<i>Fasciola gigantica</i> cathepsin L proteinase‐based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis

Ježek, Jan; Ridi, Rashika El; Salah, Mohamed; Wagih, Amal; Aziz, Haidy W.; Tallima, Hatem; Shafie, Mohamed H. El; Khalek, Tarek Abdel; Ammou, Faten F. Abo; Strongylis, Constantinos; Moussis, Vassilios; Tsikaris, Vassilios

doi:10.1002/bip.20788

Cited by 18 publications

(7 citation statements)

References 30 publications

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“…[75] Subsequently, the synthetic polypeptides that represented the full-length mature protein sTS14 were assessed for serologic potential using ELISA for the diagnosis of NCC. [76] Since then, pure epitopes and molecules are being increasingly used as diagnostic antigens in a large number of parasitic infections including malaria,[77] Chagas’ disease,[78] leishmaniasis,[79] schistosomiasis,[80] cystic echinococcosis,[81] fascioliasis,[82] and cysticercosis. [83]…”

Section: Antigens Of the Cysticercimentioning

confidence: 99%

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Parija

Gireesh

2011

Trop Parasitol

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Neurocysticercosis (NCC) is difficult to diagnose clinically because of its varied clinical presentation. However, an accurate diagnosis is possible only after suspicion on epidemiological grounds, proper interpretation of the clinical data, analysis of the findings on imaging studies, and specific immunological tests on the serum and cerebrospinal fluid (CSF). The diagnosis of NCC by any single parameter thus continues to remain difficult. In the past, detection of NCC was based on autopsy studies and histological confirmation. In recent times, the advent of imaging methods such as computed tomography and magnetic resonance imaging have provided excellent non-invasive tools for easy detection of NCC. Nevertheless, an imaging technique of the brain, although useful, is not considered as a gold standard for the diagnosis of NCC. Serological tests are being increasingly used in adjunct with imaging techniques, to aid the diagnosis of NCC. Immunodiagnostic techniques include detection methods for specific antibodies and for circulating parasite antigens in the serum and CSF. Currently, many of the immunodiagnostic tests, including the enzyme-linked immunosorbent assay and enzyme immunotransfer blot, use purified native antigens for the immunodiagnosis of NCC. Nevertheless, the main problem with the use of native cysticercal antigens is that the native proteins often show cross reactions with sera from humans infected with other parasites. The preparation of native antigens also demand a constant supply of parasitic material from the intermediate host pig. In order to overcome the problems in using native antigens, the recombinant antigens or synthetic peptides, which can be produced under stable conditions, are being evaluated for the serodiagnosis of NCC.

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Section: Antigens Of the Cysticercimentioning

confidence: 99%

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Parija

Gireesh

2011

Trop Parasitol

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“…Despite such a large number of potential synthetic peptides having been identified, none are currently being marketed for human use [16] and few studies reported to date have used synthetic peptides as anti-helminth vaccines, including Echinococcus granulosus [17], Trichinella spiralis [18], Brugia malayi [19], Taenia solium [20], Schistosoma mansoni [21] and F. gigantica [22]. Regarding F. hepatica , synthetic peptides have been used in diagnosing human infections.…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Studies for Assessing the Immune Response and Protection-Inducing Ability Conferred by Fasciola hepatica-Derived Synthetic Peptides Containing B- and T-Cell Epitopes

et al. 2014

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Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains have appeared and developing an effective vaccine against this disease has increasingly become a priority. Several bioinformatics tools were here used for predicting B- and T-cell epitopes according to the available data for Fasciola hepatica protein amino acid sequences. BALB/c mice were immunised with the synthetic peptides by using the ADAD vaccination system and several immune response parameters were measured (antibody titres, cytokine levels, T-cell populations) to evaluate their ability to elicit an immune response. Based on the immunogenicity results so obtained, seven peptides were selected to assess their protection-inducing ability against experimental infection with F. hepatica metacercariae. Twenty-four B- or T-epitope-containing peptides were predicted and chemically synthesised. Immunisation of mice with peptides so-called B1, B2, B5, B6, T14, T15 and T16 induced high levels of total IgG, IgG1 and IgG2a (p<0.05) and a mixed Th1/Th2/Th17/Treg immune response, according to IFN-γ, IL-4, IL-17 and IL-10 levels, accompanied by increased CD62L+ T-cell populations. A high level of protection was obtained in mice vaccinated with peptides B2, B5, B6 and T15 formulated in the ADAD vaccination system with the AA0029 immunomodulator. The bioinformatics approach used in the present study led to the identification of seven peptides as vaccine candidates against the infection caused by Fasciola hepatica (a liver-fluke trematode). However, vaccine efficacy must be evaluated in other host species, including those having veterinary importance.

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“…Quite a number of relatively cheap antibody detection indirect enzyme-linked immunosorbent assays (ELISA) with high sensitivity and specificity have been developed. Most of these techniques are based on excretory-secretory products and cathepsin L proteases [70,73].…”

Section: Diagnosis Of Fascioliasismentioning

confidence: 99%

Fascioliasis: A Foodborne Disease of Veterinary and Zoonotic Importance

Atalabi¹,

Lawal²

2022

Rural Health

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Fascioliasis is a food-borne neglected disease caused by digenetic trematodes in the genus Fasciola. There is a significant increase in the global prevalence of human fascioliasis with a strong correlation with a high infection rate among ruminant definitive hosts. Fasciola is a liver fluke with complex life cycle. Fascioliasis is endemic in every continent of the world with the exception of Antarctica. Discharge of the metabolites of liver flukes into the circulatory system of hosts has pathological consequences. Fascioliasis has been diagnosed by parasitological, immunological, and molecular means, and it is being reliably treated chemotherapeutically. The emerging drug-resistant strains of liver flukes have led to the need for vaccine development. Most vaccine candidates were first isolated as native proteins from adult worms. Several of the early antigens, including cathepsin L proteases, Glutathione S-transferase (GST), and fatty acid binding protein (FABP), significantly reduced worm burden, egg output, and liver pathology in cattle and sheep. Climate change, emerging drug resistance, and the development of new parasite strains through hybridization are the current challenges that could potentially alter the epidemiology of fascioliasis soon. Therefore, researchers need to produce promising vaccines that offer maximum protection to farm animals and humans.

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Fasciola gigantica cathepsin L proteinase‐based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis

Abstract: ABSTRACT:Sheep fasciolosis is a devastating burden for the livestock industry. We herein report on immunodiagnosis of

Cited by 18 publications

References 30 publications

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

In Vitro and In Vivo Studies for Assessing the Immune Response and Protection-Inducing Ability Conferred by Fasciola hepatica-Derived Synthetic Peptides Containing B- and T-Cell Epitopes

Fascioliasis: A Foodborne Disease of Veterinary and Zoonotic Importance

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