Davallia bilabiata Inhibits TNF-α-Induced Adhesion Molecules and Chemokines by Suppressing IKK/NF-kappa B Pathway in Vascular Endothelial Cells

Abstract

Davallia bilabiata (D. bilabiata) is also called GuSuiBu in Taiwan and is used as a substitute for Drynaria fortunei J. Sm. It is often used for trauma and bone repair. The inhibitory effect of D. bilabiata on inflammatory activity has not been reported. In the present study, we aimed to study the mechanism of anti-inflammation of D. bilabiata on the adhesion of leukocytes to vascular endothelial cells. The results showed that D. bilabiata, at concentrations without cytotoxic effect, inhibited the adhesion of monocytes (THP-1) to the TNF-α-stimulated human umbilical vascular endothelial cells (HUVECs). D. bilabiata suppressed the expression of the adhesion molecules ICAM, VCAM, and E-selectin at both the mRNA and protein level. In addition, both of the TNF-α-induced mRNA and protein expression of chemokines including fractalkine/CX3CL1, MCP-1 and RANTES as well as the level of secreted soluble fractalkine were decreased by D. bilabiata. We also verified that D. bilabiata inhibited the TNF-α-induced nuclear translocation of NF-κB through the inhibitory process on the TNF-α-activated phosphorylation of IKKα, IKKβ, IκB and NF-κB. All together, we concluded that the D. bilabiata affected the canonical pathway of TNF-α-induced NF-κB activation and down-regulated cell adhesion molecules and chemokine expression through inhibition of the NF-κB/IκBα/IKK signaling pathway. These findings strongly indicated that D. bilabiata might be a promising alternative/adjunct treatment for inflammatory diseases, such as rheumatoid arthritis and osteoarthritis.

Aspirin modulates the inflammatory response in a thrombus‑stimulated LMVEC model

“…The regulatory mechanisms associated with CX3CL1 may also involve NF-κB. Yang et al (23) suggested that TNF-α promotes the adhesion of monocyte THP-1 to human umbilical vein endothelial cells via the regulation of adhesion molecules by the NF-κB signaling pathway, including the expression of chemokine CX3CL1/monocyte chemotactic protein-1 of ICAM/vascular cell adhesion molecule/S-select in. Cimato et al (24) found that inflammatory cytokines, rather than cholesterol, regulated the expression of CX3CL1 in atherosclerosis.…”

Antimicrobial activity of the chloroform fraction of <i>Drynaria <i>fortunei</i></i> against oral pathogens

“…The CHCl 3 extract contains naringin, hesperidin, caffeic acid, and procyanidin, but flavonoids was detected only in the n-BuOH extract for presence of organic acid (21,22,25,28,43). Some of the polyphenols isolated from plants exhibit anti-carious effects such as growth inhibition and inhibition of glucosyltransferases against mutans streptococci (44)(45)(46).…”

“…Drynaria fortunei (D. fortunei; Gol-Se-Bo in Korean and Gu-Sui-Bu in Chinese), a traditional Korean medicine, has been known to be effective in treating inflammation, hyperlipidemia, arteriosclerosis, rheumatism, gynecological diseases, osteoporosis, and bone resorption (21)(22)(23)(24)(25). It is also used for treatment and prevention of orthopedic diseases, and is known to promote bone healing (22,26).…”

THE CLINICAL AND PATHOGENETIC SIGNIFICANCE OF 70- AND 27-kDa HEAT SHOCK PROTEINS IN OSTEOARTHRITIS

“…Хондроциты и остеобласты, подвергшиеся цитокиновой атаке, не способны по тем или иным причинам полноценно от-ветить усиленной экспрессией БТШ70 и БТШ27. Разви-тие сценария внутриклеточной сигнализации через акти-вацию фактора транскрипции NF-κB, как известно, ве-дет к экспрессии матриксных металлопротеиназ, цито-кинов, приводя к деградации межклеточного матрикса [18]. Мы предполагаем, что в дальнейшем процесс пато-генеза ОА переходит в фазу местной тканевой реакции, где существенную роль играют процессы гипертрофиче-ской дифференцировки хондроцитов и остеоподобных клеток субхондральной кости [19].…”