<i>Dicrocoelium</i> ova can block the induction phase of experimental autoimmune encephalomyelitis

Navi, Zahra; Fata, Abdolmajid; Rad, Mozhdeh Jafari; Mogaddas, Elham; Mahmoudi, Mahmoud; Rastin, Maryam; Bazaz, Mojtaba Mousavi; Arab, Fahimeh Lavi; Negah, Sajad Sahab; Heidari, Amir Reza

doi:10.1111/pim.12792

Cited by 5 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So far, only one study has been conducted on the protective impact of this parasite. Navi et al have reported that D.dendriticum infection with elevated IL-4 cytokines and reduced IFN-Y cytokine responses resulted in a reduction of clinical and pathological signs, as well as a delay in disease progression in the EAE model (31). Our results also emphasize the role of IL-4 in reducing in ammatory and pathological responses in DSS-induced colitis mice.…”

Section: Discussionsupporting

confidence: 78%

“…Helminths or parasitic worms as the immune regulators can with skew the immune system towards Th2 and Treg responses and inhibit pro-in ammatory Th1, Th17 immune responses, and thereby reducing in ammation (12). IL4 (Th2) actively suppress Th1 pro in ammatory cells and reduce the production of TNFα and IFN-Y (13). A growing body of investigation in murine models has supports the protective roles of helminth infection and their products in modulate immune response and block ongoing in ammation (8).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Could Dicrocoelium dendriticum eggs play a potential immunotherapeutic role experimental colitis?

Mighani,

Eilakinezhad,

Esmaeili

et al. 2023

Preprint

View full text Add to dashboard Cite

Inflammatory bowel disease (IBD) significantly diminishes an individual’s quality of life and elevates the likelihood of colorectal cancer. Recent clinical and experimental findings suggest that infection with parasitic helminths can exert a suppressive influence on the development of certain inflammatory conditions. The objective of this study was to evaluate the immunoregulatory impact of Dicrocoelium eggs on experimentally induced colitis using dextran sulfate sodium (DSS) in C57BL/6 mice. C57BL/6 mice were given 3.5% DSS orally daily for 7 days to induce colitis. During this period, the mice were treated with the Dicrosoeliumeggs intraperitoneally. Colitis intensity was investigated by assessing parameters such as body weight, stool consistency or bleeding, disease activity index (DAI), colon lengths, macroscopic scores, histopathological findings, colon gene expression levels, and serum cytokine levels. Our results indicated that the administration of Dicrocoelium eggs significantly led to reduction of severity of colitis and disease index. The histopathological scores improved, which were associated with the down-regulation of IFN-γ and up-regulation of IL-4 expression. Our results imply the therapeutic potential of Dicrosoelium eggs in treating colitis. Immunotherapy involving Dicrosoelium eggs primarily induces a Th2 response and modulates IFN-Y, contributing to the reduction of inflammation in colitis. Therefore, it can be considered a promising therapeutic approach to alleviate inflammation in inflammatory bowel disease (IBD).

show abstract

Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Could Dicrocoelium dendriticum eggs play a potential immunotherapeutic role experimental colitis?

Mighani,

Eilakinezhad,

Esmaeili

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Then antibiotics were removed with three sterile PBS washes. Finally, the eggs were counted with Neobar Lam and frozen at −80°C until use 23 …”

Section: Methodsmentioning

confidence: 99%

Evaluation of the immunoregulatory effect of Dicrocoelium dendriticum eggs on inflammatory and anti‐inflammatory cytokines in EAE model

Rad

Navi

Heidari

et al. 2022

Parasite Immunology

Self Cite

View full text Add to dashboard Cite

Experimental autoimmune encephalomyelitis (EAE) is an appropriate model for the study of the immunologic and pathologic mechanisms in multiple sclerosis (MS). According to the hygiene hypothesis, helminths can improve immunoregulation and have therapeutic effects on immune‐mediated diseases. In this study, we used Dicrocoelium dendriticum (Dicrocoeliidae, Platyhelminthes) eggs for the evaluation of their prophylactic and treatment effects on EAE disease. D. dendriticum eggs were extracted. Female C57BL/6 mice were immunized with the specific antigen MOG35−55, and then the egg extracts were utilized for prophylaxis and/or treatment. Clinical symptoms and other relevant parameters were assessed daily. The mRNA expression of transforming growth factor‐β (TGF‐β), interleukin‐10 (IL‐10), IL‐6, IL‐23 and IL‐17 were assessed with a real‐time polymerase chain reaction technique. Furthermore, secretion of TGF‐β and IL‐17 cytokines were determined by enzyme‐linked immunosorbent assay. Data indicated that clinical symptoms in prophylaxis and treatment groups were decreased significantly in comparison with the untreated control group (p < .001). Our results showed a significant decrease in IL‐17, as well as an increase in TGF‐β cytokine in the treatment group compared to the EAE control group (p < .01). Furthermore, in the prophylaxis and treatment groups, the mRNA expression of disease‐associated cytokines decreased and the mRNA expression of the anti‐inflammatory cytokines increased. In this study, the D. dendriticum egg ameliorates the clinical symptoms of the EAE model through the modulation of related cytokines of Th17 and Treg cells. Therefore, using this parasite egg could be a new treatment for MS.

show abstract

The Role of Dicrocoelium dendriticum Egg Antigen in Colitis: A Molecular, Pathological and Serological Study in an Experimental Model of C57BL/6 Mice

Eilakinezhad,

Mighani,

Khazaei

et al. 2024

Acta Parasit.

View full text Add to dashboard Cite

Dicrocoelium ova can block the induction phase of experimental autoimmune encephalomyelitis

Cited by 5 publications

References 37 publications

Could Dicrocoelium dendriticum eggs play a potential immunotherapeutic role experimental colitis?

Could Dicrocoelium dendriticum eggs play a potential immunotherapeutic role experimental colitis?

Evaluation of the immunoregulatory effect of Dicrocoelium dendriticum eggs on inflammatory and anti‐inflammatory cytokines in EAE model

The Role of Dicrocoelium dendriticum Egg Antigen in Colitis: A Molecular, Pathological and Serological Study in an Experimental Model of C57BL/6 Mice

Contact Info

Product

Resources

About