<i>Diaphorina citri</i>populations carrying the bacterial plant pathogen<i>Candidatus</i>Liberibacter asiaticus are more susceptible to infection by entomopathogenic fungi than bacteria-free populations

Orduño-Cruz, Nuvia; Guzmán‐Franco, Ariel W.; Rodríguez‐Leyva, Esteban

doi:10.1111/afe.12138

Cited by 8 publications

(5 citation statements)

References 22 publications

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“…Confocal microscopy reveals extensive nuclear morphological changes in the guts of adult insects that have acquired the pathogen, , although D. citri nymphs are resistant to these effects , D. citri exposed to C Las are also more susceptible to an entomopathanogenic fungus as well as certain insecticides. , These findings demonstrate that C Las elicits an immune response from D. citri and underscores the importance of understanding the insect immune system’s role in vector–Liberibacter interactions.…”

Section: Introductionmentioning

confidence: 73%

Candidatus Liberibacter asiaticus Minimally Alters Expression of Immunity and Metabolism Proteins in Hemolymph of Diaphorina citri, the Insect Vector of Huanglongbing

et al. 2018

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Huanglongbing (HLB), also known as citrus greening disease, is the most serious disease of citrus plants. It is associated with the Gram-negative bacterium ' Candidatus Liberibacter asiaticus' ( CLas), which is transmitted between host plants by the hemipteran insect vector Diaphorina citri in a circulative, propagative manner involving specific interactions with various insect tissues including the hemolymph, fluid that occupies the body cavity akin to insect blood. High resolution quantitative mass spectrometry was performed to investigate the effect of CLas exposure on D. citri hemolymph at the proteome level. In contrast to the broad proteome effects on hundreds of proteins and a diverse array of metabolic pathways previously reported in gut and whole insect proteome analyses, the effect of CLas on the hemolymph was observed to be highly specific, restricted to key immunity and metabolism pathways, and lower in magnitude than that previously observed in the whole insect body and gut. Vitellogenins were abundantly expressed and CLas-responsive. Gene-specific RNA expression analysis suggests that these proteins are expressed in both male and female insects and may have roles outside of reproductive vitellogenesis. Proteins for fatty acid synthesis were found to be up-regulated, along with metabolic proteins associated with energy production, supported at the organismal level by the previously published observation that D. citri individuals experience a higher level of hunger when reared on CLas-infected plants. Prediction of post-translational modifications identified hemolymph proteins with phosphorylation and acetylation upon CLas exposure. Proteins derived from the three most prominent bacterial endosymbionts of the psyllid were also detected in the hemolymph, and several of these have predicted secretion signals. A DNAK protein, the bacterial HSP70, detected in the hemolymph expressed from Wolbachia pipientis was predicted to encode a eukaryotic nuclear localization signal. Taken together, these data show specific changes to immunity and metabolism in D. citri hemolymph involving host and endosymbiont proteins. These data provide a novel context for proteomic changes seen in other D. citri tissues in response to CLas and align with organismal data on the effects of CLas on D. citri metabolism and reproduction.

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Section: Introductionmentioning

confidence: 73%

Candidatus Liberibacter asiaticus Minimally Alters Expression of Immunity and Metabolism Proteins in Hemolymph of Diaphorina citri, the Insect Vector of Huanglongbing

et al. 2018

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“…citri suggest CLas has significant and systemic effects on D . citri defense, metabolism, cellular stress, and microbiota [ 22 – 34 , 19 ], and provide potential responses that may be important in restricting CLas propagation or promoting CLas survival. However, some of the impacts of CLas on D .…”

