DIRC3and close toNABP1Genetics Polymorphisms correlated with Prognostic Survival in Patients with Laryngeal Squamous Cell Carcinoma

Abstract

Laryngeal squamous cell carcinoma (LSCC) is one of the most common and aggressive malignancies in the upper digestive tract that has a high mortality rate and a poor prognosis. Prognostic factors were determined through multivariate Cox regression analysis. The overall survival rates were calculated by the Kaplan-Meier method. The SPSS statistical software package version 17.0 (SPSS Inc., Chicago, IL, USA) was used for all analyses. Median follow-up was 38 (range 3-122) months and the median survival time was 48 months. We adjusted to confounding factors (total laryngectomy, poor differentiation, T3-T4 stage, N1-N2 stage, III-IV TNM stage) into multivariate Cox proportional hazards model, we confirmed rs11903757 GT genotype (HR = 2.036; 95% CI, 1.071 -3.872; p = 0.030) and rs966423 TT genotype (HR = 11.677; 95% CI, 3.901 -34.950; p = 0.000) were significantly correlated with prognostic survival of patients with LSCC compared with rs11903757 TT genotype and rs966423 CC genotype, respectively. Our research provided new evidence for patients with LSCC, it seemed to be the first that demonstrated rs11903757 GT genotype on chromosome 2q32.3 close to NABP1 and rs966423 TT genotype in the intron region of DIRC3 on chromosome 2q35 predict poor prognostic survival in patients with LSCC.Keywords: DIRC3, LSCC, NABP1, overall survival, prognostic factors LSCC 3-5 . Despite significant advances in surgery and radio therapeutic techniques, as well as the attempt to use new chemotherapy drugs, the 5-year relative survival rates for patients with LSCC have not markedly improved. The mortality rate of LSCC is still high with 1.2 cases per 100,000 persons 6 . Although great progress has been achieved in the study on laryngeal cancer, there are no ideal biomarkers for the determination of prognosis and guidance of treatment in laryngeal cancer patients.Presently, much work is focused on the identification of useful biologic and molecular markers in the diagnosis and therapy of LSCC 7, 8 . In recent years, scholars have proposed that the combined expression of multiple genes may serve as a satisfactory marker for prognostic prediction. As tissue microarray technology and proteomics develop, more and more metastasis suppressor genes have been found. Exploring relations among anti-metastasis genes plays an important part in the prognostic prediction and treatment of LSCC. Therefore, it is imperative to find novel molecular markers underlying the tumorigenesis of LSCC for early diagnosis and prognostic assessment.We undertook a retrospective analysis of data from a prospective longitudinal study of 170 patients over an extended time period (2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012)(2013) to examine the epidemiology of laryngeal squamous cell carcinoma (LSCC) with regard to age, laryngectomy, neck dissection, tumor differentiation, T-stage, N-stage, TNM stage and treatment modality.Meanwhile, in analyzing possible genetic polymorphisms associated with the susceptibility and prognostic evaluation of SLCC, we hoped...