Haemonchus contortusexcretory and secretory proteins (HcESPs) suppress functions of goat PBMCsin vitro

Abstract

Excretory and secretory products (ESPs) of nematode contain various proteins which are capable of inducing the instigation or depression of the host immune response and are involved in the pathogenesis of the worms. In the present study, Haemonchus contortus excretory and secretory products (HcESPs) were collected from the adult worms. Binding of HcESPs to goat peripheral blood mononuclear cells (PBMCs) was confirmed by immune-fluorescence assay. Effects of the HcESPs on cytokine production, cell proliferation, cell migration and nitric oxide (NO) production of PBMCs were checked by co-incubation of HcESPs with goat PBMCs. The results indicated that the production of IL-4 and IFN-γ were significantly decreased by HcESPs in dose dependent manner. On the contrary, the production of IL-10 and IL-17 were increased. Cell migration was significantly enhanced by HcESPs, whereas, HcESPs treatment significantly suppressed the cell proliferation and NO production. These results indicated that the HcESPs played important suppressive regulatory roles on PBMCs and provided highlights to the understanding of the host-parasite interactions.

Recombinant Haemonchus contortus 24 kDa excretory/secretory protein (rHcES-24) modulate the immune functions of goat PBMCs in vitro

“…In our recent study we found that HcESPs decreased the IL-4 production in vitro [19] , Contrary to that, in the present study, we identified that the rHcES-24 could enhance the secretion of IL-4. It indicated that this protein might be initiated the type 2 immune response against the infection of H. contortus .…”

Y75B8A.8 (HC8) protein of Haemonchus contortus: A functional inhibitor of host IL‐2

“…It was reported that two C‐type lectin receptors, namely mannose receptor (MR) and Dectin‐1, of Fasciola hepatica could partially inhibit the immunomodulatory effects of FhESPs on macrophages by binding to FhESPs . Similar to this, in our previous studies, we found that excretory and secretory products of H contortus (HcESPs) containing various proteins could bind to goat peripheral blood mononuclear cells (PBMCs) in vivo at different developmental stages, and HcESPs could inhibit the functions of goat PBMCs in vitro overall . All of the above research suggested that HcESPs play a central role in parasite immune evasion or the regulation of host immune responses.…”

“…The cell proliferation assay of the PBMCs was tested by using a cell counting kit‐8 assay reagent (Beyotime Biotechnology, Haimen, Jiangsu, China) . PBMCs (1 × 10 6 cells/mL) were inoculated in 96‐well plates (100 μL/well) and treated with rIL‐2 (5 μg/mL) and rHCGB (10, 20 and 40 μg/mL) or rHC8 (10, 20 and 40 μg/mL), respectively.…”

Recombinant elongation factor 1 alpha of Haemonchus contortus affects the functions of goat PBMCs

“…The pro‐inflammatory cytokine IL‐17 was secreted by the Th17 cells and related to pathogenesis of numerous helminths parasite 52‐54 . A previous study demonstrated that HcESPs were capable to induce IL‐17 cytokine production in PBMCs 24 . In this research, the results demonstrated that rHcEF‐1α could significantly promote IL‐17 production.…”

“…Previously, in the proteomic analysis of ESPs from H contortus , EF‐1α protein was recognized as binding molecule to goat PBMCs at L4, L5 and early adult developmental stages of H contortus in vivo 23 . Meanwhile, the immuno‐suppressant role of HcESPs on goat PBMCs in vitro was also demonstrated 24 . In recent studies, various binding proteins of H contortus were evaluated for their immune‐modulatory properties on goat PBMCs in vitro 25‐29 .…”