<i>Ex vivo</i> expansion of umbilical cord blood CD34<sup>+</sup> cells in a closed system: a multicentric study

Astori, Giuseppe; Larghero, Jérôme; Bonfini, T.; Giancola, Raffaella; Riti, Maria Di; Rodríguez, Laura; Rodriguez, M.; Mambrini, Giovanni; Bigi, Leonardo; Iacone, A; Marolleau, Jean Pierre; Panzani, I.; Garcı́a, Joan; Querol, Sergio

doi:10.1111/j.1423-0410.2006.00751.x

Cited by 11 publications

(6 citation statements)

References 28 publications

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“…This has meant that UCB has now moved from a biological waste to an ethical, renewable and valuable source of HSCs. The use of this source of HSCs and hematopoietic progenitor cells (HPCs) has rapidly increased over the past 10 years as a result 18. However, the major drawback associated with the use of UCB is the limited number of HSCs within one unit (typically 100 mL, ∼4 × 10 6 CD34 + cells),19 resulting in most of the patients treated with UCB being children 17, 20–22…”

Section: Hematopoietic Stem Cellsmentioning

confidence: 99%

Aquatic toxicity tests with substances that are poorly soluble in water and consequences for environmental risk assessment

Weyman¹,

Rufli

Weltje³

et al. 2012

Environmental Toxicology and Chemistry

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Aquatic toxicity tests with substances that are poorly soluble in water have been conducted using different methods, and estimates of toxicity have varied accordingly. The present study illustrates differences in toxicity values resulting from variation in test designs and solution preparation methods, and offers guidance on the best way to conduct these tests. Consequences for environmental risk assessment and classification are also discussed. The present study mainly considers active ingredients of plant protection products, but is also considered relevant to other chemicals. It is recommended that toxicity tests be conducted only up to the saturation limit, dispersants avoided, and solvents used only if necessary to support handling and speed of dissolution. Analytical measurements of exposure concentrations should reflect what organisms are exposed to. If acute toxicity testing at the saturation limit yields no adverse effects, further testing should not normally be required; the toxicity value of the endpoints should be considered as the saturation limit and adverse classification should not be required. Chronic testing, if required, should then be conducted at the practical saturation limit as this is the most realistic worst-case exposure scenario. If no adverse effects occur, the risk should be acceptable because higher aqueous exposure cannot occur. This could be substantiated by testing additional species. Assessment factors on no observed effect concentration (NOEC) values at the saturation limit require careful consideration in the risk assessment to avoid unnecessarily low regulatory acceptable concentrations.

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Section: Hematopoietic Stem Cellsmentioning

confidence: 99%

Aquatic toxicity tests with substances that are poorly soluble in water and consequences for environmental risk assessment

Weyman¹,

Rufli

Weltje³

et al. 2012

Environmental Toxicology and Chemistry

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“…MNCs were washed twice with PBS and resuspended in 600 μl PBS buffer containing 2 mM EDTA and 0.5% BSA. CD34 + cells were isolated using magnetically conjugated mouse anti-human CD34 beads (Miltenyi Biotec, Bergisch Gladbach, Germany) followed by separation on MS columns (Miltenyi Biotec) according to the manufacturer's instructions [28]. The purity of the CD34 + cells was 98.39% as determined by fluorescence-activated cell sorting (FACS).…”

Section: Isolation Of Human Ucb Cd34 + Cellsmentioning

confidence: 99%

Expression, Purification, and Characterization of a Novel Soluble Form of Human Delta-like-1

