<i>Haemophilus parasuis</i> α-2,3-sialyltransferase-mediated lipooligosaccharide sialylation contributes to bacterial pathogenicity

Wang, Huan; Liu, Lu; Cao, Qi; Mao, Weiting; Zhang, Yage; Qu, Xinyi; Cai, Xuwang; Lv, Yujin; Chen, Huanchun; Xu, Xiaojuan; Wang, Xiangru

doi:10.1080/21505594.2018.1502606

Cited by 15 publications

(19 citation statements)

References 54 publications

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“…However, the serum resistance of K. pneumoniae serotype K1 did not exhibit any clear difference when the crp gene was deleted (Ou et al, 2017). Several hypotheses concerning the mechanism of this serum resistance have been proposed, such as decreased binding of IgG or increased binding of host complement alternative pathway inhibitor, fH (Wang et al, 2018). Thus, we hypothesize that the difference may be caused by a variation in the ability of the crp gene to bind IgG or fH in different bacteria.…”

Section: Discussionmentioning

confidence: 79%

Effect of cAMP Receptor Protein Gene on Growth Characteristics and Stress Resistance of Haemophilus parasuis Serovar 5

Jiang

Cheng

Cao

et al. 2020

Front. Cell. Infect. Microbiol.

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Haemophilus parasuis (HPS), a member of the family Pasteurellaceae, is a common bacteria in the upper respiratory tract of pigs but under certain circumstances can cause serious systemic disease (Glasser's disease) characterized by severe infection of the upper respiratory tract, fibrinous polyserositis, polyarthritis, and meningitis. cAMP receptor protein (CRP) is among the most important global regulators, playing a vital role in adapting to environmental changes during the process of bacterial infection. In order to investigate the function of the crp gene in the growth characteristics of H. parasuis serovar 5 (HPS5) and its ability to overcome adverse environmental stresses, a crp mutant strain (crp) was constructed and verified. In this study, we found that the crp gene was involved in growth rate, biofilm formation, stress tolerance, serum resistance, and iron utilization. Compared with the wild type, both the growth rate of the crp mutant and its resistance to osmotic pressure decreased significantly. Similar phenomena were also found in biofilm formation and iron utilization. However, the resistance to heat shock and serum complement of the crp mutant were enhanced. This study aimed to reveal the function in growth characteristics and stress resistance of the crp gene in HPS5. Whether it relates to virulence requires additional in-depth research.

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Section: Discussionmentioning

confidence: 79%

Effect of cAMP Receptor Protein Gene on Growth Characteristics and Stress Resistance of Haemophilus parasuis Serovar 5

Jiang

Cheng

Cao

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Most mucins have a high sialic acid content. Interestingly, systemic isolates of H. parasuis present the sialyltransferase-encoding gene lsgB gene, which is involved in the terminal sialylation of the lipooligosacharide (LOS), with roles in host cell adhesion and serum resistance [30, 31]. LOS sialylation is a molecular mimicry mechanism extensively used by bacteria to evade the host immune system.…”

Section: Discussionmentioning

confidence: 99%

Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins

Costa-Hurtado

Garcia-Rodriguez

López-Serrano

et al. 2019

Vet Res

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Haemophilus parasuis is part of the microbiota of the upper respiratory tract in swine. However, virulent strains can cause a systemic disease known as Glässer’s disease. Several virulence factors have been described in H. parasuis including the virulence-associated trimeric autotransporters (VtaAs). VtaA2 is up-regulated during infection and is only found in virulent strains. In order to determine its biological function, the vtaA2 gene was cloned with its native promotor region in pACYC184, and the transformed Escherichia coli was used to perform functional in vitro assays. VtaA2 was found to have a role in attachment to plastic, mucin, BSA, fibronectin and collagen. As other VtaAs from H. parasuis, the passenger domain of VtaA2 contains collagen domains. In order to examine the contribution of the collagen repeats to VtaA2 function, a recombinant vtaA2 without the central collagen domains was obtained and named vtaA2OL. VtaA2OL showed similar capacity than VtaA2 to adhere to plastic, mucin, BSA, fibronectin and plasma but a reduced capacity to adhere to collagen, suggesting that the collagen domains of VtaA2 are involved in collagen attachment. No function in cell adhesion and invasion to epithelial alveolar cell line A549 or unspecific binding to primary alveolar macrophages was found. Likewise VtaA2 had no role in serum or phagocytosis resistance. We propose that VtaA2 mediates adherence to the host by binding to the mucin, found in the upper respiratory tract mucus, and to the extracellular matrix proteins, present in the connective tissue of systemic sites, such as the serosa.

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“…Sialic acid is degraded into N-acetylmannosamine (ManNAc) by the action of sialic acid aldolase (NanA); then converted into fructose 6-phosphate by the action of NanK, NanE, NagB, and NagA proteins; and finally participates in the glycolytic pathway [66,67]. In non-typeable H. influenzae, sialylation of lipooligosaccharide (LOS) can endow bacteria with the ability of adhesion, invasion, and immune escape and play an important role in the pathogenic process [68]. Moreover, non-typeable H. influenzae can ingest dietary sialic acid to modify LOS on its surface and protect it from the host's adaptive immune factors and innate immune factors [69].…”

Section: Effect Of Sialic Acid On Oral Microbesmentioning

confidence: 99%

“…Moreover, non-typeable H. influenzae can ingest dietary sialic acid to modify LOS on its surface and protect it from the host's adaptive immune factors and innate immune factors [69]. In H. parasuis, the α-2,3 sialyltransferase can sialylate the terminal galactose of LOS via CMP-NeuAc or other donors [68].…”

Section: Effect Of Sialic Acid On Oral Microbesmentioning

confidence: 99%

Metabolic fate of dietary sialic acid and its influence on gut and oral bacteria

Zhang

Qiang

et al. 2021

Syst Microbiol and Biomanuf

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In recent years, the biological functions of human milk oligosaccharides and the potential toxic effects of red meat on human health have attracted considerable attention. Sialic acid is an important carbohydrate in milk and red meat, corresponding to sialylated oligosaccharides and N-glycolylneuraminic acid (NeuGc, one type of sialic acid). Herein, we reviewed the metabolic fate of dietary sialic acid in the body and their effects on gut and oral microbes. In summary, dietary NeuAc monomer is directly excreted through urine after being assimilated through the intestines and is not utilized by the human body; in contrast, dietary NeuGc from red meat is easily utilized by the human body and can be incorporated into the brain and other organs. Sialoglycans can be partially utilized by the human body, but they do not affect the cognitive development and growth of children. Dietary sialic acid may mainly regulate the growth and metabolism of gastrointestinal microbiota and human health and development through the gut-brain axis.

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Haemophilus parasuis α-2,3-sialyltransferase-mediated lipooligosaccharide sialylation contributes to bacterial pathogenicity

Cited by 15 publications

References 54 publications

Effect of cAMP Receptor Protein Gene on Growth Characteristics and Stress Resistance of Haemophilus parasuis Serovar 5

Effect of cAMP Receptor Protein Gene on Growth Characteristics and Stress Resistance of Haemophilus parasuis Serovar 5

Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins

Metabolic fate of dietary sialic acid and its influence on gut and oral bacteria

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