Globisporangium coniferarum sp. nov ., associated with conifers and Quercus spp.

Abstract

During a survey of gardens in Shiraz County, Iran, aimed at identifying oomycetes associated with roots of ornamental trees, a species of Globisporangium with distinctive morphological characters separating it from other known species in this genus was recovered from conifers and occasionally from a Quercus sp. Five isolates of this species were characterised. Phylogenetic analyses of nuclear (ITS and βtub) and mitochondrial (cox1 and cox2) loci using Bayesian inference and maximum likelihood analyses as well as their distinct morphological and cultural characteristics (e.g., abundant production of chlamydospores; globose, ellipsoid to ovoid sporangia; amorphous oogonia with a smooth wall; paragynous to rarely hypogynous antheridia and 1–5 antheridia per oogonium; mostly plerotic oospores) revealed that these isolates belong to a new Globisporangium species grouping in the phylogenetic clade G of Pythium sensu lato. This paper formally describes Globisporangium coniferarum sp. nov. as a new species and compares it with other phylogenetically related and already known Globisporangium species, including G. nagaii, G. violae, G. paddicum, G. okanoganense, G. iwayamae and G. canariense.

<em>Pythium banihashemianum </em>sp. nov. and <em>Globisporangium izadpanahii </em>sp. nov. Two New Oomycete Species from Rice Paddies in Iran

“…The presence of multiple divergent copies of the ITS region is a well-known phenomenon among Pythium s.l. species and was reported previously for G. coniferarum [55]. Analyzing Globisporangium izadpanahii sp.…”

“…nov., G. coniferarum produces ovoid to ellipsoid sporangia with vesicles and zoospores [55]. Furthermore, one of the key characteristics of G. coniferarum is the production of abundant chlamydospores and different shapes of oogonia (from globose to ovoid and ellipsoid) [55], while in G. izadpanahii sp. nov. isolates chlamydospores and oogonia were exclusively globose.…”

“…Sequences were deposited into GenBank. For lowquality ITS sequences, cloning was performed using Strata Cloning Kit (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer's instruction [55].…”

Pythium banihashemianum sp. nov. and Globisporangium izadpanahii sp. nov.: Two New Oomycete Species from Rice Paddies in Iran

“…nov. , G. coniferarum produces ovoid to ellipsoid sporangia with vesicles and zoospores [ 54 ]. Furthermore, one of the key characteristics of G. coniferarum is the production of abundant chlamydospores and different shapes of oogonia (from globose to ovoid and ellipsoid) [ 52 ], while in G. izadpanahii sp. nov. chlamydospores and oogonia were exclusively globose.…”

“…Sequences were deposited into GenBank. For low-quality ITS sequences, cloning was performed using the Strata Cloning Kit (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instruction [ 52 ].…”