<i>Ex vivo</i>characterization of normal and adenocarcinoma colon samples by Mueller matrix polarimetry

Ahmad, Iftikhar; Ahmad, Manzoor; Khan, K.; Ashraf, Sumara; Ahmad, Shakil; Ikram, Manzoor

doi:10.1117/1.jbo.20.5.056012

Cited by 80 publications

(39 citation statements)

References 34 publications

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“…A number of applications of Mueller polarimetric imaging are instead based on detecting unstained thin tissue cuts [22,131,144,[158][159][160][161][162] in a transmission geometry. Since Haematoxylin and Eosin (H&E) staining and other staining techniques require time and elaborate sample preparation by a well-trained and experienced pathologist for diagnosis, unstained Mueller microscopy may be used for rapid and low cost pathological inspection [159,160] and instant tissue characterization [136,163]. As shown in Figure 2(b), polarimetric imaging has been utilized to guide scanning mass spectroscopic analysis of a pathological slide of breast cancer tissue to detect suspicious cancerous regions.…”

Section: The Biomedical Applications Of Mueller-polarimetric Imagingmentioning

confidence: 99%

Mueller polarimetric imaging for surgical and diagnostic applications: a review

2017

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Polarization is a fundamental property of light and a powerful sensing tool that has been applied to many areas. A Mueller matrix is a complete mathematical description of the polarization characteristics of objects that interact with light, and is known as a transfer function of Stokes vectors which characterise the state of polarization of light. Mueller polarimetric imaging measures Mueller matrices over a field of view and thus allows for visualising the polarization characteristics of the objects. It has emerged as a promising technique in recent years for tissue imaging, improving image contrast and providing a unique perspective to reveal additional information that cannot be resolved by other optical imaging modalities. This review introduces the basis of the Stokes-Mueller formulism, interpretation methods of Mueller matrices into fundamental polarization properties, polarization properties of biological tissues, and considerations in the construction of Mueller polarimetric imaging devices for surgical and diagnostic applications, including primary configurations, optimization procedures, calibration methods as well as the instrument polarization properties of several widelyused biomedical optical devices. The paper also reviews recent progress in Mueller polarimetric endoscopes and fibre Mueller polarimeters, followed by the future outlook in applying the technique to surgery and diagnostics. Tissue polarization properties convey morphological, micro-structural and compositional information of tissue with great potential for label free characterization of tissue pathological changes. Recent progress in tissue polarimetric imaging and polarization resolved endoscopy paved the way for translation of polarimetric imaging to surgery and tissue diagnosis.

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Section: The Biomedical Applications Of Mueller-polarimetric Imagingmentioning

confidence: 99%

Mueller polarimetric imaging for surgical and diagnostic applications: a review

2017

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“…The near-infrared (NIR) light, on the other hand, penetrates much deeper into the tissue, and is well suited for deep tissue diagnosis [12,[20][21][22]. Further, the reported Mueller-Matrix polarimetries only acquired either the images [7,8] or the optical spectra [9] of the biological tissues alone. In this work, we report on the development of a unique integrated Mueller-Matrix NIR imaging and pointwise Mueller-Matrix spectroscopy system for colonic tissue diagnosis and characterization.…”

Section: Introductionmentioning

confidence: 99%

“…Polarized light imaging/spectroscopy has been comprehensively investigated for tissue diagnosis [6][7][8][9][10][11][12][13][14][15][16][17]. Polarized light implementation offers several compelling advantages as follows: (i) surface and beneath-the-surface detection of biological tissue taken from the tissue depolarization properties [11,15]; (ii) tissue anisotropy analysis through the tissue diattentuation and retardance [13,14]; (3) enhanced tissue diagnosis through the combination of complementary depolarization, diattentuation and retardance of the tissues [9].…”

