Fistulinella ruschii, sp. nov., and a new record ofFistulinella campinaranaevar.scrobiculatafor the Atlantic Forest, Brazil

Abstract

Fistulinella is a small genus of boletoid fungi in the subfamily Austroboletoideae in the order Boletales. In this paper, F. ruschii from the Atlantic Forest is proposed as new to science and F. campinaranae var. scrobiculata, known from the Brazilian Amazon forest, is recorded for the first time in the Atlantic Forest. Macro- and microscopic descriptions, molecular data (nuc rDNA ITS1-5.8S-ITS2 and nuc 28S rDNA), photographs of the basidiomata, and scanning electron microscopy images of basidiospores are provided for both species. Based on sampling of six taxa, New World Fistulinella is found to be a strongly supported monophyletic group, but the genus at large is nonmonophyletic.

Outstanding Pinkish Brown-Spored Neotropical Boletes:Austroboletus subflavidusandFistulinella gloeocarpa(Boletaceae, Boletales) from the Dominican Republic

“…Sequences of Austroboletus betulae [3,86] were not included in the analyses because the species was recently inferred to belong in Aureoboletus Pouzar within the subfamily Xerocomoideae [5]. Bothia and Solioccasus sequences were used as outgroup according to Wu et al [7,8] and Magnago et al [42]. Phylogenetic trees were constructed with Bayesian inference (BI) and Maximum likelihood (ML) criteria.…”

“…Gomez & Singer and possibly with the sequestrate genus Carolinigaster M.E. Smith & S. Cruz [5,7,8,31,[37][38][39][40][41][42][43][44][45][46][47]. The former three genera, along with Austroboletus, have been accommodated in the subfamily Austroboletoideae G. Wu & Zhu L. Yang, as they cluster in an well-delimited grouping with respect to other lineages in the Boletaceae [7].…”

“…The former three genera, along with Austroboletus, have been accommodated in the subfamily Austroboletoideae G. Wu & Zhu L. Yang, as they cluster in an well-delimited grouping with respect to other lineages in the Boletaceae [7]. Despite the increasing number of morphologically and molecularly-based novel species assigned to Austroboletus in the last few years from Amazon Colombia [31], India [48,49] and Australia [26], this genus has been shown to represent a polyphyletic unit [5,7,31,37,42]. Moreover, the polyphyly of Austroboletus has been further highlighted by the recent separation of the genus Ionosporus O. Khmelnitsky, based on the Malaysian species Boletus longipes Massee [41].…”

“…On the other hand, preliminary molecular analyses suggested this genus to be polyphyletic [31,37] and accordingly an inclusive revision complemented by further sampling from different geographic regions aiming at a better understanding of its generic boundaries would be urgently needed, especially in relation to morphologically very close smooth-spored genera such as Mucilopilus and Ixechinus R. Heim ex R. Heim. Moreover Vasco-Palacios et al [31], and Magnago et al [42] have stressed that American species belonging in Fistulinella cluster in a statistically strongly supported separate clade with respect to those described from Australia and New Zealand, but it is not until molecular analyses are carried out on the generic type, the African taxon F. staudtii, that a taxonomic and geographic delimitation of Fistulinella s. str. lineage will be definitely clarified.…”

Longistriata flava (Boletaceae, Basidiomycota) – a new monotypic sequestrate genus and species from Brazilian Atlantic Forest

“…In Brazil, there are numerous surveys that have documented epigeous Boletales in exotic plantations and native forests (Rick 1961;Guzmán 1970;Putzke 1994;Watling and de Meijer 1997;Baseia and Milanez 2000;Giachini et al 2000;Baseia and Milanez 2002;Sobestiansky 2005;de Meijer 2006;Gurgel et al 2008;Cortez et al 2011;Magnago and Neves 2014;Magnago et al 2017aMagnago et al , 2017bMagnago et al 2018Magnago et al , 2019. However, information related to sequestrate hypogeous fungi is scanty (Sulzbacher et al 2016a).…”