Collolechiarevisited and a re-assessment of ascus characteristics inPlacynthiaceae(Peltigerales, Ascomycota)

Abstract

This is the published version of a paper published in The Lichenologist. Citation for the original published paper (version of record):Kosuthova, A., Fernández-Brime, S., Westberg, M., Wedin, M. (2016) Collolechia revisited and a re-assessment of ascus characteristics in Placynthiaceae (Peltigerales, Ascomycota). Collolechia revisited and a re-assessment of ascus characteristics in Placynthiaceae (Peltigerales, Ascomycota)Alica KOŠUTHOVÁ, Samantha FERNÁNDEZ-BRIME, Martin WESTBERG and Mats WEDIN Abstract: We investigated the phylogenetic relationships in the cyanolichen family Placynthiaceae to test the current generic delimitations, where the monotypic Collolechia is currently accepted as distinct, based on differences in ascospores, ascus apex characteristics and the leprose thallus. Bayesian and maximum likelihood phylogenetic analyses of two sequence marker datasets confirmed that Collolechia caesia is nested within Placynthium, and should be called Placynthium caesium (Fr.) Jatta. We reassessed the spore and ascus characteristics and showed that Placynthium caesium falls well within the variation in Placynthium and is thus yet another example of a species that differs from close relatives by its crustose-leprose thallus structure.

Rostania revised: testing generic delimitations in Collemataceae (Peltigerales, Lecanoromycetes)

“…We extracted total DNA using the Plant DNA Mini Kit (Qiagen, Hilden, Germany) following the manufacturers’ instructions. We amplified ca 0.6 kb of the small subunit of the mitochondrial rDNA (mtSSU), ca 0.6 kb of the two protein-coding genes DNA replication licensing factor mini-chromosome maintenance complex component 7 (MCM7) and the β-tubulin gene (b-tub) using the same primer combinations and PCR settings as in previous studies (Otálora et al 2013a; Košuthová et al 2016). We assembled and edited DNA sequences using Geneious version R8 (http://www.geneious.com; Kearse et al 2012).…”

Species delimitation in the cyanolichen genus Rostania

“…PCR amplifications were performed using Illustra™ Hot Start PCR beads, according to manufacturer's instructions. PCR-reactions were performed using the same settings as in previous studies [25,29,70]. We assembled and edited DNA sequences using Geneious version R8 (http://www.geneious.com) [71].…”