<i>De novo</i> genome assembly of the tobacco hornworm moth (<i>Manduca sexta)</i>

Gershman, Ariel; Romer, Tatiana Gelaf; Fan, Yunfan; Razaghi, Roham; Smith, Wendy A.; Timp, Winston

doi:10.1093/g3journal/jkaa047

Cited by 26 publications

(37 citation statements)

References 65 publications

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“…Except for a few cases with duplicate receptors for which the probes could cross-target, our probes did not have off-targets. The recent de novo M. sexta genome assembly (Gershman et al, 2021) indicated that MsexOR84 and MsexOR89 might be the same gene, and similarly MsexOR42 and MsexOR66 might be the same gene. Therefore, we provisionally named these receptors MsexOR84/89 and MsexOR42/66.…”

Section: Nanostring Gene Expression Assaymentioning

confidence: 99%

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

Tom

Llorca²,

Bucks³

et al. 2022

Front. Ecol. Evol.

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For the nocturnal hawkmoth Manduca sexta, olfactory and gustatory cues are essential for finding partners, food, and oviposition sites. Three chemosensory receptor families, odorant receptors (ORs), ionotropic receptors (IRs), and gustatory receptors (GRs) are involved in the detection of these stimuli. While many chemosensory receptor genes have been identified, knowledge of their expression profile in potentially chemoreceptive organs is incomplete. Here, we studied the expression patterns of chemosensory receptors in different tissues including the antennae, labial palps, proboscis, legs, wings and ovipositor. We compared the receptors’ expression in female and male moths both before and after mating by using the NanoString platform. This tool allowed us to measure expression levels of chemosensory receptor genes in a single reaction using probes designed against 71 OR, 29 IR and 49 GR transcripts. In all tissues investigated, we detected expression of genes from all three receptor families. The highest number of receptors was detected in the antennae (92), followed by the ovipositor (59), while the least number was detected in the hindlegs (21). The highest number of OR genes were expressed in the antennae (63), of which 24 were specific to this main olfactory organ. The highest number of IRs were also expressed in the antennae (16), followed by the ovipositor (15). Likewise, antennae and ovipositor expressed the highest number of GRs (13 and 14). Expression of the OR co-receptor MsexORCo, presumably a prerequisite for OR function, was found in the antennae, labial palps, forelegs and ovipositor. IR co-receptors MsexIR25a and MsexIR76b were expressed across all tested tissues, while expression of the IR co-receptor MsexIR8a was restricted to antennae and ovipositor. Comparing the levels of all 149 transcripts across the nine tested tissues allowed us to identify sex-biased gene expression in the antennae and the legs, two appendages that are also morphologically different between the sexes. However, none of the chemosensory receptors was differentially expressed based on the moths’ mating state. The observed gene expression patterns form a strong base for the functional characterization of chemosensory receptors and the understanding of olfaction and gustation at the molecular level in M. sexta.

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Section: Nanostring Gene Expression Assaymentioning

confidence: 99%

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

Tom

Llorca²,

Bucks³

et al. 2022

Front. Ecol. Evol.

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“…Evidence of a single segregating inversion on chromosome 12 from the newly released M. sexta assembly (Gershman et al . 2021).…”

Section: Resultsmentioning

confidence: 99%

“…fragmented) genome assembly for several years (Kanost et al . 2016); much more recently a chromosome-level assembly has been published (Gershman et al . 2021).…”

Section: Methodsmentioning

confidence: 99%

Population differentiation and structural variation in theManduca sextagenome across the United States

Mongue

Kawahara

2020

Preprint

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Many species that are extensively studied in the laboratory are less well characterized in their natural habitat, and laboratory strains represent only a small fraction of the variation in the genome of the species. Here we investigate genomic variation in three natural populations of an agricultural pest and a model insect for many scientific disciplines, the tobacco hornworm (Manduca sexta). We show that hornworms from Arizona, Kansas, and North Carolina are genetically distinct, with Arizona being particularly differentiated from the other two populations. Specifically, two segregating inversions and a potential pseudogene are found only in the Arizona population. One inversion on the Z chromosome may enhance adaptive evolution of the sex chromosome, while the significance of the other, autosomal inversion remains unclear. The pseudogene may be involved in the exploitation of a novel hostplant in Arizona, but functional genetic assays will be required to confirm this hypothesis. Nevertheless, our results reveal undiscovered natural variation and provide useful genomic data for a model insect species.

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“…Genome mining via tBLASTn-and BLASTp-searches identified a close paralog to Hi-1, XM_030179956, hereinafter referred to as Hi-like. Hi-like and Hi-1 were linked and exhibited 85% coding sequence identity (Fig S6) (Gershman et al 2021). However, recombinant protein of Hi-like (Fig.…”

Section: Targeted Mutagenesis Of Hi-1 By Crispr/cas9 and Off-target A...mentioning

confidence: 99%

A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant

Lin

Silven

Wybouw

et al. 2022

Preprint

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Green leaf volatiles (GLVs) are short-chain oxylipins that are emitted from plants in response to stress. Previous studies have shown that oral secretions (OS) of the tobacco hornworm Manduca sexta, introduced into plant wounds during feeding, catalyze the re-arrangement of GLVs from Z-3- to E-2-isomers. This change in the volatile signal however is bittersweet for the insect as it can be used by their natural enemies, as prey location cue. Here we identified a (3Z):(2E)-hexenal isomerase (Hi-1) in M. sexta’s OS that catalyzes the conversion of the GLV Z-3-hexenal to E-2-hexenal. Hi-1 mutants that were raised on a GLV-free diet showed developmental disorders, indicating that Hi-1 also metabolizes other substrates important for the insect’s development. Phylogenetic analysis placed Hi-1 within the GMCβ-subfamily and showed that Hi-1 homologs from other lepidopterans could catalyze similar reactions. Our results indicate that Hi-1 not only modulates the plant’s GLV-bouquet but also functions in insect development.

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De novo genome assembly of the tobacco hornworm moth (Manduca sexta)

Cited by 26 publications

References 65 publications

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

Population differentiation and structural variation in theManduca sextagenome across the United States

A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant

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