<i>hace1</i> Influences zebrafish cardiac development via ROS‐dependent mechanisms

Razaghi, Babak; Steele, Shelby L.; Prykhozhij, Sergey V.; Stoyek, Matthew R.; Hill, Jennifer G.; Cooper, Matthew; McDonald, Lindsay; Lin, William Haoyang; Daugaard, Mads; Crapoulet, Nicolas; Chacko, Simi; Lewis, Stephen M.; Scott, Ian C.; Sorensen, Poul H.; Berman, Jason N.

doi:10.1002/dvdy.24600

Cited by 23 publications

(18 citation statements)

References 70 publications

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“…ROS participate in the development of the nervous system, the differentiation and formation of the nerve, and the development of lung and vascular tissue [38]. Recently, an article reported that high levels of ROS lead to heart looping disorder during heart development [39,40]. There are also Fig.…”

Section: Discussionmentioning

confidence: 99%

Isoniazid causes heart looping disorder in zebrafish embryos by the induction of oxidative stress

Wang

Wei

et al. 2020

BMC Pharmacol Toxicol

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Background: The cardiotoxicity of isoniazid on zebrafish embryos and its underlying mechanism is unclear. Methods: Here, we exposed zebrafish embryos at 4 h post-fertilization to different levels of isoniazid and recorded the morphology and number of malformed and dead embryos under the microscope. Results: The high concentration of isoniazid group showed more malformed and dead embryos than the low concentration of isoniazid group and control group. The morphology of the heart and its alteration were visualized using transgenic zebrafish (cmlc2: GFP) and confirmed by in situ hybridization. The negative effects of isoniazid on the developing heart were characterized by lower heart rate and more heart looping disorders. Mechanistically, PCR showed decreased expression of heart-specific transcription factors when exposed to isoniazid. Oxidative stress was induced by isoniazid in cardiomyocytes, mediated by decreased activities of catalase and superoxide dismutase, which were rescued by scavengers of reactive oxygen species. Conclusion: In conclusion, this study demonstrated that isoniazid led to heart looping disturbance by the downregulation of cardiac-specific transcription factors and induction of cardiomyocyte apoptosis.

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Section: Discussionmentioning

confidence: 99%

Isoniazid causes heart looping disorder in zebrafish embryos by the induction of oxidative stress

Wang

Wei

et al. 2020

BMC Pharmacol Toxicol

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“…Thus they suggest that phenotypes in xenopus could be linked to the maternal pool of RNA. Recently, a study demonstrated that HACE1 is involved in the normal development and proper function of the heart in zebrafish [77]. However, authors used splice-site morpholinos suggesting that this cardiac phenotype could be due to later zygotic expression of HACE1.…”

Section: Discussionmentioning

confidence: 99%

Domestication may affect the maternal mRNA profile in unfertilized eggs, potentially impacting the embryonic development of Eurasian perch (Perca fluviatilis)

et al. 2019

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Domestication is an evolutionary process during which we expect populations to progressively adapt to an environment controlled by humans. It is accompanied by genetic and presumably epigenetic changes potentially leading to modifications in the transcriptomic profile in various tissues. Reproduction is a key function often affected by this process in numerous species, regardless of the mechanism. The maternal mRNA in fish eggs is crucial for the proper embryogenesis. Our working hypothesis is that modifications of maternal mRNAs may reflect potential genetic and/or epigenetic modifications occurring during domestication and could have consequences during embryogenesis. Consequently, we investigated the trancriptomic profile of unfertilized eggs from two populations of Eurasian perch. These two populations differed by their domestication histories (F1 vs. F7+–at least seven generations of reproduction in captivity) and were genetically differentiated (FST = 0.1055, p<0.05). A broad follow up of the oogenesis progression failed to show significant differences during oogenesis between populations. However, the F1 population spawned earlier with embryos presenting an overall higher survivorship than those from the F7+ population. The transcriptomic profile of unfertilized eggs showed 358 differentially expressed genes between populations. In conclusion, our data suggests that the domestication process may influence the regulation of the maternal transcripts in fish eggs, which could in turn explain differences of developmental success.

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“…It participated in the development of the nervous system, promoting the differentiation and the formation of the nerve, and the development of lung and vascular (32). Recently, an article reported that high levels of ROS lead to heart looping disorder during heart development (33,34). There were also many reports that environmental poisons and drugs such as doxorubicin (35) and chlorpyrifos(36) caused abnormal development of various organs by inducing ROS accumulation.…”

Section: Discussionmentioning

confidence: 99%

Isoniazid causes heart looping disorder of zebrafish embryo by inducing oxidative stress

Wang¹,

Liu²,

Wei³

et al. 2019

Preprint

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The cardiotoxicity of isoniazid on zebrafish embryos and its underlying mechanism remained unclear. Here, we exposed zebrafish embryos at 4 hours post fertilization to different levels of isoniazid and recorded the morphology and number of malformed and dead embryos under the microscope. The high concentration of isoniazid group showed more malformed and dead embryos compared with low dose of isoniazid group and control group. Besides, the morphology of heart and its alteration were visualized using the transgenic zebrafish (cmlc2: GFP) and confirmed by in situ hybridization. The negative effects of isoniazid on the developing heart were characterized by lower heart rate and more heart looping disorders. Mechanistically, PCR showed decreased expression of heart-specific transcription factors exposed to isoniazid. Oxidative stress was induced by Isoniazid in cardiomyocytes, mediated by decreased activity of CAT and SOD, which could be rescued by ROS scavenger. In conclusion, we demonstrated that isoniazid lead to heart looping disturbance by downregulating cardiac specific transcription factors and inducing cardiomyocytes apoptosis.

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hace1 Influences zebrafish cardiac development via ROS‐dependent mechanisms

Abstract: Our study demonstrates that HACE1 is critical in the normal development and proper function of the vertebrate heart via a ROS-dependent mechanism. Developmental Dynamics 247:289-303, 2018. © 2017 Wiley Periodicals, Inc.

Cited by 23 publications

References 70 publications

Isoniazid causes heart looping disorder in zebrafish embryos by the induction of oxidative stress

Isoniazid causes heart looping disorder in zebrafish embryos by the induction of oxidative stress

Domestication may affect the maternal mRNA profile in unfertilized eggs, potentially impacting the embryonic development of Eurasian perch (Perca fluviatilis)

Isoniazid causes heart looping disorder of zebrafish embryo by inducing oxidative stress

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