<i>Diaporthe limonicola</i> Causing Leaf Spot Disease on <i>Areca catechu</i> in China

Xu, Gang; Qiu, F.; Li, X.; Zheng, F. Q.; Zheng, Linling; Miao, Weiguo; Xie, Changping

doi:10.1094/pdis-11-19-2324-pdn

Cited by 5 publications

(1 citation statement)

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“…A preliminary identification, based on BLASTn searches with the ITS sequences of the isolates from the present study, was carried out to determine the most closely related taxa, whose sequences were subsequently retrieved from GenBank. Species of Diaporthe isolated from palm tissues listed in the recent literature [26,66,67] or deposited in GenBank were also used. A total of 127 strains currently accepted in the genus Diaporthe were used to perform an initial phylogenetic analysis based on the ITS sequences.…”

Section: Sequence Alignment and Phylogenetic Analysesmentioning

confidence: 99%

Diaporthe Species on Palms: Molecular Re-Assessment and Species Boundaries Delimitation in the D. arecae Species Complex

Pereira,

Hilário,

Gonçalves

et al. 2023

Microorganisms

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Due to cryptic diversification, phenotypic plasticity and host associations, multilocus phylogenetic analyses have become the most important tool in accurately identifying and circumscribing species in the Diaporthe genus. However, the application of the genealogical concordance criterion has often been overlooked, ultimately leading to an exponential increase in novel Diaporthe spp. Due to the large number of species, many lineages remain poorly understood under the so-called species complexes. For this reason, a robust delimitation of the species boundaries in Diaporthe is still an ongoing challenge. Therefore, the present study aimed to resolve the species boundaries of the Diaporthe arecae species complex (DASC) by implementing an integrative taxonomic approach. The Genealogical Phylogenetic Species Recognition (GCPSR) principle revealed incongruences between the individual gene genealogies. Moreover, the Poisson Tree Processes’ (PTPs) coalescent-based species delimitation models identified three well-delimited subclades represented by the species D. arecae, D. chiangmaiensis and D. smilacicola. These results evidence that all species previously described in the D. arecae subclade are conspecific, which is coherent with the morphological indistinctiveness observed and the absence of reproductive isolation and barriers to gene flow. Thus, 52 Diaporthe spp. are reduced to synonymy under D. arecae. Recent population expansion and the possibility of incomplete lineage sorting suggested that the D. arecae subclade may be considered as ongoing evolving lineages under active divergence and speciation. Hence, the genetic diversity and intraspecific variability of D. arecae in the context of current global climate change and the role of D. arecae as a pathogen on palm trees and other hosts are also discussed. This study illustrates that species in Diaporthe are highly overestimated, and highlights the relevance of applying an integrative taxonomic approach to accurately circumscribe the species boundaries in the genus Diaporthe.

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Section: Sequence Alignment and Phylogenetic Analysesmentioning

confidence: 99%