<i>Cycle</i> affects imidacloprid efficiency by mediating cytochrome P450 expression in the brown planthopper <i>Nilaparvata lugens</i>

Kang, Kui Dong; Pan, Yang; Pang, Rui; Yue, Lei; Zhang, W.

doi:10.1111/imb.12313

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…Each treatment was performed in triplicate. qRT-PCR also used a previously described method [49]. The primers used for dsRNA preparation and real-time PCR are listed in Table S5.…”

Section: Methodsmentioning

confidence: 99%

Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens

et al. 2018

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BackgroundThe insect gustatory system plays a central role in the regulation of multiple physiological behaviors and the co-evolution between insects and their hosts. The gustatory receptors (Gr) are important to allow insects to sense their environment. It is critical to the selection of foods, mates and oviposition sites of insects. In this study, the Gr family genes of the brown planthopper (BPH) Nilaparvata lugens Stål (Hemiptera: Delphacidae) were identified and analyzed, and their potential relationship to the fecundity of BPH was explored by RNA interference (RNAi).ResultsWe identified 32 putative Gr genes by analyzing transcriptome and genome data from BPH. Most of these Gr proteins have the typical structure of seven transmembrane domains. The BPH Gr genes (NlGrs) were expressed in virtually all tissues and stages, whilst higher transcript accumulations were found in adult stages and in the midguts of females. Based on the phylogenic analysis, we classified NlGrs into five potential categories, including 2 sugar receptors, 2 Gr43a-like receptors, 7 CO2 receptors, 5 bitter receptors and 13 NlGrs with unknown functions. Moreover, we found that 10 NlGrs have at least two alternative splicing variants, and obtained alternative splicing isoforms of 5 NlGrs. Finally, RNAi of 29 NlGrs showed that 27 of them are related to the transcript levels of two fecundity related genes vitellogenin and vitellogenin receptor.ConclusionsWe found 32 Gr genes in BPH, among which at least 27 are required for normal expression of fecundity markers of this insect pest. These findings provide the basis for the functional study of Grs and the exploration of potential genes involved in the monophagous character of BPH.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5391-5) contains supplementary material, which is available to authorized users.

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“…Each treatment was performed in triplicate. qRT-PCR also used a previously described method [49]. The primers used for dsRNA preparation and real-time PCR are listed in Table S5.…”

Section: Methodsmentioning

confidence: 99%

Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens

et al. 2018

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“…The study by Kang et al provides the evidence that the circadian cycles of Nilaparvata lugens affect the effectiveness of imidacloprid. The scientists explain this by two peaks in the expression of cytochrome P450 genes during the day [Kang et al 2017]. When studying the effect of deltamethrin on Anopheles gambiae mosquitoes, it was shown that among the metabolic detoxification enzymes there are those that depend on the time of day (glutathione-S-transferases) and have constant activity (oxidases and esterases) [Balmert et al 2014].…”

Section: Annual and Circadian Rhythms Of Organism Activitymentioning

confidence: 99%

Intraspecific Sensitivity to Toxicants – a Methodological Problem of Bioassay: Review

Olkova¹

2021

J. Ecol. Eng.

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The article discusses the scientific studies that reveal the mechanisms of intraspecific differences in responses of organisms to chemical exposure. The factors of individual sensitivity to toxicants are represented by genetic differences between individuals of the same species and genetically unconditioned factors. The genetic factors are mutations, including under-researched mitochondrial DNA mutations and genomic drift, genetic polymorphism, and gender-related differences. Age, conditions of keeping and state of organisms, seasonal variations of body functions, and biotic interactions are considered as the factors that are not related to the genetic apparatus. The article considers a number of works, in which the effects of the combined action of external factors on sensitivity of organisms in model groups are studied.

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Chrononutrition – ‘The Clock Makes Good Food’

Steinberg

2018

Aquatic Animal Nutrition

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Cycle affects imidacloprid efficiency by mediating cytochrome P450 expression in the brown planthopper Nilaparvata lugens

Cited by 6 publications

References 28 publications

Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens

Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens

Intraspecific Sensitivity to Toxicants – a Methodological Problem of Bioassay: Review

Chrononutrition – ‘The Clock Makes Good Food’

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