<i>Dolosigranulum pigrum</i> causing biomaterial‐associated arthritis

Johnsen, Bjørn Odd; Rønning, Else Johanne; Onken, Annette; Figved, Wender; Jenum, Pål A.

doi:10.1111/j.1600-0463.2010.02697.x

Cited by 13 publications

(8 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1). These findings are consistent with there being only a few isolated reports of D. pigrum growth in samples from different types of infections (77)(78)(79)(80)(81)(82). Of these, the repeated detection of D.…”

Section: Discussionsupporting

confidence: 91%

Genomic Stability and Genetic Defense Systems inDolosigranulum pigruma Candidate Beneficial Bacterium from the Human Microbiome

Ramos

Brugger

Escapa³

et al. 2021

Preprint

View full text Add to dashboard Cite

Dolosigranulum pigrum is positively associated with indicators of health in multiple epidemiological studies of human nasal microbiota. Knowledge of the basic biology of D. pigrum is a prerequisite for evaluating its potential for future therapeutic use; however, such data are very limited. To gain insight into D. pigrum's chromosomal structure, pangenome and genomic stability, we compared the genomes of 28 D. pigrum strains that were collected across 20 years. Phylogenomic analysis showed closely related strains circulating over this period and closure of 19 genomes revealed highly conserved chromosomal synteny. Gene clusters involved in the mobilome and in defense against mobile genetic elements (MGEs) were enriched in the accessory genome versus the core genome. A systematic analysis for MGEs identified the first candidate D. pigrum prophage and insertion sequence. A systematic analysis for genetic elements that limit the spread of MGEs, including restriction modification (RM), CRISPR-Cas, and deity-named defense systems, revealed strain-level diversity in host defense systems that localized to specific genomic sites including one RM system hotspot. Analysis of CRISPR spacers pointed to a wealth of MGEs against which D. pigrum defends itself. These results reveal a role for horizontal gene transfer and mobile genetic elements in strain diversification while highlighting that in D. pigrum this occurs within the context of a highly stable chromosomal organization protected by a variety of defense mechanisms.

show abstract

Section: Discussionsupporting

confidence: 91%

Genomic Stability and Genetic Defense Systems inDolosigranulum pigruma Candidate Beneficial Bacterium from the Human Microbiome

Ramos

Brugger

Escapa³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…It is unclear whether these strains were contaminants or pathogenic contributors (60). However, D. pigrum strains are infrequently associated with disease (61)(62)(63)(64)(65)(66)(67)(68). These 11 D. pigrum strains did not appear to encode potential virulence factors (see section III in Text S2 in the supplemental material), which is consistent with D. pigrum acting primarily as a mutualistic species of humans.…”

