2009
DOI: 10.1128/jcm.00202-09
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emm1/Sequence Type 28 Strains of Group A Streptococci That ExpresscovRat Early Stationary Phase Are Associated with Increased Growth and Earlier SpeB Secretion

Abstract: Streptococcus pyogenes (group A streptococcus [GAS]) is a versatile human pathogen, and emm1/sequence type 28 (ST28) is the most frequently isolated type from GAS infections. The emm1/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation is one of the important regulatory mechanisms in GAS, which controls gene expression at restricted phases of growth. CovRS, a two-component regulatory system, is considered the regulator of streptococcal pyrogenic … Show more

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Cited by 22 publications
(21 citation statements)
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“…Group A Streptococcus strain A20 (wild type strain) is an emm1 -type strain and has been previously described (Chiang-Ni et al, 2009). Strain AP3 was provided by Prof. Jiunn Jong Wu (Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Group A Streptococcus strain A20 (wild type strain) is an emm1 -type strain and has been previously described (Chiang-Ni et al, 2009). Strain AP3 was provided by Prof. Jiunn Jong Wu (Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan).…”
Section: Methodsmentioning
confidence: 99%
“…Group A Streptococcus genomic DNA extraction, RNA extraction, and reverse transcription were performed as described previously (Chiang-Ni et al, 2009). Real-time PCR reactions were performed in a 20 μl mixture containing 1 μl of cDNA, 0.8 μl of primers (10 μM), and 10 μl of SensiFAST TM SYBR Lo-ROX pre-mixture (Bioline Ltd, London, UK) according to the manual.…”
Section: Methodsmentioning
confidence: 99%
“…GAS strains A-20 (M1T1, emm1/ST28), GAS 602 (M1, emm1/ST28), SF370 (M1, emm1/ST28), GAS 113 (emm4/ST391), GAS 133 (emm4/ST39), GAS 261 (emm12/ST36), GAS 747 (emm12/ST465) and NZ131 (emm49/ ST424) have been described previously (Chiang-Ni et al, 2009). All GAS strains were grown in tryptic soy broth (Becton Dickinson) supplemented with 0.5 % yeast extract (TSBY).…”
Section: Methodsmentioning
confidence: 99%
“…Western blotting to detect SpeB in bacterial culture supernatant was performed as described previously (Chiang-Ni et al, 2009). Briefly, 300 ml bacterial culture supernatant was mixed with 1.2 ml acetone and incubated at 220 uC for 1 h to precipitate the total protein.…”
mentioning
confidence: 99%
“…Mucoid GAS colony phenotype is usually ascribed to an increase in hyaluronan capsule [13,22] and accordingly, a significant difference in hyaluronan capsule production was observed between mucoid and non-mucoid colonies cultured from lymph node (Figure 5(c)). To determine if hypermucoid strains were accounted for by mutations known to be selected in vivo , amplicons of covRS and rocA [13,23,24], were sequenced from 10 mucoid lymph node colonies, but mutations were not detected. To further determine the basis for hyper-encapsulation, DNA from 5 mucoid colonies cultured from spleen, and 5 mucoid colonies from the draining inguinal lymph node of a single mouse was extracted, subjected to whole genome sequencing, and compared with the parent strain genome sequence.…”
Section: Resultsmentioning
confidence: 99%