<i>Getting It Wrong: The Medieval Epistemology of Error</i>. G. R. Evans

Levy, Ian Christopher

doi:10.1017/s0038713400168915

Cited by 1 publication

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“…This preference was not evident with corn oil, because in this case the particle lipids are poor in long-chain n-3 PUFA, and because the triacylglycerols derived from corn oil are more homogeneous than those derived from fish oil with respect to molecular species composition. This is in agreement with previous reports showing that the in vitro activity of LPL from cardiac (21) and other tissues (24) is highest on chylomicron triacylglycerols containing 18:0, 18:2n-6 or 18:3n-3 and lowest when this lipid class contains 20:4n-6, 20:5n-3 or 22:6n-3.…”

Section: Discussionsupporting

confidence: 93%

Influencia de la composición en ácidos grasos de los remanentes de quilomicrones derivados de aceite de pescado o de maíz en el perfil lipídico de los hepatocitos de rata en cultivo

Maldonado

Chico

Botham

et al. 2003

J. Physiol. Biochem.

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The aim of this work was to characterise the lipid and fatty acid composition of chylomicron remnants enriched in n-3 or n-6 polyunsaturated fatty acids (PUFA) and to investigate their influence on the fatty acid profiles of the lipids of rat hepatocytes cultured in monolayers. Chylomicrons were prepared from the lymph collected from the thoracic duct of rats given an oral dose of fish or corn oil (high in n-3 and n-6 PUFA, respectively), and remnants were prepared in vitro from such chylomicrons using rat plasma containing lipoprotein lipase. The fatty acids predominating in the oils abounded also in their respective chylomicrons and remnants, especially in triacylglycerols. Chylomicrons as well as remnants contained small amounts of phospholipids and long-chain PUFA that were minor in, or absent from, the dietary oils, evidently provided by the intestinal epithelium. The incubation of hepatocytes for 6 h, with either n-3 or n-6 PUFA-rich remnants (0.25-0.75 mM triacylglycerol) resulted in a dose-dependent increase in the amount of triacylglycerols and phospholipids in the cells, which was not affected further by increasing the incubation time to 19 h. Whereas hepatocyte triacylglycerols mostly incorporated the PUFA predominating in each remnant type, the fatty acid profile of cell phospholipids was virtually unchanged. In addition, irrespective of whether they were enriched in n-3 or n-6 PUFA, remnants promoted a relative decrease in the amount of cholesteryl esters, a minor hepatocyte lipid class poor in PUFA. The results demonstrate that the hepatocyte fatty acid profile is modulated in a lipid-class specific way by the amount and type of dietary PUFA delivered to cells in chylomicron remnants.

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Section: Discussionsupporting

confidence: 93%