Section: Discussionmentioning

confidence: 67%

Dolosigranulum pigrum Cooperation and Competition in Human Nasal Microbiota

Brugger

Eslami²,

Pettigrew

et al. 2020

mSphere

View full text Add to dashboard Cite

Multiple epidemiological studies identify Dolosigranulum pigrum as a candidate beneficial bacterium based on its positive association with health, including negative associations with nasal/nasopharyngeal colonization by the pathogenic species Staphylococcus aureus and Streptococcus pneumoniae. Using a multipronged approach to gain new insights into D. pigrum function, we observed phenotypic interactions and predictions of genomic capacity that support the idea of a role for microbe-microbe interactions involving D. pigrum in shaping the composition of human nasal microbiota. We identified in vivo community-level and in vitro phenotypic cooperation by specific nasal Corynebacterium species. Also, D. pigrum inhibited S. aureus growth in vitro, whereas robust inhibition of S. pneumoniae required both D. pigrum and a nasal Corynebacterium together. D. pigrum l-lactic acid production was insufficient to account for these inhibitions. Genomic analysis of 11 strains revealed that D. pigrum has a small genome (average 1.86 Mb) and multiple predicted auxotrophies consistent with D. pigrum relying on its human host and on cocolonizing bacteria for key nutrients. Further, the accessory genome of D. pigrum harbored a diverse repertoire of biosynthetic gene clusters, some of which may have a role in microbe-microbe interactions. These new insights into D. pigrum’s functions advance the field from compositional analysis to genomic and phenotypic experimentation on a potentially beneficial bacterial resident of the human upper respiratory tract and lay the foundation for future animal and clinical experiments. IMPORTANCE Staphylococcus aureus and Streptococcus pneumoniae infections cause significant morbidity and mortality in humans. For both, nasal colonization is a risk factor for infection. Studies of nasal microbiota identify Dolosigranulum pigrum as a benign bacterium present when adults are free of S. aureus or when children are free of S. pneumoniae. Here, we validated these in vivo associations with functional assays. We found that D. pigrum inhibited S. aureus in vitro and, together with a specific nasal Corynebacterium species, also inhibited S. pneumoniae. Furthermore, genomic analysis of D. pigrum indicated that it must obtain key nutrients from other nasal bacteria or from humans. These phenotypic interactions support the idea of a role for microbe-microbe interactions in shaping the composition of human nasal microbiota and implicate D. pigrum as a mutualist of humans. These findings support the feasibility of future development of microbe-targeted interventions to reshape nasal microbiota composition to exclude S. aureus and/or S. pneumoniae.

show abstract

“… 26 D. pigrum has been associated with nosocomial pneumonia and septicemia, 27 synovitis, 28 acute cholecystitis accompanied by acute pancreatitis, 29 and biomaterial-associated arthritis. 30 Because of the increasing prevalences of multidrug-resistant S. aureus and other nasal commensal bacteria, as well as their propensities for community spread, there is a need for emphasis of standard practices to minimize their transmission during clinical training, which can help to reduce the risks of community- or hospital-acquired infections.…”

Section: Discussionmentioning

confidence: 99%

Prevalences and characteristics of cultivable nasal bacteria isolated from preclinical medical students

Zhou

Sun

et al. 2020

J Int Med Res

View full text Add to dashboard Cite

Objectives Commensal bacteria in the nasal cavity may act as opportunistic pathogens that cause infections under certain conditions. Screening for commensal bacteria in the nasal cavity may aid in understanding their roles in microbiota balance and preventing potential infections. Methods Nasal samples were collected from healthy preclinical medical students and used to inoculate various bacterial culture media, by means of the WaspLab microbiology automated system. Bacterial colonies were then identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Antibiotic resistance phenotypes of Staphylococcus aureus were determined by antibiotic susceptibility tests. Results In total, 549 bacterial strains were isolated from 161 participants. These strains included the following genera: Staphylococcus, Streptococcus, Corynebacterium, Dolosigranulum, Bacillus, Micrococcus, Haemophilus, Neisseria, Moraxella, Pseudomonas, and members of Enterobacteriaceae (e.g., Escherichia, Klebsiella, Citrobacter, Enterobacter, and Serratia). Approximately 25.5% of students were carriers of S. aureus; most S. aureus isolates were resistant to penicillin, erythromycin, and clindamycin. The prevalence of methicillin-resistant S. aureus in nasal samples was 4.3%. Conclusions A diverse group of nasal commensal bacteria inhabited our population of healthy volunteers. These data can improve comprehension of the potential roles of these nasal commensal bacteria in regulating microbiota balance and promoting or mitigating potential future infections.

show abstract

Dolosigranulum pigrum causing biomaterial‐associated arthritis

Cited by 13 publications

References 7 publications

Genomic Stability and Genetic Defense Systems inDolosigranulum pigruma Candidate Beneficial Bacterium from the Human Microbiome

Genomic Stability and Genetic Defense Systems inDolosigranulum pigruma Candidate Beneficial Bacterium from the Human Microbiome

Dolosigranulum pigrum Cooperation and Competition in Human Nasal Microbiota

Prevalences and characteristics of cultivable nasal bacteria isolated from preclinical medical students

Contact Info

Product

Resources

About