<i>cotH</i>
            Genes Are Necessary for Normal Spore Formation and Virulence in
            <i>Mucor lusitanicus</i>

Szebenyi, Csilla; Gu, Ying; Gebremariam, Teclegiorgis; Kocsubé́, Sándor; Kiss-Vetráb, Sándor; Jáger, Olivér; Patai, Roland; Spisák, Krisztina; Sinka, Rita; Binder, Ulrike; Homa, Mónika; Vágvölgyi, Csaba; Ibrahim, Ashraf S.; Nagy, Gábor; Papp, Tamás

doi:10.1128/mbio.03386-22

Cited by 14 publications

(11 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The disruption of five of them led to defects in temperature adaptation and cell wall development. More importantly, their role in virulence was confirmed in a mouse model [ 20 ] ( Table 1 ). Besides some bacteria and Mucorales , CotH proteins are not found in other fungi such as Candida and Aspergillus, making them a specific target for therapeutic and diagnostic approaches [ 21 , 57 ].…”

Section: Genes Involved In the Pathogenic Potential Of Muco...mentioning

confidence: 97%

Mucorales and Mucormycosis: Recent Insights and Future Prospects

Tahiri

Lax

Cánovas-Márquez

et al. 2023

JoF

View full text Add to dashboard Cite

The classification of Mucorales encompasses a collection of basal fungi that have traditionally demonstrated an aversion to modern genetic manipulation techniques. This aversion led to a scarcity of knowledge regarding their biology compared to other fungal groups. However, the emergence of mucormycosis, a fungal disease caused by Mucorales, has attracted the attention of the clinical field, mainly because available therapies are ineffective for decreasing the fatal outcome associated with the disease. This revitalized curiosity about Mucorales and mucormycosis, also encouraged by the recent COVID-19 pandemic, has spurred a significant and productive effort to uncover their mysteries in recent years. Here, we elaborate on the most remarkable breakthroughs related to the recently discovered genetic advances in Mucorales and mucormycosis. The utilization of a few genetic study models has enabled the identification of virulence factors in Mucorales that were previously described in other pathogens. More notably, recent investigations have identified novel genes and mechanisms controlling the pathogenic potential of Mucorales and their interactions with the host, providing fresh avenues to devise new strategies against mucormycosis. Finally, new study models are allowing virulence studies that were previously hampered in Mucorales, predicting a prolific future for the field.

show abstract

Section: Genes Involved In the Pathogenic Potential Of Muco...mentioning

confidence: 97%

Mucorales and Mucormycosis: Recent Insights and Future Prospects

Tahiri

Lax

Cánovas-Márquez

et al. 2023

JoF

View full text Add to dashboard Cite

show abstract

“…The CotH family of kinases, originally found in Bacillus subtilis (involved in the production of endospores), serves as receptors for human GRP78 during mucormycosis. Szebenyi et al identified 17 homologous genes of CotH in the Mucor lusitanicus genome, and disruption of various CotH genes revealing their significant roles in pathogenesis as listed in Table …”

Section: Virulence Factors Involved In Pathogenesismentioning

confidence: 99%

“…Szebenyi et al identified 17 homologous genes of CotH in the Mucor lusitanicus genome, and disruption of various CotH genes revealing their significant roles in pathogenesis as listed in Table 2. 98 The pathogenicity of mucormycosis etiological agents relies on the number of CotH genes as observed in the genome of Rhizopus, Mucor, and Lichtheimia genera, which are associated with invasive infections. 88,100,101 Structural analysis revealed analogous interactions between GRP78 and CotH3 and CotH2 via modeling and docking.…”

Section: Pathogenesismentioning

confidence: 99%

Comprehensive Review on the Virulence Factors and Therapeutic Strategies with the Aid of Artificial Intelligence against Mucormycosis

Tanwar,

Singh,

Singh

et al. 2024

ACS Infect. Dis.

View full text Add to dashboard Cite

Mucormycosis, a rare but deadly fungal infection, was an epidemic during the COVID-19 pandemic. The rise in cases (COVID-19-associated mucormycosis, CAM) is attributed to excessive steroid and antibiotic use, poor hospital hygiene, and crowded settings. Major contributing factors include diabetes and weakened immune systems. The main manifesting forms of CAM�cutaneous, pulmonary, and the deadliest, rhinocerebral� and disseminated infections elevated mortality rates to 85%. Recent focus lies on small-molecule inhibitors due to their advantages over standard treatments like surgery and liposomal amphotericin B (which carry several long-term adverse effects), offering potential central nervous system penetration, diverse targets, and simpler dosing owing to their small size, rendering the ability to traverse the blood−brain barrier via passive diffusion facilitated by the phospholipid membrane. Adaptation and versatility in mucormycosis are facilitated by a multitude of virulence factors, enabling the pathogen to dynamically respond to various environmental stressors. A comprehensive understanding of these virulence mechanisms is imperative for devising effective therapeutic interventions against this highly opportunistic pathogen that thrives in immunocompromised individuals through its angio-invasive nature. Hence, this Review delineates the principal virulence factors of mucormycosis, the mechanisms it employs to persist in challenging host environments, and the current progress in developing small-molecule inhibitors against them.

show abstract

“…They facilitate quantification of messenger RNA (mRNA) or transfer RNA (tRNA) through quantitative PCR, reverse transcriptase PCR and modified-induced misincorporation tRNA sequencing (mim-tRNASeq) ( 78 , 79 ). The gene editing tool CRISPR/Cas9 utilises guide RNA which matches with target gene (CRISPR) and CRISPR-associated protein 9 (Cas9), an endonuclease which helps to break the dsDNA and facilitate editing of the target gene ( 80 ) and it can be used for loss-of-function studies ( 12 , 45 , 81 ). Bioinformatics tools have been developed and adapted for genomic studies across microbial, Drosophila and human genomes and these allow for rapid screening of genomes and vast publicly available pathogen and fly data for potential targets for further study ( 79 , 82 ).…”

Section: Application Of Drosophila To Human Fungal...mentioning

confidence: 99%

The utility of Drosophila melanogaster as a fungal infection model

Mpamhanga,

Kounatidis

2024

Front. Immunol.

View full text Add to dashboard Cite

Invasive fungal diseases have profound effects upon human health and are on increase globally. The World Health Organization (WHO) in 2022 published the fungal priority list calling for improved public health interventions and advance research. Drosophila melanogaster presents an excellent model system to dissect host-pathogen interactions and has been proved valuable to study immunopathogenesis of fungal diseases. In this review we highlight the recent advances in fungal-Drosophila interplay with an emphasis on the recently published WHO’s fungal priority list and we focus on available tools and technologies.

show abstract

cotH Genes Are Necessary for Normal Spore Formation and Virulence in Mucor lusitanicus

Abstract: Current treatment options for mucormycosis are inadequate, resulting in high mortality rates, especially among immunosuppressed patients. The development of novel therapies for mucormycosis has been hampered by lack of understanding of the pathogenetic mechanisms.

Cited by 14 publications

References 65 publications

Mucorales and Mucormycosis: Recent Insights and Future Prospects

Mucorales and Mucormycosis: Recent Insights and Future Prospects

Comprehensive Review on the Virulence Factors and Therapeutic Strategies with the Aid of Artificial Intelligence against Mucormycosis

The utility of Drosophila melanogaster as a fungal infection model

Contact Info

Product

Resources

About