Section: Introductionmentioning

confidence: 99%

Development on Citrus medica infected with ‘Candidatus Liberibacter asiaticus’ has sex-specific and -nonspecific impacts on adult Diaphorina citri and its endosymbionts

et al. 2020

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Huanglongbing (HLB) is a deadly, incurable citrus disease putatively caused by the unculturable bacterium, 'Candidatus Liberibacter asiaticus' (CLas), and transmitted by Diaphorina citri. Prior studies suggest D. citri transmits CLas in a circulative and propagative manner; however, the precise interactions necessary for CLas transmission remain unknown, and the impact of insect sex on D. citri-CLas interactions is poorly understood despite reports of sex-dependent susceptibilities to CLas. We analyzed the transcriptome, proteome, metabolome, and microbiome of male and female adult D. citri reared on healthy or CLas-infected Citrus medica to determine shared and sex-specific responses of D. citri and its endosymbionts to CLas exposure. More sex-specific than shared D. citri responses to CLas were observed, despite there being no difference between males and females in CLas density or relative abundance. CLas exposure altered the abundance of proteins involved in immunity and cellular and oxidative stress in a sex-dependent manner. CLas exposure impacted cuticular proteins and enzymes involved in chitin degradation, as well as energy metabolism and abundance of the endosymbiont 'Candidatus Profftella armatura' in both sexes similarly. Notably, diaphorin, a toxic Profftella-derived metabolite, was more abundant in both sexes with CLas exposure. The responses reported here resulted from a combination of CLas colonization of D. citri as well as the effect of CLas infection on C. medica. Elucidating these impacts on D. citri and their endosymbionts contributes to our understanding of the HLB pathosystem and identifies the responses potentially critical to limiting or promoting CLas acquisition and propagation in both sexes.

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“…An important factor to be considered is the interaction between D. citri infection by entomopathogenic fungi and the presence of Candidatus liberibacter in the insect's body. Orduño-Cruz et al (2015) demonstrated, under laboratory conditions, that individuals of D. citri carrying Candidatus liberibacter asiaticus were more susceptible to fungal infection by isolates of B. bassiana, C. fumosorosea, and Metarhizium anisopliae than CLas-free individuals. This result was consistent for the three isolates tested despite having different virulence levels against D. citri adults.…”

Section: Resultsmentioning

confidence: 96%

Use and perspective of entomopathogenic fungi biocontrol agents of Asian citrus psyllid (Diaphorina citri): A bibliometric review

Portes,

Pimentel,

Zawadneak

2023

RSD

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The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is the main insect vector of the bacteria Candidatus liberibacter spp., which are the causative agents of Citrus Greening disease, also known as Huanglongbing (HLB). The current lack of treatment for the disease means that intensive work is required to manage psyllid populations, mainly through chemical control methods. Since biological control has become a promising management strategy and entomopathogenic fungi are one of the components of integrated pest management, this review aimed to compare previously published results regarding the most efficient fungi for controlling D. citri and to gather information about the best options available. To this end, the bibliography indexed in the Scopus and Web of Science databases was analyzed using bibliometric tools with the R Studio software and the Bibliometrix package. In recent years, Cordyceps fumosorosea, Beauveria bassiana, and Hirsutella citriformis were identified as the most relevant fungus species, showing efficient results. In addition, the countries that publish the most on this subject are also among the largest citrus producers. Studies performed under greenhouse and field conditions are still scarce but necessary to validate the results obtained in the laboratory, as well as those that explore the mechanisms of action involved in the pathogenicity of fungi against D. citri, which are matters to consider in future assessments.

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Diaphorina citripopulations carrying the bacterial plant pathogenCandidatusLiberibacter asiaticus are more susceptible to infection by entomopathogenic fungi than bacteria-free populations

Cited by 8 publications

References 22 publications

Candidatus Liberibacter asiaticus Minimally Alters Expression of Immunity and Metabolism Proteins in Hemolymph of Diaphorina citri, the Insect Vector of Huanglongbing

Candidatus Liberibacter asiaticus Minimally Alters Expression of Immunity and Metabolism Proteins in Hemolymph of Diaphorina citri, the Insect Vector of Huanglongbing

Development on Citrus medica infected with ‘Candidatus Liberibacter asiaticus’ has sex-specific and -nonspecific impacts on adult Diaphorina citri and its endosymbionts

Use and perspective of entomopathogenic fungi biocontrol agents of Asian citrus psyllid (Diaphorina citri): A bibliometric review

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