GST (GSTM1, GSTT1, and GSTP1) polymorphisms in the genetic susceptibility of Turkish patients to cervical cancer

Abstract

Objective: This work investigates the role of glutathione S-transferase M1 (GSTM1), glutathione S-transferase T1 (GSTT1), and glutathione S-transferase P1 (GSTP1) enzymes and polymorphisms, which are found in phase II detoxification reactions in the development of cervical cancer. Methods: This study was conducted with 46 patients diagnosed with cervical cancer and 52 people with no cancer history. Multiplex PCR methods were used to evaluate the GSTM1 and GSTT1 gene polymorphism. However, the GSTP1 (Ile105Val) gene polymorphism was studied using a PCR-RFLP method. The patient and control groups were compared using a chi-square test with p<0.05. Results: In the patient group, statistical significance was determined for gravidity (p=0.03), parity (p=0.01), and the number of living children (p=0.01) compared to the control group. The gene frequency of GSTM1, GSTT1, and GSTP1 polymorphisms was evaluated. We observed that GSTM1 and GSTT1 null genotype frequencies were 54.3% and 32.6% respectively, while GSTP1 (Ile/Val), (Ile/Ile), (Val/Val) genotype frequencies were 52%, 44%, and 4%, respectively, in the cervical cancer patients. No statistical variation was determined between the control and patient groups in terms of GSTM1, GSTT1, and GSTP1 polymorphisms (p>0.05). Conclusion:Our results demonstrate that GSTT1, GSTM1, and GSTP1 polymorphisms are not associated with cervical cancer in Turkish patients.

Genetic polymorphisms of glutathione S transferase and cervical intraepithelial neoplasia

“…A study taking into account 150 smoking women diagnosed with cervical neoplasia showed significant association with an increased risk of 4.19-folds of developing cervical cancer in association of GSTM1 null genotypes (40). Other studies confirm that there is a positive association between active or passive smoking, GST null genotypes and the risk of developing cervical cancer (27,28).…”

“…In the same meta-analysis, smoking is linked between GSTM1 null genotype and cervical cancer (27). In contrast, other studies show that polymorphisms in GSTM1 and GSTT1 genes are not associated with a higher risk for cervical cancer in HPV infected women (16,28). In our study, the GST M1 null genotype was a significant protecting factor for CIS (OR=0.1220, χ 2 =5.25, p=0.0219), while GST T-/M+ genotype was associated with a significantly increased risk for developing LSIL and CIS (OR=7.2592, p=0.0203, and OR=4.6666, p=0.0314, respectively).…”

“…Among these, genetic polymorphisms of GST genes were intensely studied in relationship to chronic hrHPV infections and cervical cancer, but results are still inconclusive even if large populations with different ethnicities were included (27,28). GST polymorphisms have been previously analyzed in study lots from Transylvania in relationship with other pathologies such as glaucoma and lung cancer (29,30).…”

Glutathione S-transferase M1 and T1 polymorphisms and cervical cancer risk: A meta-analysis

“…According to the exclusion criteria, 15 articles were excluded including 5 articles containing overlapping population [7][8][9][10][11], 8 precancerous lesions included in the cases [12][13][14][15][16][17][18][19], 2 without sufficient data [20][21]. At last, data were available from 16 individual case-control studies [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37], Table 1 presented characteristics of these 16 case-control studies (a total of 1,627 CC cases and 2,161 controls). 14 studies on GSTM1 polymorphism (a total of 1,514 CC cases and 1,907 controls), 12 studies on GSTT1 polymorphism (a total of 1,187 CC cases and 1,590 controls), and 6 studies on GSTM1-GSTT1 interaction analysis (a total of 791 CC cases and 767controls) were included in the meta-analysis.…”

“…Compared with Economopoulos et al 's meta-analysis, our meta-analysis had some differences. Firstly, our meta-analysis included three new eligible studies published in Chinese [22][23][24] and two new case-control studies [25][26] in English in 2010, and excluded four studies [13,16,18,19] that had precancerous lesions patients in cases. Secondly, Asians (including Chinese, Indians, Koreans, Japanese and Thais), Caucasians and Latinos were stratified by ethnicity in our meta-analysis, while Chinese (including Koreans, Japanese and Thais) and non-Chinese were stratified GSTM1 AND GSTT1 POLYMORPHISMS AND CERVICAL CANCER RISK Fig.…”