Eleutherine americana extract inhibits adherence to and invasion of Caco‐2 cells by commonly contaminated Campylobacter spp. in food

Abstract

Eleutherine americana, a well‐known Asian medicinal herb, has been claimed for many biological activities. In this study, potential of E. americana ethanolic extract against adhesion and invasion of Caco‐2 cells by commonly contaminated Campylobacter spp. was evaluated. MIC50 values of E. americana extract against 39 enteritis patient and 26 chicken isolates were 125 µg/ml. Two models were set up: Model I, Prevention: Preincubation of E. americana extract with campylobacters before adding to Caco‐2 cells. Adhesion to and invasion of Caco‐2 cells by campylobacters were completely inhibited within 12 hr. Model II, Treatment: Preincubation of the organisms with Caco‐2 cells before adding the extract. Adding the extract after the bacteria were mixed with Caco‐2 cells resulted in moderate effect on adhesion, but complete prevention of penetration of the organisms into Caco‐2 cells was observed. The extract at the highest concentration tested (250 μg/ml) demonstrated no cytotoxicity toward Caco‐2 cells. Practical applications Eleutherine americana bulb extract could be applied as an effective food preservative to control campylobacter contamination at initial stage in food chain. In addition, the extract provides a curative approach to campylobacter infections.

Eleutherine bulbosa (Mill.) Urb. Bulb: Review of the Pharmacological Activities and Its Prospects for Application

“…Sirirak et al [43] evaluated the anti-adhesive and invasion properties of Campylobacter spp. on colon cancer cells (Caco-2) using the E. americana bulb extract.…”