<i>Ex vivo</i> differentiation of umbilical cord blood progenitor cells in the presence of placental conditioned medium

Chivu, Mihaela; Diaconu, Carmen C.; Braşoveanu, Lorelei Irina; Alexiu, Irina; Bleoţu, Coralia; Banceanu, G.; Miscalencu, D; Cernescu, C

doi:10.1111/j.1582-4934.2002.tb00459.x

Cited by 9 publications

(7 citation statements)

References 28 publications

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“…Several studies have reported that conditioned medium (CM) obtained from MSCs contains putative growth factors that accelerate cell division, delay senescence, and enhance cell differentiation (Hakkinen et al. , 2001; Chivu et al. , 2002; Elder, 2002; Torres et al.…”

Section: Resultsmentioning

confidence: 99%

Age‐related changes in mesenchymal stem cells derived from rhesus macaque bone marrow

et al. 2011

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Summary The regeneration potential of mesenchymal stem cells (MSCs) diminishes with advanced age and this diminished potential is associated with changes in cellular functions. This study compared MSCs isolated from the bone marrow of rhesus monkeys (rBMSCs) in three age groups: young (< 5 years), middle (8–10 years), and old (> 12 years). The effects of aging on stem cell properties and indicators of stem cell fitness such as proliferation, differentiation, circadian rhythms, stress response proteins, miRNA expression, and global histone modifications in rBMSCs were analyzed. rBMSCs demonstrated decreased capacities for proliferation and differentiation as a function of age. The production of heat shock protein 70 (HSP70) and heat shock factor 1 (HSF1) were also reduced with increasing age. The level of a core circadian protein, Rev-erb α, was significantly increased in rBMSCs from old animals. Furthermore, analysis of miRNA expression profiles revealed an up-regulation of mir-766 and mir-558 and a down-regulation of mir-let-7f, mir-125b, mir-222, mir-199-3p, mir-23a, and mir-221 in old rBMSCs compare to young rBMSCs. However, there were no significant age-related changes in the global histone modification profiles of the four histone core proteins: H2A, H2B, H3, and H4 on rBMSCs. These changes represent novel insights into the aging process and could have implications regarding the potential for autologous stem cells therapy in older patients.

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Section: Resultsmentioning

confidence: 99%

Age‐related changes in mesenchymal stem cells derived from rhesus macaque bone marrow

et al. 2011

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“…Several features of our experimental design may account for these differences. We supplemented the hADAS cell clones with conditioned media containing putative growth factors which may accelerate cell division, delay senescence, and enhance cell differentiation (Hakkinen et al, 2001;Chivu et al, 2002;Elder, 2002;Torres et al, 2002). Furthermore, our hADAS cell cloning was conducted on low adhesion suspension dishes; adhesion has been found to influence the growth and differentiation potential of stem cells derived from skeletal muscle (Huard et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Clonal analysis of the differentiation potential of human adipose‐derived adult stem cells

Guilak

Lott

Awad

et al. 2005

Journal Cellular Physiology

440

292

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Pools of human adipose-derived adult stem (hADAS) cells can exhibit multiple differentiated phenotypes under appropriate in vitro culture conditions. Because adipose tissue is abundant and easily accessible, hADAS cells offer a promising source of cells for tissue engineering and other cell-based therapies. However, it is unclear whether individual hADAS cells can give rise to multiple differentiated phenotypes or whether each phenotype arises from a subset of committed progenitor cells that exists within a heterogeneous population. The goal of this study was to test the hypothesis that single hADAS are multipotent at a clonal level. hADAS cells were isolated from liposuction waste, and ring cloning was performed to select cells derived from a single progenitor cell. Forty-five clones were expanded through four passages and then induced for adipogenesis, osteogenesis, chondrogenesis, and neurogenesis using lineage-specific differentiation media. Quantitative differentiation criteria for each lineage were determined using histological and biochemical analyses. Eighty one percent of the hADAS cell clones differentiated into at least one of the lineages. In addition, 52% of the hADAS cell clones differentiated into two or more of the lineages. More clones expressed phenotypes of osteoblasts (48%), chondrocytes (43%), and neuron-like cells (52%) than of adipocytes (12%), possibly due to the loss of adipogenic ability after repeated subcultures. The findings are consistent with the hypothesis that hADAS cells are a type of multipotent adult stem cell and not solely a mixed population of unipotent progenitor cells. However, it is important to exercise caution in interpreting these results until they are validated using functional in vivo assays.

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“…A second growth cocktail was based on 20% placental conditioned medium (PCM) (obtained as described before - [14]) and 2% autologous plasma. After three days, total numbers of nucleated and viable cells were determined using trypan blue exclusion test.…”

Section: Expansion Culturesmentioning

confidence: 99%

The comparison of different protocols for expansion of umbilical‐cord blood hematopoietic stem cells

Chivu¹,

Diaconu²,

Bleoţu³

et al. 2004

J Cellular Molecular Medi

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Hematopoiesis is maintained by the activity of multipotent stem cells, which have the dual capacity to self‐renew and to differentiate into all of the blood cell lineages. The major challenge of stem cells based regenerative therapy is to expand ex vivo the primitive compartment to increase transplantable stem cells number. The present study was designed to evaluate several culture systems for in vitro maintenance of umbilical cord blood stem cells. The influences of different growth conditions such as stromal feeder layer, cytokines supplement and placental conditioned medium (PCM) have been evaluated over a relatively short period of time on CD34+ cell expansion and maintenance of clonogenic progenitors. When cells were expanded on feeder layer in the presence of added cytokines and PCM on average a 2.96‐fold increase of CD34+CD71‐ and a 3.13‐fold increase of CD34+HLA‐DR‐ was observed. The total number of colony forming cells (35±2.65) indicated also that the yield of clonogenic progenitors obtained with a combination of all factors was two folds higher than each of these factors alone and ten time above control (3.67± 2.52). In conclusion, the results of our study clearly show that the ex vivo expansion of hematopoietic progenitor cells obtained from human umbilical cord blood is dependent on controlled experimental conditions, which might be helpful when designing culture systems for clinical applications.

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Ex vivo differentiation of umbilical cord blood progenitor cells in the presence of placental conditioned medium

Cited by 9 publications

References 28 publications

Age‐related changes in mesenchymal stem cells derived from rhesus macaque bone marrow

Age‐related changes in mesenchymal stem cells derived from rhesus macaque bone marrow

Clonal analysis of the differentiation potential of human adipose‐derived adult stem cells

The comparison of different protocols for expansion of umbilical‐cord blood hematopoietic stem cells

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