<i>De novo</i>designed coiled coils as scaffolds for lanthanides, including novel imaging agents with a twist

Webster, Alexandra M.; Peacock, Anna F. A.

doi:10.1039/d1cc02013g

Cited by 15 publications

(12 citation statements)

References 128 publications

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“…These high affinity de novo designed proteins may offer a new class of scaffolds for lanthanides, including imaging agents, and the design of functional lanthanide‐protein assemblies. [ 16,17 ]…”

Section: Introductionmentioning

confidence: 99%

Repeated Recovery of Rare Earth Elements Using a Highly Selective and Thermo‐Responsive Genetically Encoded Polypeptide

Hussain

Kim

Cho

et al. 2021

Adv Funct Materials

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Rare earth elements (REEs) have become increasingly important materials owing to their use in the high-tech and clean-energy industries. However, the unpredictable supply, possible health risks, and environmentally unsustainable extraction practices associated with REEs have encouraged the development of green technologies for the selective extraction and recovery of metals. This study presents a simple and innovative approach for the selective extraction and recovery of total REEs. Elastin-like polypeptide (ELP) and the REE-binding domain (lanmodulin) are fused to form REEs-sensitive and thermo-responsive genetically encoded ELP called RELP, where ELP offered a reversible, inverse phase transition for repeated uses. The RELP are purified and used for the selective extraction of total REEs from competing non-REEs metals by controlling the solution temperature (4 and 37 °C) and pH. RELP exhibit high REE specificity, even in the presence of non-REE metal ions. The bound REEs are readily recovered during at least six repeated cycles, and the efficiency is maintained. Moreover, REEs are selectively recovered by RELP from steel slag leachate, a potential industrial source of REEs. RELP offers a rapid, selective, and scalable method for REE extraction and recovery. This technology can be adapted to recover other precious metals and commodities.

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Section: Introductionmentioning

confidence: 99%

Repeated Recovery of Rare Earth Elements Using a Highly Selective and Thermo‐Responsive Genetically Encoded Polypeptide

Hussain

Kim

Cho

et al. 2021

Adv Funct Materials

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“…55 Peptide-PAINT is a lucrative potential alternative for DNA-PAINT as the rational design of peptides is gaining traction of late. 56,57…”

Section: Genetically Modified Tags As Labelling Probesmentioning

confidence: 99%

Labeling approaches for DNA-PAINT super-resolution imaging

2023

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“…This area has been pioneered by DeGrado, 7 Angela Lombardi, 157 Pecoraro and others, and is being advanced by them and others such as Anderson, 17 Brian Kuhlman and Hilvert, 15,96 Anna Peacock. 158 Revolution in protein design: The emergence and establishment of computational approaches…”

Section: Coiled-coil Assemblies As a Special And Particularly Accessi...mentioning

confidence: 99%

A Brief History of De Novo Protein Design: Minimal, Rational, and Computational

Woolfson

2021

Journal of Molecular Biology

116

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De novodesigned coiled coils as scaffolds for lanthanides, including novel imaging agents with a twist

Abstract: For much of their history, lanthanides were thought to be biologically inert. However, the last decade has seen the discovery and development of the field of native lanthanide biochemistry. Lanthanides...

Cited by 15 publications

References 128 publications

Repeated Recovery of Rare Earth Elements Using a Highly Selective and Thermo‐Responsive Genetically Encoded Polypeptide

Repeated Recovery of Rare Earth Elements Using a Highly Selective and Thermo‐Responsive Genetically Encoded Polypeptide

Labeling approaches for DNA-PAINT super-resolution imaging

A Brief History of De Novo Protein Design: Minimal, Rational, and Computational

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