Escherichia coli survival in plasma‐treated water and in a gas–liquid plasma reactor

Abstract

The effects of post-plasma exposure to deionized water treated by argon-water plasma and to direct plasma treatment of bacteria in a liquid solution of a strain of Escherichia coli, which was independently evolved to resist the bactericidal effects of hydrogen peroxide, were determined. The hydrogen peroxide resistant phenotype of the evolved strain was proportional to the initial cell density. The catalase activity was significantly elevated in the evolved strain as compared with the wild-type strain, and the evolved strain resisted the bactericidal effects of plasma-treated water (post-plasma exposure) as compared with the wild-type strain. The evolved strain remained viable after flowing through the plasma reactor and lysed less when exposed to (nonplasma control) hydrogen peroxide solutions as compared with the wild-type cells.

Effect of air, O2, Ar, and N2 plasma‐activated water on mildewing activity of moldy pathogen of Gannan navel oranges

“…$${{\rm{H}}}_{2}{{\rm{O}}}_{2}$$ is a key species of microbial inactivation, which can destroy the integrity of microbial membranes and lead to cell rupture. [ 60 ] Wu et al [ 61 ] also found that the combined action of $${{\rm{H}}}_{2}{{\rm{O}}}_{2}$$ and reactive radicals such as OH contribute to microbial inactivation. Therefore, the higher the concentration of $${{\rm{H}}}_{2}{{\rm{O}}}_{2}$$ in PAW, the better the inactivation effect on microorganisms.…”

Controlling plasma‐activated solution chemistry for targeted cancer cell death

“…The absorption spectra can be used to estimate the RONS concentrations. [ 46 ] Figure 8 shows the effect of the working gas on the UV absorption spectra of different biological solutions. It should be noted that RPMI 1640 is measured after a 200‐fold dilution.…”

Investigation of the chemical characteristics and anticancer effect of plasma‐activated water: The effect of liquid temperature

“…[1][2][3][4][5] It is well known that after plasma ignition, many chemical reactions can be achieved at plasma-liquid interfaces, [6][7][8] inducing abundant primary and secondary reactive oxygen and nitrogen species (RONS: ROS and RNS) and turning the ordinary water into plasmaactivated water (PAW). [9,10] PAW has emerged as a promising technique in dermatology, [11] sterilization, [12] and cancer therapy [13] due to the superiority of flexible production, good fluidity, and abundant RONS. [4,14] These RONS in PAW include H 2 O 2 , NO 2 − , OH, O 2 − , ONOO − , and so forth.…”