Glycosylphosphatidyl inositol‐anchored protein (GPI‐80) gene expression is correlated with human thymoma stage

Abstract

Thymoma is one of the most common solid tumors in the mediastinum. Because there is no typical cell line for human thymoma, the development and use of molecular-based therapy for thymoma will require detailed molecular-genetic analysis of patients' tissues. Recent reports showed that genetic aberrations in thymoma were most frequently seen in chromosome 6q regions. We investigated the use of oligonucleotide arrays to monitor in vivo expression levels of genes in chromosome 6 regions in early- hymoma is a neoplasm of thymic epithelial cells mixed with lymphocytes. Although thymomas are usually encapsulated, some are characterized by locally invasive growth, pleural dissemination, or extrathoracic metastasis. However, there seems to be no cytological difference between noninvasive thymoma and invasive/metastatic thymoma.1-3) Several approaches have been made to differentiate non-invasive thymoma from invasive/metastatic thymoma, but the issue still remains controversial. Malignancy is generally evaluated in terms of the tumor's macroscopic invasiveness seen at the time of surgery.The elucidation of the genetic events underlying the initiation and progression of human thymoma has been hampered by limitations inherent in both in vitro and in vivo methods of study. The most significant limitation of in vitro-based systems is that there is no typical cell line for human thymoma. Also, genetic information derived from cell lines may not accurately reflect the molecular events taking place in the tissues from which they were derived. Examining tumors for alterations in gene expression is a potentially useful approach to identifying molecular differences between early-and late-stage thymomas. The advent of high-density oligonucleotide microarray technology, 4) with its capacity for simultaneous monitoring of thousands of genes, provides a unique opportunity for high-throughput genetic analysis of tumors. [5][6][7][8][9][10][11][12]

Glycoproteomic analysis of WGA‐bound glycoprotein biomarkers in sera from patients with lung adenocarcinoma

“…It has also been determined to be secreted outside of cells or tissues. GPI-80 was found to be up-regulated in thymic carcinoma (stage IV) and leukemia and has a role in the regulation of cell adhesion and migration [53][54][55]. Likewise, our results showed that GPI-80 expression was increased in lung cancer serum samples.…”

Comprehensive Genomic Analysis Reveals Clinically Relevant Molecular Distinctions between Thymic Carcinomas and Thymomas

“…The lack of findings could be due to the low number of samples analyzed. We also were not able to compare expression data from tumors with that from matched normal thymus samples, which was unavailable, as in other studies [29, 47]. Finally, especially for type B2 thymomas, results may have been confounded by the presence of lymphocytic infiltrates, although we did not identify any differences in the profiles depending on the epithelial tumor cell content.…”

Arg and DAP3 expression was correlated with human thymoma stage

“…Examining tumors for alternations in gene expression is a potentially useful approach to identify the molecular differences between early-and late-stage thymomas. The advent of high-density oligonucleotide microarray technology [4], with its capacity for simultaneous monitoring of thousands of genes, provides a unique opportunity for high-throughput genetic analysis of tumor [5][6][7][8][9][10], including for thymomas [8][9][10]. Recent report showed that the gain of chromosome 1q was frequent in thymoma [11], however, responsible gene has not yet been reported.…”