<i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Consists of a Small Number of Divergent and Globally Distributed Clonal Lineages

Koenig, R.; Ploetz, R. C.; Kistler, H. Corby

doi:10.1094/phyto.1997.87.9.915

Cited by 144 publications

(131 citation statements)

References 25 publications

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“…The results of our study are similar in lines with those obtained by Kistler (1997) and Gherbawy (1999) who suggested that certain F. oxysporium isolates within a formae speciales may be genetically less similar to each other than those isolated as nonpathogenic strains or those isolated as a group in the other formae speciales (Vilgalys and Cubeta, 1994;Banniza et al, 1996;Kistler, 1997;Koening et al, 1997;O'Donnell et al, 1998;Neeraja et al, 2002 ;Runhua et al, 2002).…”

Section: C2-2supporting

confidence: 91%

Genetic Variability in the Isolates of Rhizoctonia solani Kühn, the Causal Agent of Sheath Blight of Rice by Using RAPD Markers

Nagaraju¹,

Naik²

2017

Int.J.Curr.Microbiol.App.Sci

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Section: C2-2supporting

confidence: 91%

Genetic Variability in the Isolates of Rhizoctonia solani Kühn, the Causal Agent of Sheath Blight of Rice by Using RAPD Markers

Nagaraju¹,

Naik²

2017

Int.J.Curr.Microbiol.App.Sci

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“…This clonal lineage consisted of only seven highly similar AFLP genotypes, and all of its members shared identical or nearly identical EF-1␣ and IGS rDNA sequences and possessed only MAT1-1 idiomorphs, indicating that they were of clonal origin. To date, molecular epidemiological studies that have identified fungal pathogens with a highly clonal population structure are restricted to a relatively small number of clinically (8,9), zoologically (17), and agriculturally (4, 5, 11) important species, including members of the FOC (13,29). However, most clinically important fungi investigated to date isolates indicated by shading.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Human Pathogenic Members of the Fusarium oxysporum Complex Inferred from Multilocus DNA Sequence Data and Amplified Fragment Length Polymorphism Analyses: Evidence for the Recent Dispersion of a Geographically Widespread Clonal Lineage and Nosocomial Origin

O’Donnell

Sutton²,

Rinaldi³

et al. 2004

J Clin Microbiol

213

171

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Fusarium oxysporum is a phylogenetically diverse monophyletic complex of filamentous ascomycetous fungi that are responsible for localized and disseminated life-threatening opportunistic infections in immunocompetent and severely neutropenic patients, respectively. Although members of this complex were isolated from patients during a pseudoepidemic in San Antonio, Tex., and from patients and the water system in a Houston, Tex., hospital during the 1990s, little is known about their genetic relatedness and population structure. This study was conducted to investigate the global genetic diversity and population biology of a comprehensive set of clinically important members of the F. oxysporum complex, focusing on the 33 isolates from patients at the San Antonio hospital and on strains isolated in the United States from the water systems of geographically distant hospitals in Texas, Maryland, and Washington, which were suspected as reservoirs of nosocomial fusariosis. In all, 18 environmental isolates and 88 isolates from patients spanning four continents were genotyped. The major finding of this study, based on concordant results from phylogenetic analyses of multilocus DNA sequence data and amplified fragment length polymorphisms, is that a recently dispersed, geographically widespread clonal lineage is responsible for over 70% of all clinical isolates investigated, including all of those associated with the pseudoepidemic in San Antonio. Moreover, strains of the clonal lineage recovered from patients were conclusively shown to genetically match those isolated from the hospital water systems of three U.S. hospitals, providing support for the hypothesis that hospitals may serve as a reservoir for nosocomial fusarial infections. Members of the phylogenetically diverse monophyleticFusarium oxysporum complex (FOC) are best known as cosmopolitan soilborne plant pathogens that are responsible for economically devastating vascular wilts of an enormous range of agronomically important plant hosts (6). Members of the FOC are also frequently isolated from nonplant sources, particularly from the soil but also from air and animals. Over the past 2 decades, however, fusaria have emerged as opportunistic pathogens causing life-threatening disseminated infections in immunocompromised patients (3). In patients who are persistently neutropenic, deeply invasive fusarial infections cause 100% mortality (18). Most localized and disseminated cases of fusariosis are caused by members of the Fusarium solani species complex, followed by members of the FOC (1). Fortunately, the recent development of one strain of F. oxysporum as a model system will greatly facilitate the molecular genetic dissection of fungal virulence determinants during plant and animal pathogenesis (24).Although molecular epidemiological studies have been completed for nosocomial fusariosis (1, 25), most of the analyses were conducted on members of the F. solani species complex.

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“…Finally, various DNA-based tools have been used to separate F. oxysporum f. sp. cubense into a number of clonal lineages that more or less correspond to their grouping based on VCGs (6,22,38,59).…”

mentioning

confidence: 99%

Evolutionary Relationships among theFusarium oxysporumf. sp.cubenseVegetative Compatibility Groups

Fourie

Steenkamp

Gordon

et al. 2009

Appl Environ Microbiol

145

204

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Fusarium oxysporum f. sp. cubense, the causal agent of fusarium wilt of banana (Musa spp.), is one of the most destructive strains of the vascular wilt fungus F. oxysporum. Genetic relatedness among and within vegetative compatibility groups (VCGs) of F. oxysporum f. sp. cubense was studied by sequencing two nuclear and two mitochondrial DNA regions in a collection of 70 F. oxysporum isolates that include representatives of 20 VCGs of F. oxysporum f. sp. cubense, other formae speciales, and nonpathogens. To determine the ability of F. oxysporum f. sp. cubense to sexually recombine, crosses were made between isolates of opposite mating types. Phylogenetic analysis separated the F. oxysporum isolates into two clades and eight lineages. Phylogenetic relationships between F. oxysporum f. sp. cubense and other formae speciales of F. oxysporum and the relationships among VCGs and races of F. oxysporum f. sp. cubense clearly showed that F. oxysporum f. sp. cubense's ability to cause disease on banana has emerged multiple times, independently, and that the ability to cause disease to a specific banana cultivar is also a polyphyletic trait. These analyses further suggest that both coevolution with the host and horizontal gene transfer may have played important roles in the evolutionary history of the pathogen. All examined isolates harbored one of the two mating-type idiomorphs, but never both, which suggests a heterothallic mating system should sexual reproduction occur. Although, no sexual structures were observed, some lineages of F. oxysporum f. sp. cubense harbored MAT-1 and MAT-2 isolates, suggesting a potential that these lineages have a sexual origin that might be more recent than initially anticipated.

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Fusarium oxysporum f. sp. cubense Consists of a Small Number of Divergent and Globally Distributed Clonal Lineages

Cited by 144 publications

References 25 publications

Genetic Variability in the Isolates of Rhizoctonia solani Kühn, the Causal Agent of Sheath Blight of Rice by Using RAPD Markers

Genetic Variability in the Isolates of Rhizoctonia solani Kühn, the Causal Agent of Sheath Blight of Rice by Using RAPD Markers

Genetic Diversity of Human Pathogenic Members of the Fusarium oxysporum Complex Inferred from Multilocus DNA Sequence Data and Amplified Fragment Length Polymorphism Analyses: Evidence for the Recent Dispersion of a Geographically Widespread Clonal Lineage and Nosocomial Origin

Evolutionary Relationships among theFusarium oxysporumf. sp.cubenseVegetative Compatibility Groups

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