<i>Drosophila</i>architectural protein CTCF is not essential for fly survival and is able to function independently of CP190

Kyrchanova, Olga; Klimenko, Natalia; Postika, Nikolay; Bonchuk, Artem; Zolotarev, Nicolay; Maksimenko, Oksana; Georgiev, Pavel

doi:10.1101/2021.05.31.446447

Cited by 3 publications

(2 citation statements)

References 94 publications

(77 reference statements)

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“…partially ameliorated by introducing a mutation in the fly CTCF gene (GE24185) [60,62]. The GE24185 mutation in a wt Fab-7 background disrupts Abd-B expression (figure 3).…”

Section: The Blocking Function F8 209 Can Interfere With Fab-7 Depend...mentioning

confidence: 99%

“…To introduce gy + F7 into the su(Hw) − null background, we combined su(Hw)v/su(Hw) e04061 and gy + F7 as described previously [71]. To introduce F8 209 + F7 into the dctcf null background, we combined the GE24185 mutation and F8 209 + F7 as described previously [60,62]. royalsocietypublishing.org/journal/rsob Open Biol.…”

Section: Generation Of Transgenic Lines Carrying Different Deletions ...mentioning

confidence: 99%

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Boundary bypass activity in theabdominal-Bregion of theDrosophilabithorax complex is position dependent and regulated

et al. 2023

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Expression of Abdominal-B ( Abd-B ) in abdominal segments A5 – A8 is controlled by four regulatory domains, iab-5–iab-8 . Each domain has an initiator element (which sets the activity state), elements that maintain this state and tissue-specific enhancers. To ensure their functional autonomy, each domain is bracketed by boundary elements ( Mcp , Fab-7 , Fab-7 and Fab-8 ). In addition to blocking crosstalk between adjacent regulatory domains, the Fab boundaries must also have bypass activity so the relevant regulatory domains can ‘jump over’ intervening boundaries and activate the Abd-B promoter. In the studies reported here we have investigated the parameters governing bypass activity. We find that the bypass elements in the Fab-7 and Fab-8 boundaries must be located in the regulatory domain that is responsible for driving Abd-B expression. We suggest that bypass activity may also be subject to regulation.

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“…partially ameliorated by introducing a mutation in the fly CTCF gene (GE24185) [60,62]. The GE24185 mutation in a wt Fab-7 background disrupts Abd-B expression (figure 3).…”

Section: The Blocking Function F8 209 Can Interfere With Fab-7 Depend...mentioning

confidence: 99%

Section: Generation Of Transgenic Lines Carrying Different Deletions ...mentioning

confidence: 99%

Boundary bypass activity in theabdominal-Bregion of theDrosophilabithorax complex is position dependent and regulated

et al. 2023

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The N-Terminal Part of Drosophila CP190 Is a Platform for Interaction with Multiple Architectural Proteins

Golovnin,

Melnikova,

Babosha

et al. 2023

IJMS

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CP190 is a co-factor in many Drosophila architectural proteins, being involved in the formation of active promoters and insulators. CP190 contains the N-terminal BTB/POZ (Broad-Complex, Tramtrack and Bric a brac/POxvirus and Zinc finger) domain and adjacent conserved regions involved in protein interactions. Here, we examined the functional roles of these domains of CP190 in vivo. The best-characterized architectural proteins with insulator functions, Pita, Su(Hw), and dCTCF, interacted predominantly with the BTB domain of CP190. Due to the difficulty of mutating the BTB domain, we obtained a transgenic line expressing a chimeric CP190 with the BTB domain of the human protein Kaiso. Another group of architectural proteins, M1BP, Opbp, and ZIPIC, interacted with one or both of the highly conserved regions in the N-terminal part of CP190. Transgenic lines of D. melanogaster expressing CP190 mutants with a deletion of each of these domains were obtained. The results showed that these mutant proteins only partially compensated for the functions of CP190, weakly binding to selective chromatin sites. Further analysis confirmed the essential role of these domains in recruitment to regulatory regions associated with architectural proteins. We also found that the N-terminal of CP190 was sufficient for recruiting Z4 and Chromator proteins and successfully achieving chromatin opening. Taken together, our results and the results of previous studies showed that the N-terminal region of CP190 is a platform for simultaneous interaction with various DNA-binding architectural proteins and transcription complexes.

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Developmental and Housekeeping Genes: Two Types of Genetic Organization in the Drosophila Genome

Zhimulev,

Vatolina,

Levitsky

et al. 2024

IJMS

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We developed a procedure for locating genes on Drosophila melanogaster polytene chromosomes and described three types of chromosome structures (gray bands, black bands, and interbands), which differed markedly in morphological and genetic properties. This was reached through the use of our original methods of molecular and genetic analysis, electron microscopy, and bioinformatics data processing. Analysis of the genome-wide distribution of these properties led us to a bioinformatics model of the Drosophila genome organization, in which the genome was divided into two groups of genes. One was constituted by 65, in which the genome was divided into two groups, 62 genes that are expressed in most cell types during life cycle and perform basic cellular functions (the so-called “housekeeping genes”). The other one was made up of 3162 genes that are expressed only at particular stages of development (“developmental genes”). These two groups of genes are so different that we may state that the genome has two types of genetic organization. Different are the timings of their expression, chromatin packaging levels, the composition of activating and deactivating proteins, the sizes of these genes, the lengths of their introns, the organization of the promoter regions of the genes, the locations of origin recognition complexes (ORCs), and DNA replication timings.

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Drosophilaarchitectural protein CTCF is not essential for fly survival and is able to function independently of CP190

Cited by 3 publications

References 94 publications

Boundary bypass activity in theabdominal-Bregion of theDrosophilabithorax complex is position dependent and regulated

Boundary bypass activity in theabdominal-Bregion of theDrosophilabithorax complex is position dependent and regulated

The N-Terminal Part of Drosophila CP190 Is a Platform for Interaction with Multiple Architectural Proteins

Developmental and Housekeeping Genes: Two Types of Genetic Organization in the Drosophila Genome

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