Section: Introductionmentioning

confidence: 99%

“…Polarized light implementation offers several compelling advantages as follows: (i) surface and beneath-the-surface detection of biological tissue taken from the tissue depolarization properties [11,15]; (ii) tissue anisotropy analysis through the tissue diattentuation and retardance [13,14]; (3) enhanced tissue diagnosis through the combination of complementary depolarization, diattentuation and retardance of the tissues [9]. Among the various polarized light imaging/spectroscopy techniques developed [6][7][8][9][10][11][12][13][14]18], MuellerMatrix polarimetry is capable of measuring the complete polarimetric transfer function [6][7][8][9], known as Mueller-matrix, of the bulk biological tissues which are optically inhomogeneous, birefringent, and absorbing media [19]. Currently, biomedical Mueller-Matrix polarimetry is mostly centered on the use of short visible wavelengths of illumination light that has a limited penetration depth and cannot detect lesions in deeper areas [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Among the various polarized light imaging/spectroscopy techniques developed [6][7][8][9][10][11][12][13][14]18], MuellerMatrix polarimetry is capable of measuring the complete polarimetric transfer function [6][7][8][9], known as Mueller-matrix, of the bulk biological tissues which are optically inhomogeneous, birefringent, and absorbing media [19]. Currently, biomedical Mueller-Matrix polarimetry is mostly centered on the use of short visible wavelengths of illumination light that has a limited penetration depth and cannot detect lesions in deeper areas [8,9]. The near-infrared (NIR) light, on the other hand, penetrates much deeper into the tissue, and is well suited for deep tissue diagnosis [12,[20][21][22].…”

Section: Introductionmentioning

confidence: 99%

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Integrated Mueller-matrix near-infrared imaging and point-wise spectroscopy improves colonic cancer detection

Wang

Zheng

Lin

et al. 2016

Biomed. Opt. Express

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Abstract:We report the development and implementation of a unique integrated Mueller-matrix (MM) near-infrared (NIR) imaging and Muellermatrix point-wise diffuse reflectance (DR) spectroscopy technique for improving colonic cancer detection and diagnosis. Point-wise MM DR spectra can be acquired from any suspicious tissue areas indicated by MM imaging. A total of 30 paired colonic tissue specimens (normal vs. cancer) were measured using the integrated MM imaging and point-wise MM DR spectroscopy system. Polar decomposition algorithms are employed on the acquired images and spectra to derive three polarization metrics including depolarization, diattentuation and retardance for colonic tissue characterization. The decomposition results show that tissue depolarization and retardance are significantly decreased (p<0.001, paired 2-sided Student's t-test, n = 30); while the tissue diattentuation is significantly increased (p<0.001, paired 2-sided Student's t-test, n = 30) associated with colonic cancer. Further partial least squares discriminant analysis (PLS-DA) and leave-one tissue site-out, cross validation (LOSCV) show that the combination of the three polarization metrics provide the best diagnostic accuracy of 95.0% (sensitivity: 93.3%, and specificity: 96.7%) compared to either of the three polarization metrics (sensitivities of 93.3%, 83.3%, and 80.0%; and specificities of 90.0%, 96.7%, and 80.0%, respectively, for the depolarization, diattentuation and retardance metrics) for colonic cancer detection. This work suggests that the integrated MM NIR imaging and point-wise MM NIR diffuse reflectance spectroscopy has the potential to improve the early detection and diagnosis of malignant lesions in the colon. #258157Received 25

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Polarimetric assessment of healthy and radiofrequency ablated porcine myocardial tissue

Ahmad

Gribble

Ikram

et al. 2015

Journal of Biophotonics

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Radiofrequency (RF) ablation offers a potential treatment for cardiac arrhythmia, where properly titrated energy delivered at critical sites can destroy arrhythmogenic foci. The resulting ablation lesion typically consists of a core (coagulative necrosis) surrounded by a rim of mixed viable and non-viable cells. The extent of the RF lesion is difficult to delineate with current imaging techniques. Here, we explore polarization signatures of ten ex-vivo samples from untreated (n = 5) and RF ablated porcine hearts (n = 5), in backscattered geometry through Mueller matrix polarimetry. Significant differences (p < 0.01) in depolarization, ΔT , were observed between the healthy, RF ablated and rim regions. Linear retardance, δ, was significantly lower in the core and rim regions compared to healthy regions (p < 0.05). The results demonstrate a novel application of polarimetry, namely the characterization of RF ablation extent in myocardium, including the visualization of the important lesion rim region. White light photo (top) of porcine myocardium tissue with radiofrequency ablation lesion and corresponding depolarization map (bottom). Depolarization is useful for visualizing the lesion core and rim.

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Ex vivocharacterization of normal and adenocarcinoma colon samples by Mueller matrix polarimetry

Cited by 80 publications

References 34 publications

Mueller polarimetric imaging for surgical and diagnostic applications: a review

Mueller polarimetric imaging for surgical and diagnostic applications: a review

Integrated Mueller-matrix near-infrared imaging and point-wise spectroscopy improves colonic cancer detection

Polarimetric assessment of healthy and radiofrequency ablated porcine myocardial tissue

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