Discopersicus n. gen., a New Member of the Family Tylenchidae Orley, 1880 with Detailed SEM Study on Two Known Species of the Genus Discotylenchus Siddiqi, 1980 (Nematoda; Tylenchidae) from Iran

Abstract

Discopersicus iranicus n. gen., n. comb., previously described from Iran as a new species under the genus Discotylenchus, is illustrated using light microscope and scanning electron microscope (SEM) observations and further studied using molecular characters. SEM studies revealed the newly proposed genus has oblique amphidial apertures on the lateral sides of the lip region. SEM images are also provided for two species of Discotylenchus, namely D. discretus and D. brevicaudatus, as the first SEM study of the genus. These results confirmed longitudinal amphidial aperture type on lateral sides of the lip region in genus Discotylenchus, as noted by Siddiqi while erecting the genus with D. discretus as the type species. Molecular phylogenetic analyses using partial small subunit (SSU) and large subunit (LSU) rDNA sequences revealed the affinity of the genus Discopersicus n. gen. with members of the subfamily Boleodorinae, as supported by morphological characters (mainly, the oblique amphidial opening).

3D printing in zoological systematics: Integrative taxonomy ofLabrys chinensisgen. nov., sp. nov. (Nematoda: Tylenchomorpha)

“…Currently, 44 genera are recognized in the family Tylenchidae: 42 genera as listed by Geraert (), one new genus recently described by Yaghoubi et al. (), and one by the present study. In this study, integrative approaches were used to describe Labrys chinensis gen. nov., sp.…”

Morphological and molecular characterization of Filenchus pseudodiscus n. sp. from east Golestan province, north Iran; with an updated phylogeny of Malenchus Andrássy, 1968 (Tylenchomorpha: Tylenchidae)

“…It is however proposed that in lacking SEM data, the species with typological similarities to both genera Filenchus and Discotylenchus and having a disc-like structure, need(s) to be compared with species under both genera. SEM studies (in integration with molecular data) of the tylenchid populations having disc-like differentiation in frontal end plus additional characteristics gave rise to establishing Tylenchidae genera in recent years (Yaghoubi et al, 2016;Qing and Bert, 2018;Gharahkhani et al, 2020). Complementary SEM studies were also proposed for clarification the status of some species apparently erroneously assigned to other genera (see Panahandeh et al, 2019a for the status of Discotylenchus lorestanensis Mehrabian et al, 2017).…”

“…The genus Discotylenchus was originally established based upon traditional criteria, and SEM and molecular data of the type populations of its six known species sensu Geraert ( 2008) are not available. The only available SEM images of the genus correspond to an Iranian population of Discotylenchus discretus (Siddiqi, 1980) by Yaghoubi et al (2016). The molecular phylogenetic studies on Filenchus spp.…”

Morphological and Molecular Characterization of Labrys filiformis n. sp. (Rhabditida: Tylenchidae) from Iran

“…According to a comprehensive review of the family Tylenchidae by Geraert (2008), the family includes 42 valid genera. Recently, two other monotypic genera were added, i.e., Discopersicus (Yaghoubi et al, 2016) from Iran, and Labrys (Qing and Bert, 2018) from China with L. chinensis (Qing and Bert, 2018) as its type species (Qing and Bert, 2018). The latter genus is mainly characterized by having a unique offset labial plate, tapering toward both tips and detached from the adjacent cuticle in scanning electron microscopy (SEM) images, protruding lips under LM, laterally elongate amphidial apertures, two incisures in lateral field, delicate stylet, its shaft two times longer than the cone, elongate fusiform weakly developed median bulb having a distinct weakly sclerotized valvular apparatus, wide excretory pore with heavily sclerotized duct, a round spacious postvulval uterine sac (PUS) and spicules with sharp protrusion (Qing and Bert, 2018).…”