<i>Eimeria tenella</i>
            : Cultivation of the Asexual Stages in Cultured Animal Cells

Patton, W. H.

doi:10.1126/science.150.3697.767

Cited by 66 publications

(30 citation statements)

References 9 publications

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“…There was no initiation of second generation schizogony. These observations are similar to those described by others using MDBK cells (Patton 1965;Schmatz et al 1986;Chai et al 1989;Kogut and Lange 1989). Also, at 84 h p.i., the first intracellular merozoites were observed.…”

Section: Resultssupporting

confidence: 93%

“…In vitro culture has become a useful tool by enabling us to understand aspects of their lifecycle that could not otherwise be studied (McDougald 1978). Patton (1965) first described the in vitro cultivation of this parasite in Madin-Darby kidney (MDBK) cells in which he obtained first stage, asexual development. The complete lifecycle has been propagated in primary chicken kidney cells (Doran 1970;Hofmann and Raether 1990;Zhang et al 1997) but in a modified form.…”

Section: Introductionmentioning

confidence: 99%

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Comparative development of Eimeria tenella (Apicomplexa) in host cells in vitro

Tierney

Mulcahy

2003

Parasitology Research

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Attempts to propagate Eimeria tenella in cell culture over the years have met with limited success. The host cell type is an important parameter in such cultures. This study assessed the ability of different host cell lines to support E. tenella infection in vitro. The initial development in cell lines and the effects of incubation at 37 degrees C and 41 degrees C on the host-parasite relationship was investigated. Eleven cell lines were seeded into 96 well plates and incubated at 37 degrees C in 5% CO(2) to reach confluency. Sporozoites of E. tenella were inoculated into wells and allowed to invade at 37 degrees C and 41 degrees C. Intracellular parasite development was quantified using (3)H-uracil incorporation. All cell lines facilitated parasite invasion and development. The MDBK cell line supported the highest degree of E. tenella development. A time-course study was undertaken to look at this host cell-parasite relationship during infection.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Comparative development of Eimeria tenella (Apicomplexa) in host cells in vitro

Tierney

Mulcahy

2003

Parasitology Research

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“…The development of SZ in the DF-1 system is recognized for its marked similarity to that in CEV-1, which has been extensively used over the past decades for E. tenella research (Danforth et al 1994;Peroval et al 2006;Labbe et al 2005). Moreover, DF-1 supports a higher degree of E. tenella invasion and development than the other commonly used cell line, Madin-Darby bovine kidney cells (Patton 1965; data not shown).…”

Section: Discussionmentioning

confidence: 71%

Identification and partial characterization of a serine protease inhibitor (serpin) of Eimeria tenella

et al. 2011

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Serine protease inhibitors (serpins) mediate many biological processes, including immune responses to pathogenic infection. In this study, a member of the serpin superfamily was identified from the common poultry parasite Eimeria tenella by expressed sequence tag analysis and the rapid amplification of cDNA ends technique. The full-length cDNA was 1,918 bp and had an open reading frame of 1,248 bp encoding a polypeptide of 415 amino acids with the theoretical isoelectric point of 5.26 and predicted molecular weight of 45.5 kDa. Real-time quantitative PCR analysis revealed that the serpin gene was expressed at higher levels in sporozoites than in the other developmental stages (unsporulated oocysts, sporulated oocysts, and second-generation merozoites). The sequence encoding the mature protein was amplified by PCR, cloned into the pET28(a) vector, and expressed in Escherichia coli. Specific antiserum generated against the recombinant protein was prepared and used to determine invasion inhibition capacity and localization; the results suggested that the serpin may play an important role in invasion and survival of the sporoziotes in the host.

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“…Coccidia are ubiquitous sporozoan parasites that are of significance in the intensive rearing of poultry. The constant demand for new anticoccidial drugs and the number of drugs that have been introduced are explained by the commercial importance of coccidiosis, the early establishment of efficient laboratory models for testing anticoccidial activity in vitro and in vivo (28,29), and the high rate of resistance development in the field (7). We were interested in learning to what extent anticoccidial drugs might be a source of potential antitoxo-* Corresponding author.…”

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confidence: 99%

Toxoplasma gondii: susceptibility and development of resistance to anticoccidial drugs in vitro

Ricketts

Pfefferkorn

1993

Antimicrob Agents Chemother

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Anticoccidial drugs were evaluated for activity and for the development of resistance in a model of Toxoplasma gondii growing in human fibroblast cultures. Of 13 anticoccidial drugs tested, 9 had selective antitoxoplasma activity (50% inhibitory concentration, in micrograms per milliliter): decoquinate (0.005), arprinocid-N-oxide (0.015), robenidine (0.03), the aryl triazine CP-25,415 (0.2), toltrazuril (0.4), clopidol (1), dinitolmide (Zoalene; Dow) (10), and the carboxylic acid ionophores monensin (0.001) and salinomycin (0.04). Glycarbylamide, amprolium, nicarbazin, and the 6-(p-bromophenoxy)-7-chloro analog of halofuginone (Stenorol; Roussel-UCLAF) (CP-63,567) were toxic for the fibroblasts. Since Eimeria tenella has a similar drug susceptibility profile, anticoccidial drugs can be viewed as a potential source of new antitoxoplasma therapies. The development of resistance has limited the usefulness of most of these drugs as anticoccidial agents; in coccidia, resistance to all except the ionophores occurs readily in vivo. We explored the development of resistance in T. gondii by attempting to select mutants in vitro from parasites mutagenized with ethylnitrosourea. Mutants that had 20-to 50-fold-reduced susceptibility to decoquinate, arprinocid-N-oxide, and CP-25,415 were obtained. Ionophore-resistant T. gondii mutants were also selected in vitro; however, there was only a twofold difference in susceptibility between these mutants and the wild type. For three drugs (clopidol, robenidine, and toltrazuril), we were unable to select resistant mutants. For experimental anticoccidial drugs, there is currently no in vitro method for assessing the risk of development of resistance in Eimeria species. Our results suggest that T. gondii may offer a useful surrogate for this assessment.

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Eimeria tenella : Cultivation of the Asexual Stages in Cultured Animal Cells

Cited by 66 publications

References 9 publications

Comparative development of Eimeria tenella (Apicomplexa) in host cells in vitro

Comparative development of Eimeria tenella (Apicomplexa) in host cells in vitro

Identification and partial characterization of a serine protease inhibitor (serpin) of Eimeria tenella

Toxoplasma gondii: susceptibility and development of resistance to anticoccidial drugs in vitro

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