Zhao

Guo

et al. 2009

Appl Biochem Biotechnol

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The notch signaling pathway plays an important role in inhibiting cell differentiation and enhancing the repopulation capability of hematopoietic stem/progenitor cells. In this study, we developed rhDSL, a novel soluble form of Notch ligand Delta-like-1, which contains the DSL domain and the N-terminal sequence of the ligand, and investigated its function in ex vivo expansion of human umbilical cord blood (UCB)-primitive hematopoietic cells. The coding sequence for rhDSL was cloned into a pQE30 vector, and the recombinant rhDSL, fused with a 6x His tag, was expressed in Escherichia coli as inclusion bodies after isopropyl beta-D-thiogalactoside induction. After renaturing by dilutions, the protein was purified through anion exchange followed by affinity chromatography. The purity of rhDSL protein was more than 99% with very low endotoxin. In combination with human c-kit ligand, the effect of rhDSL on ex vivo expansion of UCB CD34(+) cells was found to be optimal at 1.5 microg/ml of rhDSL. The rhDSL protein might therefore be a potential supplement for the expansion of UCB-primitive hematopoietic cells.

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“…The network input and output data were obtained from literature 1,2,[4][5][6][7][8][9][10][11][12][14][15][16][17][18][19][20][21][22][23][24][25]27,[29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][46][47][48][49] for evaluation and prediction. Altogether 341 data were collected and summarized in Table 1.…”

Section: Network Input and Outputmentioning

confidence: 99%

Neural Network Analysis of Ex-vivo Expansion of Hematopoietic Stem Cells

Fan

Liu

et al. 2007

Ann Biomed Eng

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The shortage of hematopoietic stem cells (HSCs) greatly limits their widespread clinical applications. Few studies however, investigated the relationship between the cellular expansion and the influencing factors although wide variety results of the ex-vivo expansion of HSCs existed in literature. Here, a back-propagation (BP) neural network model was employed to evaluate the ex-vivo expansions of nuclear cells (NCs), CD34(+) cells, and colony-forming units (CFU-Cs), where the output was the cellular expansion folds and the inputs include inoculated density, cytokines, resources, serum, stroma, culture time, and bioreactor types. Around 124, 86, and 90 samples were used to train the neural network for the expansion evaluations of NCs, CD34(+ )cells, and CFU-Cs, respectively, while 17, 14, and 10 samples were applied to predict respectively. The results show that for the training of network, the interval accuracy of the expansion folds for the different cells is 85.5, 86.1, and 86.7%, respectively, while the truth-value accuracy is still up to 59.7, 50.0, and 62.2%, respectively within a relative error (RE) of +/-20%. For the prediction of network, the interval accuracy can be up to 82.4, 71.4, and 70%, respectively, while the truth-value accuracy is only 29.4, 14.3, and 50.0%, respectively (RE = +/-20%). Moreover, six verification experiments were carried out based on our interval predicted values and the results proved that the five group predicted conditions lead to the correct expansion of the HSCs with the accuracy more than 80%. Considering the complexity of HSC expansion and complicated wide range of the experimental data, such relatively high interval accuracy for training and prediction as well as verification are satisfied. Therefore this nonlinear modeling makes it possible to describe quantitatively the effects of the culture conditions on the HSC expansion and to predict the optimal culture conditions for higher ex-vivo expansion of HSCs.

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Ex vivo expansion of umbilical cord blood CD34⁺ cells in a closed system: a multicentric study

Cited by 11 publications

References 28 publications

Aquatic toxicity tests with substances that are poorly soluble in water and consequences for environmental risk assessment

Aquatic toxicity tests with substances that are poorly soluble in water and consequences for environmental risk assessment

Expression, Purification, and Characterization of a Novel Soluble Form of Human Delta-like-1

Neural Network Analysis of Ex-vivo Expansion of Hematopoietic Stem Cells

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Ex vivo expansion of umbilical cord blood CD34+ cells in a closed system: a multicentric study

Cited by 11 publications

References 28 publications

Aquatic toxicity tests with substances that are poorly soluble in water and consequences for environmental risk assessment

Aquatic toxicity tests with substances that are poorly soluble in water and consequences for environmental risk assessment

Expression, Purification, and Characterization of a Novel Soluble Form of Human Delta-like-1

Neural Network Analysis of Ex-vivo Expansion of Hematopoietic Stem Cells

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Product

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Ex vivo expansion of umbilical cord blood CD34⁺ cells in a closed system: a multicentric study