healthy controls (n=10).Changes of erythrocyte sedimentation rate (ESR), high-sensitivity C-reactive protein (hs-CRP), rheumatoid factors (RF) and anti-citrullinated peptide autoantibodies (anti-CCP) in patients were statistically analyzed. Results: Six proteins interleukin-2 (IL-2), interleukin-5 (IL-5), interleukin-11 (IL-11), interleukin-17 (IL-17), tumor necrosis factor-β (TNF-β), cytotoxic T lymphocyte associated antigen (CTLA)-4 were significantly up-regulated in RA compared with controls, while interleukin-8 (IL-8), programmed death-ligand 2 (PD-L2) and B7-2 were significantly down-regulated. ELISA was performed to verify the three proteins with the most significant differences, and the results consistent with the antibody chip results. The highest diagnostic accuracy using a ROC curve was observed for IL-11 with an area under the curve of 1.00. Further, after treatment, IL-11, IL-17 and PD-L2 levels regulated, and compared with the herbs group (TC), xinfeng capsule (XFC) combination with herbs (UC) more regulated. The association rule results showed that the combination of XFC with herbs significantly improved IL-11, IL-17, PD-L2 and hs-CRP. Conclusions: This study highlights the potential of antibody arrays to diagnose rheumatoid arthritis. IL-11, IL-17 and PD-L2 regulated by XFC combined with herbs.

show abstract

Coix lachryma-jobi extract ameliorates inflammation and oxidative stress in a complete Freund’s adjuvant-induced rheumatoid arthritis model

Cited by 46 publications

References 38 publications

Icariin enhances cell survival in lipopolysaccharide‑induced synoviocytes by suppressing ferroptosis via the Xc‑/GPX4 axis

Icariin enhances cell survival in lipopolysaccharide‑induced synoviocytes by suppressing ferroptosis via the Xc‑/GPX4 axis

Chemical Composition of Pterospermum heterophyllum Root and its Anti-Arthritis Effect on Adjuvant-Induced Arthritis in Rats via Modulation of Inflammatory Responses

Screening and Verification of Key Proteins in Patients with Rheumatoid Arthritis and Intervention of Traditional Chinese Medicine

Contact Info

Product

Resources

About