Coriandrum sativum Extract Prevents Alarm Substance-Induced Fear- and Anxiety-Like Responses in Adult Zebrafish

Abstract

Anxiety disorders appear to involve distinct neurobiological mechanisms and several medications are available against this mental health problem. However, pharmacological therapeutic approaches display undesirable side effects for patients, particularly when long-term therapy is required. Some evidences have suggested that Coriandrum sativum extract (CSE) provide sedative and anxiolytic effects. We investigate if CSE could attenuate anxiety-like behaviors induced by novelty and alarm substance exposures in zebrafish. Adult zebrafish were injected with vehicle, clonazepam, or CSE (25, 50 or 100 mg/kg) and submitted to novel tank test. At the end, saline or alarm substance was added and anxiety-like responses were recorded. Twenty-four hours after, fish were submitted to the light/dark test. Novelty associated with alarm substance exposure decreased distance traveled and total time mobile in novel tank, and CSE (at 50 and 100 mg/kg) prevented these alterations similarly to clonazepam. Alarm substance reduced the time spent in white compartment ( p = 0.0193 as compared with vehicle group). Clonazepam and CSE prevented this anxiogenic effect of alarm substance. CSE presents anxiolytic effects against alarm substance-induced locomotor and anxiogenic responses similarly to clonazepam. These data corroborate with the use of this plant in traditional medicine and provides a putative new pharmacological intervention for anxiety disorders.

Alternative model organisms for toxicological fingerprinting of relevant parameters in food and nutrition

“…(Xia et al 2018) Danio rerio Neurotoxicants 1 nM to 100 mM Six of the ten tested neurotoxicants provoked developmental neurotoxicity; the triple-transgenic zebrafish line can be used to assess developmental neurotoxicity during neuronal differentiation (Koiwa et al 2019) Danio rerio Caffeine 0.001 mg/L to 186.67 mg/L Caffeine at concentrations above at concentrations !0.039 mg/L significantly altered zebrafish behavior. (Steele, Mole, and Brooks 2018) Danio rerio Coriandrum sativum extract 25, 50 or 100 mg/kg Coriandrum sativum extract presents anxiolytic effects against alarm substance-induced locomotor and anxiogenic responses (Zenki et al 2020) Danio rerio D9-tetrahydrocannabinol (THC) or cannabidiol (CBD) THC (0.08, 0.4, 2 mM) or CBD (0.02, 0.1, 0.5 mM)…”

“…Particularly, time of day, age, well size, temperature, lighting conditions and volume of the exposure solution in each well. Another study performed the novel tank test as well as the light/dark test in adult zebrafish to confirm the anxiolytic action of Coriandrum sativum extract (Zenki et al 2020).…”

Zebrafish as a Useful Tool in the Research of Natural Products With Potential Anxiolytic Effects

“…This variation may be the consequence of differences in methodology and tank characteristics; for example, the light intensity: fish spend more time exploring the white compartment when light is around 250 lux, while in lights around 500 lux, fish prefer the dark compartment (Stewart et al, 2011); for that reason, protocol characteristics must be considered in the interpretation of the findings. In the present review, some literature did not specify light intensity, but most of the quoted studies (de Carvalho et al, 2019;Duarte et al, 2019;German-Ponciano et al, 2020;Zenki et al, 2020) illuminated the light/dark chamber thanks with an environmental light bulb of 75 Watts located above the aquarium top (1.8 m), producing a constant average of 975 lux, based on Maximino et al (2010a,b).…”

“…The test lasts 7 min, erratic movements, freezing, motor activity, and the distance between fish (when tested in a shoal) are measured (Speedie and Gerlai, 2008 ). Alarms substances are currently utilized to explore the potential anxiolytic effects of plant extracts as Coriandrum sativum (Zenki et al, 2020 ).…”

“…In those studies, zebrafish are advantageous with regard to allowing direct exposure in water, which is useful for elucidating the pharmacological effects of crude plant extracts (Crawford et al, 2008 ). A great variety of plants, such as Ceratonia siliqua, Coriandrum sativum, Cymbopogon citratus , and Valeriana officinalis , among others, exert anxiolytic-like effects in zebrafish (Abidar et al, 2020 ; Mendes et al, 2020; Zenki et al, 2020 ). Zebrafish are also useful for the determination of active compounds (e.g., flavonoids, polyphenols, terpenes, lignans, and tannins) that may be involved in the therapeutic actions of various plant extracts (Abidar et al, 2020 ; Lira et al, 2020 ), mainly through the activation of γ-aminobutyric acid-A (GABA A ) receptors and interactions with serotonergic and monoaminergic systems (Benneh et al, 2017 ; Serikuly et al, 2020 ).…”