2008
DOI: 10.1128/aem.01831-07
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Endomicrobia” and Other Bacteria Associated with the Hindgut ofDermolepida albohirtumLarvae

Abstract: Symbiotic bacteria residing in the hindgut chambers of scarab beetle larvae may be useful in paratransgenic approaches to reduce larval root-feeding activities on agricultural crops. We compared the bacterial community profiles associated with the hindgut walls of individual Dermolepida albohirtum third-instar larvae over 2 years and those associated with their plant root food source among different geographic regions. Denaturing gradient gel electrophoresis analysis was used with universal and Actinobacteria-… Show more

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Cited by 17 publications
(37 citation statements)
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“…The resulting PCR products were cloned into the pGEM-T Easy vector (Promega), and for each product, four clones from each infected cell line were randomly picked and sequenced. The presence of wMelPop and no other contaminating bacteria in cell lines was verified by denaturing gradient gel electrophoresis using a general primer set targeting eubacterial 16S rRNA genes (F-968-GC and R-1401) (25) by methods described previously (29).…”
Section: Methodsmentioning
confidence: 99%
“…The resulting PCR products were cloned into the pGEM-T Easy vector (Promega), and for each product, four clones from each infected cell line were randomly picked and sequenced. The presence of wMelPop and no other contaminating bacteria in cell lines was verified by denaturing gradient gel electrophoresis using a general primer set targeting eubacterial 16S rRNA genes (F-968-GC and R-1401) (25) by methods described previously (29).…”
Section: Methodsmentioning
confidence: 99%
“…Members of the hindgut community that are associated with larvae of the Australian scarab pest, Dermolepida albohirtum (32), and the European scarab pest, Melolontha melolontha (15), are consistently found across the geographical distribution of the larval host. These commonly found gut bacteria are possible candidates in a biotechnological approach to control the feeding damage caused by scarab larvae in agricultural crops (32). This could be achieved by genetically engineering gut bacteria to express antifeeding compounds that target larvae and applying them to soil containing the pest.…”
mentioning
confidence: 98%
“…Larvae were surface sterilized by immersion in sodium hypochlorite (50 g liter Ϫ1 ) and rinsed with 70% ethanol, and the following procedures were carried out using standard sterile techniques. Larvae were dissected and the hindgut chamber isolated as described previously (32). The hindgut chamber was cut in half and rinsed three times in sterilized water to remove the lumen contents.…”
mentioning
confidence: 99%
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